These kits are designed to clean-up and concentrate RNA (including miRNA) from TRIzol® and TRI Reagent®, enzymatic reactions, in vitro transcription, labelling reactions, etc. These are robust kits for all clean-up and concentration purposes for up to 35 µg of RNA in solution. The purified RNA is of the highest purity and integrity, and can be used in a number of downstream applications. These kits purify all sizes of RNA, from large mRNA and ribosomal RNA down to miRNA, siRNA and lncRNA.
Norgen’s RNA Clean-Up and Concentration Kit provides a rapid method for the purification, cleanup and concentration of up to 35 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 20 µL. Complete 10 purifications in 20 minutes.
Norgen’s RNA Clean-Up and Concentration 96-Well Kit provides a rapid method for the purification, cleanup and concentration of up to 50 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 75 µL. Complete 10 purifications in 30 minutes.
Norgen’s RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration of up to 10 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. This kit is made for eluting RNA in smaller volumes of 8 µL for all types of downstream applications, for the highest concentration of RNA sample. Complete 10 purifications in 20 minutes.
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| Kit Specifications (Spin Column) | |
| Maximum Column Binding Capacity | 35 μg |
| Size of RNA Purified | All sizes, including small RNA (<200 nt) |
| Maximum Amount of Starting Material | 35 μg of RNA |
| Minimum Elution Volume | 20 μL |
| Time to Complete 10 Purifications | 20 minutes |
| Average Recovery | ≥ 90% |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 23600 (50 preps) | Cat. 43200 (100 preps) | Cat. 25100 (192 preps) | Cat. 61000 (50 preps) |
|---|---|---|---|---|
| Buffer RL | 40 mL | 2 x 40 mL | 2 x 40 mL | 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL | 2 x 38 mL | 38 mL |
| Elution Solution A | 6 mL | 2 x 6 mL | 2 x 20 mL | 6 mL |
| Column Activation Solution | – | – | – | 30 mL |
| Micro Spin Columns | 50 | 100 | – | – |
| Micro-Elute RNA Spin Columns | – | – | – | 50 |
| 96-Well Plate | – | – | 2 | – |
| Adhesive Tape | – | – | 4 | – |
| Collection Tubes | 50 | 100 | – | 50 |
| 96-Well Collection Plate | – | – | 2 | – |
| Elution Tubes (1.7 mL) | 50 | 100 | – | 50 |
| 96-Well Elution Plate | – | – | 2 | – |
| Product Insert | 1 | 1 | 1 | 1 |
The kit was developed for construction of high quality libraries for next generation sequencing (illumina and MGI Platforms). The kit needs double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library preparation, and is compatible with DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.). Library multiplexing is possible with different types of indexes.
The kit was optimized for next generation sequencing (NGS) library preparation with different types of samples. Most of DNA library preparation requires the ligation of sheared DNA fragments to library adaptors and the DNA library preparation is closely related to the quality of NGS data. With BioDynami’s unique DNA library preparation technologies, the fast and simple kit allows high quality NGS library preparation to be completed in 1.5 hours with only 10 minutes of hands-on time.
Some genomic regions are very difficult to be covered evenly and usually result in very low coverage rate or gap in these regions.
Typical difficult regions are:
• with high GC contents
• have secondary structures: mainly due to repeat sequences
• the worst cases: have both high GC contents and repeated sequences.
Example: human TERT gene is one of the most difficult regions as shown above. NGS data showed that BioDynami kit has the best performance to cover the extremely difficult human TERT gene region.
Limited GC bias across whole genome
Three index types are available for the illumina platform kits:
Non-index (illumina Cat.# 30009): Libraries do not have index.
Index (illumina Cat.# 30021): A unique barcode sequence with 6 bases has been included in each of the index primers. RNA Sequencing library multiplexing is possible with up to 48 samples. Index information can be downloaded here.
Unique dual index (illumina Cat.# 30023): RNA Sequencing library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a 4-Base Difference Index System. The system can generate indexes with at least 4 bases different from others in the 8-base indexing region. the unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors. Index information can be downloaded here.
Indexes are available for the MGI platform kits (Cat.# 34021).
The kit was developed for construction of high quality libraries for next generation sequencing (illumina and MGI Platforms). The kit needs double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library preparation, and is compatible with DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.). Library multiplexing is possible with different types of indexes.
Lupus test – La antigen lateral flow serology test. Rapidly detect antibodies targeting the human La autoantigen. 10 tests per kit.
About La: The La autoantigen has been implicated to be important for many different cellular processes including RNP maturation, internal ribosome-entry-site-mediated translation, mRNA stabilization, and transcription termination. La binds to the poly U tracts of polymerase III precursor RNAs and certain polymerase II transcribed RNAs.
Attogene La antigen serology test can be used to detect autoantibodies targeting the La Protein found in human Sera and differentiate IgG and IgM.
