The RNA Seq Library Prep Kit was developed for construction of high quality NGS libraries for next generation sequencing (illumina and MGI Platforms). RNA Sequencing is a very powerful tool to analyze transcriptome such as gene expression and transcription regulation, splicing characterization, mutation and variation detection etc. The kit needs purified RNA (example: rRNA depleted RNA or polyA mRNA) as input for library construction. Library multiplexing is possible with different types of indexes.
RNA Seq Library Prep Kit Workflow
Three index types are available for the illumina platform kits:
Non-index (illumina Cat.# 30055): Libraries do not have index.
Index (illumina Cat.# 30056): A unique barcode sequence with 6 bases has been included in each of the index primers. RNA Sequencing library multiplexing is possible with up to 48 samples. Index information can be downloaded here.
Unique dual index (illumina Cat.# 30057): RNA Sequencing library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a 4-Base Difference Index System. The system can generate indexes with at least 4 bases different from others in the 8-base indexing region. the unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors. Index information can be downloaded here.
Indexes are available for the MGI platform kits (Cat.# 34112).
Features
Comparison of the protocol time: BioDynami RNA Seq Library Prep Kit vs other vendors’ kits. Hands-on time and walk-away time were indicated.
Library size and distribution of BioDynami kit, 20 ng and 3 ng of polyA mRNA as input.
Comparison of library yield and duplication rate under the same condition: 20 ng and 3 ng of polyA mRNA were used as input. PCR cycle numbers were indicated.
Comparison of coverage of transcripts: 20 ng of polyA mRNA were used as input. BioDynami kit has more uniform coverage
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Norgen’s Plasma/Serum Circulating DNA Purification Kits (Slurry Format) provide efficient methods for the purification of fragmented free-circulating DNA from human plasma or serum. The kit is able to isolate all sizes of circulating DNA. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes. DNA can be isolated from either fresh or frozen samples using this kit. The kit will also isolate viral and bacterial DNA from plasma/serum. Typical yields of purified free-circulating DNA will vary depending on the input sample (1-100ng/mL circulating DNA in human plasma), with more concentrated samples tending to yield more free-circulating nucleic acids. The purified, inhibitor-free plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.
Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 50 μL to 400 μL. Preparation time for a single sample is less than 30 minutes.
Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 400 μL to 2 mL. Preparation time for a single sample is less than 45 minutes.
Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 2 mL to 10 mL. Preparation time for a single sample is less than 45 minutes.
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| Kit Specifications | |
| Minimum Plasma/Serum Input | 50 μL |
| Maximum Plasma/Serum Input | 400 μL |
| Minimum Elution Volume | 100 μL |
| Time to Complete Purifications | < 30 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 50600 (50 preps) | Cat. 51200 (20 preps) | Cat. 51300 (10 preps) |
|---|---|---|---|
| Lysis Buffer D | 65 mL | 125 mL | 3 x 125 mL |
| Slurry A1 | – | 6 mL | – |
| Slurry A2 | 12 mL | – | 12 mL |
| Binding Buffer B | 20 mL | 6 mL | 20 mL |
| Binding Buffer C | – | – | 30 mL |
| Wash Solution A | 2 x 38 mL | 2 x 38 mL | 3 x 38 mL |
| Elution Buffer B | 8 mL | 8 mL | 15 mL |
| Mini Filter Spin Columns | 50 | 20 | – |
| Midi Filter Spin Columns | – | – | 10 |
| Midi Collection and Elution Tubes | – | – | 20 |
| Collection Tubes | 50 | 40 | 10 |
| Spin Columns | – | 20 | 10 |
| Elution Tubes (1.7 mL) | 50 | 40 | 10 |
| Product Insert | 1 | 1 | 1 |