10 Layer Cell Factory Double Narrow Mouth
Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
Specifications: 1 layer 2 layers, 5 layers, 10 layers and 40 layers.
PRODUCT FEATURES
*The product is made of medical grade USP CLASS VI polymer polystyrene
*The product assembled with ultrasonic welded technology
of one 10-layer Cell Factory unit is equivalent to the area
of 36 T-175 flasks
Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
Specifications: 1 layer 2 layers, 5 layers, 10 layers and 40 layers.
This kit features a rapid, high-throughput 96-well format for isolating high yield and high quality DNA from saliva. The purified saliva DNA is compatible with any number of downstream applications including PCR, qPCR, Southern Blot analysis, sequencing, microarray analysis and more. This kit is also compatible with Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000). It is fully compatible to both vacuum and centrifugation protocols.
Background
Human genomic DNA found in saliva can be used in various applications in diagnostics including the detection of biomarkers to diagnose a disease, follow the disease’s progress or monitor the effects of a particular treatment. Isolation of DNA from saliva for diagnostics has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive.
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| Kit Specifications | |
| Binding Capactity Per Well | 50 μg |
| Maximum Loading Volume Per Well | 600 μL |
| Size of DNA Purified | All sizes |
| Time to Complete 96 Purifications | 40 minutes |
Storage Conditions and Product Stability
The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at room temperature or 4°C. All other solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.
| Component | Cat. RU35200 (192 preps) |
|---|---|
| Lysis Buffer F | 100 mL |
| Proteinase K in Storage Buffer | 4 mL |
| Binding Buffer B | 40 mL |
| Wash Solution A | 2 x 38 mL |
| Elution Buffer B | 30 mL |
| 96-Well Plate | 2 |
| 96-Well Collection Plate | 2 |
| Adhesive Tape | 4 |
| 96-Well Elution Plate | 2 |
| Product Insert | 1 |
Norgen’s EXTRAClean Urine Exosome Purification and RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of RNA from different urine sample volumes ranging from 250 μL to 1 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, NGS application, Northern blotting, RNase protection and primer extension, and expression array assays.
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| Minimum Urine Input | 2 mL |
| Maximum Urine Input | 10 mL |
| Size of Exosomes Purified | 40 nm – 150 nm |
| Size of RNA Purified | All sizes, including miRNA and small RNA (< 200 nt) |
| Elution Volume | 50-100 μL |
| Time to Complete 10 Purifications | 35-40 minutes |
| Average Yields | Variable depending on specimen |
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.