Introduction

MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 100-150mg stool samples
Applications	PCR, southern blot and enzyme digestion, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Stool
Sample amount	100-150 mg
Elution volume
Time per run

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• Fast – several samples can be extracted in 60 minutes (after digestion)
• High purity – unique adsorbent can completely remove inhibitory factors
• High recovery – DNA can be recovered at the level of PG

Kit Contents

Contents	D6364
Purification Times	400 Preps
MagPure Particles N	14 ml
2ml Bead Tubes	400
PVP-10	6 g
RNase A	75 mg
Proteinase K	180 mg
Protease Dissolve Buffer	20 ml
Buffer ATL	300 ml
Buffer PCI	300 ml
Buffer MLE	180 ml
Buffer GW1*	132 ml
Elution Buffer	60 ml

Storage and Stability

MagPure Particles, RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.

Propargyl-PEG5-NHS ester has a propargyl group and an NHS ester group. The terminal NHS ester and is an amine reactive moeity which is useful for derivatizing peptides, antibodies, amine coated surfaces etc. The propargyl group can participate in Click Chemistry reactions with azide compounds, copper is needed for catalyzation. The PEG units can enhance the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG5-NHS ester has a propargyl group and an NHS ester group. The terminal NHS ester and is an amine reactive moeity which is useful for derivatizing peptides, antibodies, amine coated surfaces etc. The propargyl group can participate in Click Chemistry reactions with azide compounds, copper is needed for catalyzation. The PEG units can enhance the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG8-NHS ester is an amine reactive compound with an alkyne group. The alkyne group reacts with azide-containing biomolecules in Click Chemistry reactions, copper is needed as a catalyst. The hydrophilic PEG units helps the compound to have better solubility in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG8-NHS ester is an amine reactive compound with an alkyne group. The alkyne group reacts with azide-containing biomolecules in Click Chemistry reactions, copper is needed as a catalyst. The hydrophilic PEG units helps the compound to have better solubility in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.