6×50ml Angle rotor 

• Ready to Use  Streptavidin Europium Chelate Microspheres
• Specifically Designed Running Buffer

Description

Europium Chelate Microspheres Nanoparticle Streptavidin Conjugate for Lateral Flow

Our Streptavidin Europium Chelate Microspheres conjugate is manufactured using special conjugation technology and functonally tested by lateral flow. We have coated our high quality nanoparticles with a proprietary surface coat that covalently binds the biotin forming ultra stable conjugates. The resulting Streptavidin Europium Chelate Microspheres  can irreversibly bind biotin.

Europium dyed polystyrene beads conjugated to streptavidin

250ul of a 1mg/ml stock ready to use for Lateral Flow

50mL Lateral FLow Running Buffer designed for Streptavidin Europium Chelate Microspheres 

Inquire for Larger Volumes: sales@attogene.com

Excitation max: 365nm
Emission max: 610nm

Europium Chelate Microspheres Nanoparticle Streptavidin Conjugate for Lateral Flow

Our Streptavidin Europium Chelate Microspheres conjugate is manufactured using special conjugation technology and functonally tested by lateral flow. We have coated our high quality nanoparticles with a proprietary surface coat that covalently binds the biotin forming ultra stable conjugates. The resulting Streptavidin Europium Chelate Microspheres can irreversibly bind biotin.

Overview

• Excellent separation and purification of cytoplasmic and nuclear RNA
• Convenient and fast spin column format
• High quality and yield of RNA
• Isolate full diversity of RNA (including microRNA) without phenol
• Purified RNA is ready for any application including RT-PCR, qRT-PCR, RNA-Seq, arrays and more
• Cytoplasmic RNA is free of DNA and ready for direct use in RT-PCR/qRT-PCR
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

This kit provides a rapid method for the isolation and purification of both cytoplasmic and nuclear RNA from cultured animal cells and small tissue samples. The kit can be used to isolate all sizes of RNA from the cytoplasmic and nuclear RNA fractions, including all small RNA species without any requirement for phenol.   Included in the kit are sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and 25 nuclear RNA preparations.   Ten samples can be processed in approximately 45 minutes.  This kit is also available in a 100 prep size.    

Background

In certain circumstances it is desirable to be able to isolate fractionated RNA as opposed to total RNA. For example, it may be preferable to isolate only mature, cytoplasmic RNA for some studies on expression profiling. Alternatively it may be desirable to isolate nuclear RNA in order to investigate and study pre-processed (non-spliced) RNA. Furthermore, this kit can be used to isolate RNA for downstream applications where it is necessary to avoid DNA contamination, since the cytoplasmic fraction has been shown to be free of all traces of genomic DNA. 

Details

Supporting Data

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Kit Specifications
Maximum Column Binding Capacity	Up to 50 µg RNA
Maximum Column Loading Volume	650 µL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Time to Complete 10 Purifications	45 minutes
RNA Yield HeLa (1 x 106) – Cytoplasmic RNA HeLa (1 x 106) – Nuclear RNA	15 µg ≤ 3.5 µg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 21000 (50 preps)	Cat. 37400 (100 preps)
Lysis Buffer J	20 mL	2 x 20 mL
Buffer SK	40 mL	2 x 40 mL
Wash Solution A	38 mL	2 x 38 mL
Elution Buffer E	6 mL	2 x 6 mL
Mini Spin Columns	50	100
Collection Tubes	50	100
Elution Tubes (1.7 mL)	50	100
Product Insert	1	1

Cytoprep-1 bench top cytology centrifuge

Features:

1.Widely used in the field of biochemistry、biological products、pharmaceutical factory and laboratory.

2. Brushless DC motor for model Cytoprep-1, free maintenance, no powder pollution, quick in speed up and down.

3. Smooth in operation, low noise and small vibration.

4. Frame is 3 tiers protection steel jacket, and with the stainless steel chamber, inside protective steel lagging which is simple-compact, light weight.

5. Microprocessor control, it can control rotate speed, and relative RCF, digital display which indicates the speed, time, RCF.

6. Automatically electric lid lock, super speed、over temperature protection and imbalance protection.

7. Power: AC 110V/220V, 50/60HZ, speed range:0-2800rpm, speed accuracy:± 20rpm

8. Emergency locks under the bottom of the centrifuge which help you open the lid when power off.

9. The centrifuge body is made of high-quality steel, safe and reliable.

Cytoprep-1 Cytology Technical Parameters:

Max speed：	4000rpm	Max RCF：	2170×g
Max volume	6×30ml	Noise：	≤55dBA
Timer	0～99min	Net weight：	28 KG
Dimension(HxDxW)：	280×528×370mm	Power supply	AC 220V 50HZ 2A
Speed accuracy：	±20rpm	Package	carton box or wooden box