Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
The series of nucleic acid columns produced by Magen Biotech are based on carefully selected imported glass fiber membranes (GF/B, GF/D, GF/F). Columns production processes such as polypropylene injection molding materials, injection molding process, and downstream membrane packing and compression rings are strictly controlled. This is to ensure that the column has extremely high adsorption capacity and long-term stability. Compared with conventional products on the market, Magen’s columns are with varieties, and binding rate will not change when stored at room temperature for 4 years.
Specifications
Features | Specifications |
Recommended application | Small amounts of nucleic acid isolation, viral nucleicacid from cell free samples |
Preservation conditions | Room temperature |
Stability | Up to 4 years |
Filter membrane | High quality glass fiber filter GF/F, 3 layers |
Membrane aperture | 0.7μm |
Maximum binding yield of plasmid | 30 μg |
Maximum yield of alcohol mediated Binding | 200 μg |
Single liquid carrying capacity of column | 800 μl |
Minimum elution volume | 30 μl |
Withstand centrifugal force | 16,000 x g |
Centrifuge | Small high speed centrifuge (2ml) |
Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silicagel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
Ordering information
CAT.No. | Product Name | Package |
C13112 | HiPure Viral Mini Column I (3 x GF/F)with 2ml Collection Tubes | 1000/Bag |
Item No. | Product Name | Membrane type/number of layers | Collection tubes | Plasmid DNA binding capacity (Physical adsorption) | gDNA/RNA binding capacity (Alcohol-mediated adsorption) | Minimum Elution volume | Liquid volume capacity |
C13010 | HiPure DNA Nano Column | 2 layers GF/F | 2ml without cap | 5μg | 20μg | 10μl | 700μl |
C13011 | HiPure DNA Micro Column | 3 layers GF/F | 2ml without cap | 10μg | 50μg | 15μl | 700μl |
C13100 | HiPure DNA Mini Column I | 2 layers GF/B | 2ml without cap | 15μg | 100μg | 30μl | 700μl |
C13110 | HiPure DNA Mini Column II | 4 layers GF/B | 2ml without cap | 35μg | 200μg | 50μl | 800μl |
C13111 | HiPure RNA Mini Column | 3 layers GF/B | 2ml without cap | 30μg | 200μg | 30μl | 800μl |
C13112 | HiPure Viral Mini Column | 3 layers GF/F | 2ml without cap | 30μg | 200μg | 30μl | 800μl |
C13113 | HiPure CFDNA Mini Column | 3 layers GF/F,1 layer GF/B | 2ml without cap | 30μg | 200μg | 30μl | 800μl |
C13120 | HiPure DNA Midi Column | 4 layers GF/B | 15ml Centrifuge tube | 125μg | 1mg | 500μl | 4ml |
C13121 | HiPure DNA Midi Column III | 8 layers GF/B | 15ml Centrifuge tube | 250μg | 1mg | 500μl | 4ml |
C13122 | HiPure DNA Maxi Column | 4 layers GF/B | 50ml Centrifuge tube | 500μg | 5mg | 1000μl | 20ml |
C13123 | HiPure DNA Maxi Column III | 8 layers GF/B | 50ml Centrifuge tube | 1mg | 5mg | 1000μl | 20ml |
C13124 | HiPure DNA Maxi Column C | 8 layers GF/B | 50ml high speed Centrifuge tube | 1mg | 5mg | 700μl | 12ml |
C13130 | HiPure DNA Plate | 2 layers GF/F | 1.6ml Plate | 30μg | 100μg | 80μl | 900μl |
C13131 | HiPure gDNA Plate | 2 layers GF/B | 1.6ml Plate | 30μg | 100μg | 80μl | 900μl |
Note: GF/B pore size is for 1.0μM glass fiber membrane; GF/F pore size is for 0.7μm glass fiber membrane.
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
Propargyl-PEG4-CH2CO2H is a linker consisting of a propargyl group with a carboxylic acid group. The carboxylic acid can react with primary amine groups in the presence of activators such as HATU or EDC. The propargyl group can react with azide compounds in Click Chemistry; copper catalyst will be needed. The PEG units help the molecule to have better solubility in aqueous solution. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG4-CH2CO2H is a linker consisting of a propargyl group with a carboxylic acid group. The carboxylic acid can react with primary amine groups in the presence of activators such as HATU or EDC. The propargyl group can react with azide compounds in Click Chemistry; copper catalyst will be needed. The PEG units help the molecule to have better solubility in aqueous solution. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Attogene Universal Lateral Flow Assay Kits are a convenient ready-to-use kit for quick and cost-effective development of a lateral flow dipstick assay for detection of DNA and RNA products.
Formats (strep gold conjugate pad):
Detection of nucleic Acid (DNA or RNA) requires the use of a biotin and FAM/FITC-labelled primer during amplification. Test line: anti-biotin, Control Line: GAM
Multiplex detection of nucleic Acid (DNA or RNA) requires the use of a biotin, FITC/FITC and Dig labelled primers during amplification.: Test Line #1: anti FITC/FAM, Line #2: anti-Dig, Line #3 GAM.
Inquire about custom configurations: sales@attogene.com
Kit Components
Features & Benefits
Attogene Universal Lateral Flow Assay Kits are a convenient ready-to-use kit for quick and cost-effective development of a lateral flow dipstick assay for detection of DNA and RNA products.
Formats (strep gold conjugate pad):
Detection of nucleic Acid (DNA or RNA) requires the use of a biotin and FAM-labelled primer during amplification. Test line: anti-biotin, Control Line: GAM
Multiplex detection of nucleic Acid (DNA or RNA) requires the use of a biotin, FITC and Dig labelled primers during amplification.: Test Line #1: anti FITC/FAM, Line #2: anti-Dig, Line #3 Biotin.
Inquire about custom configurations: sales@attogene.com
Kit Components
Features & Benefits
100 Lateral Flow Dipsticks (4.5mm)
20 mL Assay running buffer
100 wells with support plate
Controls
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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