Description
The ExcelTaq™ Hot Start II DNA Polymerase is a mixture of an aptamer-based inhibitor and a recombinant thermo-stable Taq DNA polymerase designed for preventing or minimizing non-specific DNA amplification in PCR reaction. The inactivation of polymerase is achieved by a reversible binding of the aptamer to the polymerase at temperatures below 45°C. The aptamer inhibitor releases polymerase during normal PCR cycling. The aptamer-based inhibition omits the time-consuming initial activation step required by chemically modified or antibody-based hot start polymerases.
The high specificity and sensitivity of ExcelTaq™ Hot Start II DNA Polymerase allows sensitive detection from limited amount of DNA templates, such as 1 pg of cDNA or 1 fg of plasmid DNA. With a high DNA synthesis rate and high thermo-stability, the ExcelTaq™ Hot Start II DNA Polymerase allows reactions to be set up at room temperature and is suitable for common and specialized PCR applications
Features
Applications
Storage
-20°C for 24 months
The ExcelTaq™ Hot Start II DNA Polymerase is a mixture of an aptamer-based inhibitor and a recombinant thermo-stable Taq DNA polymerase designed for preventing or minimizing non-specific DNA amplification in PCR reaction. The inactivation of polymerase is achieved by a reversible binding of the aptamer to the polymerase at temperatures below 45°C. The aptamer inhibitor releases polymerase during normal PCR cycling. The aptamer-based inhibition omits the time-consuming initial activation step required by chemically modified or antibody-based hot start polymerases.
The high specificity and sensitivity of ExcelTaq™ Hot Start II DNA Polymerase allows sensitive detection from limited amount of DNA templates, such as 1 pg of cDNA or 1 fg of plasmid DNA. With a high DNA synthesis rate and high thermo-stability, the ExcelTaq™ Hot Start II DNA Polymerase allows reactions to be set up at room temperature and is suitable for common and specialized PCR applications
More than 70 types of human papillomavirus (HPV) have been identified, and are generally classified as high-risk or low-risk depending on their relationship or lack of relationship with cancer and high-grade cervical intraepithelial neoplasia (CIN 2-3). HPV viruses are predominantly sexually transmitted and high-risk HPV types are a major risk factor for development of cervical cancer. HPV 16 has been considered as a high-risk cancer associated HPV type. The low-risk HPV type 6 has been associated with the presence of genital warts. There are many other low-risk HPV types that are not associated with genital warts or cervical cancer. Until now, HPV cannot be cultured in vitro, and immunological tests are inadequate to determine the presence of HPV cervical infection. On the other hand, biopsies can be analyzed by nucleic acid hybridization to directly detect the presence of HPV DNA.
HPV 6/16 TaqMan PCR Kit, 100 reactions
HPV 6/16 TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | TaqMan Probe Cat. TM42050 (100 preps) | TaqMan Probe/Primer and Control Set Cat. TM42010 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 µL | – |
| HPV 6/16 Primer & Probe Mix | 280 µL | 280 µL |
| HPV 6/16 Positive Control | 150 µL | 150 µL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
The TL 24 series of biological Microscope
TL 24 series biological microscope is equipped with 195 achromatic objective and large field eyepiece, with the characteristics of beautiful appearance, clear imaging, broad vision, convenient operation and so on, etc. It can be widely used in biology, medicine, industry, agriculture and other fields, and is an ideal instrument for medical, teaching, scientific research and other units
Details of TL24 series biological microscope
* Optical system: equipped with limited far flat field optical system, complete machine mildew prevention design and large field eyepiece, with beautiful appearance, clear imaging, broad vision, convenient operation and other characteristics, can be widely used in biology, medicine, industry, agriculture and other fields, is the ideal instrument for medical, teaching, scientific research and other units
* Observation head device: the cylinder can rotate 360 degrees, the displacement of the center of the mirror is 0.06mm; 30 tilting; the hinge double observation (the left and right systems are 2.0% different; the lens end position is 0.1mm at zero vision) and the third party connection port, pupil distance adjustment range of 55-75mm, fixed observation tube head is internal hexagon screw or select portable screw.
* Eyepiece configuration: random eyepiece WF10X / 18mm; the magnification accuracy of eyepiece shall not exceed ± 2%. There is a card slot on the eyepiece tube to lock and position the eyepiece to prevent falling. Binocular vision is adjustable, making binocular observation easier;
* Objective: Broadband coated ED-N195 pure copper achromatic objective; objective magnification accuracy not exceeding ± 5%; 4X, 10X, 40X (spring), 100X (spring, oil); objective error 10-40 Afocal 0.06mm; 40-100 Afocal 0.03mm
* Loading platform: triangular rail XY composite mechanical moving loading platform; 0.005 lateral to 5N horizontal force, corrosion resistance and wear resistance. Rectangular, size 110X120mm, stroke 7030mm, minimum read value 0.1 mm.
Specification
| Specification configuration | TL24A | Binocular observation head, 45° tilt, 360 degrees can be rotated, pupil distance can be adjusted, adjustment range 50-75mm |
| TL24B | Three-eye observation head, 45° tilt, 360 degrees can be rotated, pupil distance can be adjusted, adjustment range 50-75mm. | |
| TL24M | Three-eye viewing head, equipped with 5 million pixel imaging system CMOS camera | |
| TL24P | Three-eye viewing head, equipped with 10.1 inch integrated flat panel display | |
| Eyepiece | Wide-angle eyepiece WF10X,field of view 18mm,optional WF16X,WF25X | |
| Converter | Quadruple Revolving Converter | |
| 0bjective | 195mm achromatic objective,4X,10X,40X,100X(oil) | |
| Condenser | ABBE N.A.1.25Condenser with Iris Diaphragm &Filter | |
| Stage | Double Layer Mechanical Stage,Size110mmx120mm,Moving Range :70mmx30mm | |
| Focusing | Crude motion, micromotion and coaxial focal adjustment system with a minimum focal range of 0.02mm Coarse and Fine Focusing | |
| Light source | 1W LED,with Rechargeable Battery,Type C USB cable | |
| Attachments | Dust bag,Type C power cord | |
TL 24 series biological microscope is equipped with 195 achromatic objective and large field eyepiece, with the characteristics of beautiful appearance, clear imaging, broad vision, convenient operation and so on, etc. It can be widely used in biology, medicine, industry, agriculture and other fields, and is an ideal instrument for medical, teaching, scientific research and other units