K-FGLQR

SKU: 700007621

50 assays (manual) / 500 assays (auto-analyzer)

Content:	50 assays (manual) / 500 assays (auto-analyzer)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels

Analyte:	D-Fructose, D-Glucose
Assay Format:	Spectrophotometer, Auto-analyzer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	2.5 to 150 µg of D-glucose plus D-fructose per assay
Limit of Detection:	6 mg/L
Limit of Quantification:	16 mg/L
Reproducibility (%):	< 5%
Reaction Time (min):	~ 15 min for Total Sugars

The D-Fructose/D-Glucose (Liquid Ready™) assay kit is a method for the specific measurement and analysis of D-Fructose/D-Glucose in wine, beverages, foodstuffs and other materials. Measurements can be performed separately or combined.  Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.

Find more of our monosaccharide and oligosaccharide assay kits.

Advantages

• ”Ready to use” liquid stable formulation – no reconstitution needed
• PVP incorporated to prevent tannin inhibition
• Standard included 
• Suitable for manual and auto-analyzer formats
• Quick Reference Guide available
• Mega-Calc™ software tool is available for hassle-free raw data processing

The D-Fructose/D-Glucose (Liquid Ready™) assay kit is a method for the specific measurement and analysis of D-Fructose/D-Glucose in wine, beverages, foodstuffs and other materials. Measurements can be performed separately or combined. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.

Introduction

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Details

Specifications

Features	Specifications
Main Functions	Recover DNA fragments >100bp from agarose gel(<0.5g), purification of DNA from PCR, enzymatic reaction solution or crude gDNA
Applications	PCR, NGS, labeling, ligation and enzyme digestion, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Agarose gel, PCR products, enzyme products
Sample amount	Agarose gel: ≤500mg
Recovery	≥80%
Elution volume	≥15μl
Time per run	≤20 minutes（1-24 samples）
Liquid carrying volume per column	800µl
Binding yield of column	35µg

Principle

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

• High recovery efficiency – ≥80% DNA recovery
• General – recover DNA from gel or enzyme-driven reaction solutions such as PCR
• Fast – isolation can be completed in 10-15 minutes by column gel method
• Great cost-effectiveness performance

Kit Contents

Contents	D211102	D211103
Purification Times	100 Preps	250 Preps
Buffer GDP	120 ml	250 ml
Buffer DW2	50 ml	2 x 50 ml
Elution Buffer	20 ml	30 ml
HiPure DNA Mini Columns II	100	250
2 ml Collection Tubes	100	250

Storage and Stability

The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.

Experiment Data

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.