Norgen’s Urine DNA Isolation Kit for Exfoliated Cells or Bacteria is designed for the rapid isolation of either: 1) human genomic DNA from exfoliated cells that have been shed into the urine from the urinary tract; or 2) bacterial genomic DNA from urine samples. The kit allows for the isolation of DNA from 1 to 50 mL of urine. The genomic DNA isolated from exfoliated cells can be used in a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers. Bacterial genomic DNA from both human urine samples and urine samples from animals can be isolated with this kit in order to study the levels and types of bacteria that are present. The kit allows for the isolation of genomic DNA from both Gram negative and Gram positive bacteria, including E. coli, Proteus spp., Klebsiella spp., Enterobacter spp., Serratia spp., Pseudomonas spp, Clostridial ssp. and Leptospirosis spp.Chlamydia trachomatis and Neisseria gonorrhoeae.
Typical yields of human genomic DNA from exfoliated cells will vary depending on the cell density of the urine sample, which is affected by a number of factors including health, diet and sex of the individual donating the urine. Typical yields of bacterial genomic DNA will vary depending on the urine sample and the bacterial species, if any, present in the urine. Healthy humans generally have < 10, 000 CFU of bacteria per mL of urine, and this kit is sufficiently sensitive to isolate and detect DNA from even this minimal amount of bacteria. The genomic DNA purified with this kit is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications such as PCR, qPCR and Southern Blot analysis.
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| Kit Specifications | |
| Minimum Urine Input | 1 mL |
| Maximum Urine Input | 50 mL |
| Time to Complete 10 Purifications | 10 minutes (Plus a 30 minute incubation – Bacteria) (Plus a 15 minute incubation – Exfoliated) |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.
| Component | Cat. 47050 (50 preps) |
|---|---|
| Resuspension Solution A | 20 mL |
| Lysis Buffer B | 40 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 8 mL |
| Proteinase K in Storage Buffer | 1.2 mL |
| Micro Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Features
1. Brushless frequency motor, in great torque, free maintenance, no power pollution, quick in speed up and down.
2. 7” LCD Touch screen display which indicates the program, rotor No, speed, time, RCF and temperature.
3. CFC-free refrigerant, pre-cooling function ,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control.
4. Microprocessor control , 100 programs in store, 10 kinds of acceleration and deceleration for your choice.
5. Over speed and imbalance protection.
6. Speed, RCF, time, temperature can be edited during running.
7. High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect sleeve, 3 tiers protection, safe and reliable.
8. Automatic door open & close, as well as anti-pinch function (Optional).
CDL7M Technical Parameter：
| Max. Speed | 7000rpm |
| Max. RCF | 11650×g |
| Max. Capacity | 6×2400ml (12*500ml blood bag） |
| Time Range | 1~99h59min59s |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 65 |
| Temperature | -20 ~ 40℃ |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | ±1℃ |
| Voltage(V/Hz) | AC 220V/380V 50HZ/60HZ |
| Size (W x D x Hmm) | 940×890×1000mm |
| Net Weight(Kg) | 570KG |
| Certificates | CE,ISO & Calibration report are available |
Special rotor for blood bag separation
| Order No. | Rotor No. | Max Speed (rpm) | Max Volume(ml) | Max. RCF（×g） |
| 05202 | Swing Rotor | 4000 | 6×2x1000ml | 5210 |
CDL7M touch screen with special programs for blood bag separation, can get good performance for platelet, red cell, all parts separation of blood. 6x2x1000ml bucket suitable for blood bag separation. Also with automatical lid lock open and close function, also wind shield can open along with door open.
Model:
CDL7M
Brand:
Yingtai
Code:
CDL7M blood bank centrifug