Introduction

This product speeds and simplifies nucleic acid size selection for fragment library preparation for next generation sequencing.

Details

Specifications

Features	Specifications
Main Functions	Selectively recover DNA from PCR products and enzymatic reaction solution （Replace Beckmen or agencourt SPRISelect）
Applications	Prepare DNA library for second generation sequencing
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	DNA products, restriction endonuclease systems, or other enzymatic reaction solution
Sample amount	Appropriate
Recovery	80%
Operation time	≤50 minutes

Principle

The MagSelect method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments 100bp and larger to paramagnetic beads. Excessprimers, nucleotides, salts, and enzymes can be removed using a simple washing procedure. The result is a more purified PCR product.

Advantages

• High recovery – up to 80%
• High throughput – using magnetic beads purification technology

Kit Contents

Contents	XP-5	XP-50	XP-500
MagSelect Beads	5 ml	50 ml	500 ml

Storage and Stability

MagSelect XP should be stored at 2-8°C upon arrival and is stable up to 18 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. Shake the reagent well before use. It should appear homogenous and consistent in color.

DO NOT FREEZE.

This product speeds and simplifies nucleic acid size selection for fragment library preparation for next generation sequencing.

Overview

• Detection kits for RSV-A
• Available in TaqMan format for analysis

Norgen’s Respiratory Syncytial Virus A (RSV-A) TaqMan RT-PCR Detection Kit is designed for the detection of RSV-A specific RNA in a real-time PCR based on the use of TaqMan technology. This kit is designed for research use only and not for use in diagnostic procedures. The detection of RSV-A specific RNA is based on TaqMan one-step RT-PCR providing a simple, reliable and rapid result for the detection of RSV-A infection. Norgen’s TaqMan RT-PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The RSV-A TaqMan RT-PCR Kit comprises Master Mix for the target and PCR control detection, Primer & Probe Mix, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagents.

RSV-A TaqMan RT-PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

RSV-A TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM34250 (100 preps)	Cat. TM34210 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
RSV-A Primer & Probe Mix	280 μL	280 μL
RSV-A Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1