Bis-propargyl-PEG7 is a homobifunctional reagent with two propargyl groups that can be used to form triazole linkages with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The hydrophilic PEG units help improve the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Bis-propargyl-PEG7 is a homobifunctional reagent with two propargyl groups that can be used to form triazole linkages with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The hydrophilic PEG units help improve the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

Details

Specifications

Principle

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

Advantages

• Fast – several samples can be extracted in 20 minutes by column method
• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Safe – no phenol chloroform extraction required
• Sensitive – DNA/RNA can be recovered at the level of PG
• High yield – carrier RNA contained in the product maximize the recovery of trace nucleic acid

Kit Contents

Storage and Stability

This kit is shipped and stored at room temperature and is valid for 12 months.

Experiment Data

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

N-(Propargyl-PEG2)-DBCO-PEG3-NHS ester is a PEG linker with a terminal NHS ester to perform facile reactions with amine groups of molecules as well as a propargyl to react with azides to form a triazole. The DBCO can participate in copper-free Click Chemistry reactions.

N-(Propargyl-PEG2)-DBCO-PEG3-NHS ester is a PEG linker with a terminal NHS ester to perform facile reactions with amine groups of molecules as well as a propargyl to react with azides to form a triazole. The DBCO can participate in copper-free Click Chemistry reactions.