This kit provides a fast and simple spin column procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000), as well as fresh saliva samples.
Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.
Saliva represents an excellent non-invasive alternative to blood collection. Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples.
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Kit Specifications | |
Maximum Saliva Input | 0.5 mL preserved saliva 0.25 mL fresh saliva |
Average Yield from 0.25 mL of Saliva* | 3 – 7 μg |
Average Purity (OD260/280) | 1.7 – 2.1 |
Time to Complete 10 Purifications | 30 minutes |
* Average yield will depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
Component | Cat. RU45400 (50 preps) |
---|---|
Lysis Buffer F | 30 mL |
Proteinase K in Storage Buffer | 1.2 mL |
Binding Buffer B | 12 mL |
Wash Solution A | 18 mL |
Elution Buffer B | 15 mL |
Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |
K-GAMINE
SKU: 700004290
50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)
Content: | 50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser) |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | N-Acetyl-D-Glucosamine, D-Glucosamine, D-Glucosamine sulphate |
Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
Detection Method: | Absorbance |
Wavelength (nm): | 340 |
Signal Response: | Increase |
Linear Range: | 4 to 80 µg of glucosamine per assay |
Limit of Detection: | 1.33 mg/L |
Reaction Time (min): | ~ 8 min |
Application examples: | Food supplements, food products and beverages. |
Method recognition: | Novel method |
The D-Glucosamine test kit is a high purity reagent for the measurement and analysis of D-glucosamine in neutraceutical/food products. This kit can also be used to measure N-Acetyl-D-Glucosamine and D-Glucosamine sulphate.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
See our complete list of monosaccharide and disaccharide assay kits.
Advantages
The D-Glucosamine test kit is a high purity reagent for the measurement and analysis of D-glucosamine in neutraceutical/food products. This kit can also be used to measure N-Acetyl-D-Glucosamine and D-Glucosamine sulphate.
HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.
Specifications
Features | Specifications |
Main Functions | Recover DNA fragments between 20bp-20kbp from crude DNA and enzymatic reaction solution |
Applications | PCR, sequencing, labeling reactions, ligations and restriction digestion, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Crude DNA, enzymatic reaction solution |
Sample amount | <150μl |
Recovery | 80% |
Elution volume | ≥15μl |
Time per run | ≤10 minutes |
Liquid carrying volume per column | 800µl |
Binding yield of column | 20µg |
The HiPure system uses a simple bind-wash-elute procedure. Gelslices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
Contents | D214102 | D214103 |
Purification Times | 100 Preps | 250 Preps |
Buffer GWP | 30 ml | 60 ml |
Buffer DW2 | 20 ml | 50 ml |
Elution Buffer | 30 ml | 60 ml |
HiPure DNA Mini Columns I | 50 | 250 |
2 ml Collection Tubes | 50 | 250 |
Storage and Stability
HiPure DNA Clean Up Kit components are guaranteed for at least one year when stored at room temperature. If any precipitates form in the buffers, warm at 37℃ to dissolve.
HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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