Opentrons OT-2 Filter Tips, 20µL. Optimized for volumes 1µL to 20µL. Clear Tips, Polypropylene tip, Polyethylene Filter Mesh, RNase-/DNase-free, Nonpyrogenic, Protease free. Pre-sterilized with electron beam irradiation. Not Autoclavable.
Compatible with: Opentrons 8-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN2 P20 Electronic Pipettes, Opentrons 8-Channel GEN1 P10 Electronic Pipettes
Opentrons OT-2 Filter Tips, 20µL. Optimized for volumes 1µL to 20µL. Clear Tips, Polypropylene tip, Polyethylene Filter Mesh, RNase-/DNase-free, Nonpyrogenic, Protease free. Pre-sterilized with electron beam irradiation. Not Autoclavable.
Compatible with: Opentrons 8-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN2 P20 Electronic Pipettes, Opentrons 8-Channel GEN1 P10 Electronic Pipettes
MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
This method is suitable for small quantities of tissue (<100mg) and cells (<5 X106), and large quantities of tissue (up to 1g) and cells (<108), of human, animal, plant, or bacterial origin. The simplicity of the MagZol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA prepared in this manner can be used for Northern blot analysis, dot blot hybridization, poly(A) + selection, in vitro translation, RNase protection assay, and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.
Specifications
Features | Specifications |
Main Functions | RNA isolation solvent (substitution for Trizol/Qiazol reagent) |
Applications | RT-PCR, Northern hybridization, poly (a) enrichment, etc. |
Purification technology | Acid phenol guanidine isothiocyanate |
Process method | Manual (centrifugation) |
Sample type | Animal tissue, plant tissue, adherent cells, suspended cells, bacteria, trace fungi, etc. |
Sample amount | Flexible |
Elution volume | Variation with sample size |
Time per run | Variation with sample size |
Storage and Stability
MagZol Reagent should be stored at 2-8°C upon arrival and is stable for at least 24 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance.
Experiment Data
MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
Bis-propargyl-PEG9 is crosslinking reagent containing two propargyl groups. These functional groups enable the formation of triazole linkages with azide-bearing compounds with the presence of copper catalyst. The hydrophilicity of the molecule is improved because of the PEG chain. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-propargyl-PEG9 is crosslinking reagent containing two propargyl groups. These functional groups enable the formation of triazole linkages with azide-bearing compounds with the presence of copper catalyst. The hydrophilicity of the molecule is improved because of the PEG chain. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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