Lactulose Assay Kit
Content: | 50 assays per kit |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | Lactulose |
Assay Format: | Spectrophotometer |
Detection Method: | Absorbance |
Wavelength (nm): | 340 |
Signal Response: | Increase |
Linear Range: | 0.65 to 65 µg of lactulose per “Enzymatic Determination Reaction |
Limit of Detection: | 4.8 mg/L |
Reaction Time (min): | ~ 120 min |
Application examples: | Milk, dairy products and foods containing milk. |
Method recognition: | Novel method |
The Lactulose Assay Kit is suitable for the specific, rapid and sensitive measurement and analysis of lactulose in milk and milk-based samples. Reagents included in this kit may also be prepared for use in the procedure described by ISO Method 11285:2004.
Check out our other monosaccharide and disaccharide assay kits.
Advantages
The Lactulose Assay Kit is suitable for the specific, rapid and sensitive measurement and analysis of lactulose in milk and milk-based samples. Reagents included in this kit may also be prepared for use in the procedure described by ISO Method 11285:2004.
MagPure Seed DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and seed. This kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo. Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridizationand transgenosis detection.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from 50-100 mg easy-grinded plant (tender leaf) and seed |
Applications | PCR, transgene detection, fluorescence quantitative PCR, southern blot, SNP site analysis, etc. |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | Conventional economic plant samples |
Sample amount | 50-100mg |
Elution volume | ≥50μl |
Time per run | ≤60 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Kit Contents
Contents | D635201 | D635202 | D635203 |
Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
MagPure Particles | 1.7 ml | 4 ml | 18 ml |
Buffer SOL | 40 ml | 90 ml | 500 ml |
Buffer SDS | 4 ml | 9 ml | 50 ml |
Buffer MPB | 40 ml | 70 ml | 350 ml |
Buffer GW1 | 26 ml | 53 ml | 220 ml |
Elution Buffer | 10 ml | 30 ml | 120 ml |
Storage and Stability
MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
MagPure Seed DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and seed. This kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo. Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridizationand transgenosis detection.
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