125mL HDPE Narrow Mouth Bottle
Perfect for sample storage
Development and product launch
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasmausually as short fragments, <1000bp(DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. The extracted products can be used for clinical in vitro detection.
Specifications
Features | Specifications |
Main Functions | Isolation circulating DNA from 1-5ml plasma, serum, bodyfluids by spin |
Applications | qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation) |
Sample type | Serum, plasma and other cell-free fluid samples |
Sample amount | 1-5ml |
Elution volume | ≥50μl |
Time per run | ≤70 minutes |
Liquid carrying volume per column | 4ml |
Binding yield of column | 1mg |
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption plate and filter column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10 Mm Tris,pH 8.0).
Kit Contents
Contents | D318203D |
Purification Times | 50 Preps |
Buffer ACL | 250 ml |
Buffer ACB* | 300 ml |
Buffer DCW1* | 22 ml |
Buffer DCW2* | 10 ml |
Proteinase K | 540 mg |
Protease Dissolve Buffer | 30 ml |
Carrier RNA | 110 μg |
Nuclease Free Water | 20 ml |
HiPure CFDNA Mini Columns | 50 |
2 ml Collection Tubes | 100 |
Extender Tubes | 50 |
Sealing O-Ringes | 50 |
50ml Collection Tube | 50 |
Support Tube | 50 |
Storage and Stability
Proteinase K, Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can bestored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasmausually as short fragments,
The Saxitoxin (PSP) ELISA Kit is a competitive ELISA for the quantitative analysis of saxitoxin in shellfish samples. This kit can be used for rapid test of Saxition in samples such as mussels and lobster tomalley.
The Saxitoxin (PSP) ELISA Kit uses a polyclonal antibody that binds both saxitoxin and a Saxitoxin-enzyme conjugate. Saxitoxin in the sample competes with the Saxitoxin-enzyme conjugate for a limited number of antibody binding sites.
Drinking Water Thresholds:
Do not Drink – 0.2 μg/L for all
Do not Use – 3 μg/L
96-well microtiter plate (12 test strips of 8 wells)
Standards: 0 | 0.02 | 0.08 | 0.32 ppb
Incubation Time: 60 Minutes.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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