6x50ml Low Speed Angle Rotor 

Description 

Q-PAGE™ TGN (Tris-Glycine Novel) Precast Gels are ready-to-use acrylamide gels for SDS-PAGE running in Tris-Glycine buffer system. With unique formula, Q-PAGE™ TGN Precast Gels perform enhanced speed, better separation, and longer shelf life as compared with conventional Laemmli Tris-HCl gels. The protein migration patterns in Q-PAGE™ TGN series, however, are similar with typical Laemmli Tris-HCl gels, and thus Q-PAGE™ TGN Precast Gels are compatible to traditional SDS-PAGE and subsequent analyses. 

Q-PAGE™ TGN Precast Gels are available in gradient (4 to 15%) and fixed (10%) concentrations of polyacrylamide in 12- and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP4XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP5XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems. 

Key Features

• User-friendly gel cassette:
  • Numbered and framed wells for sample loading
  • Labeled warning sign and green tape as reminder
• Enhanced gel performance: 
  • Enhanced gel electrophoresis speed 
  • Better band separation, equivalent to commercially 4-20% TG gels  
  • Stable for shipping at ambient temperature
• Easy compatibility: 
  • Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
  • Compatible with most popular protein electrophoresis systems 

Storage and stability

Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.

Do not freeze Q-PAGE™ Precast Gels. Remove tape and comb before electrophoresis. 

Keep Q-PAGE™ Precast Gels flat during storage.

Q-PAGE™ TGN (Tris-Glycine Novel) Precast Gels are ready-to-use acrylamide gels for SDS-PAGE running in Tris-Glycine buffer system. With unique formula, Q-PAGE™ TGN Precast Gels perform enhanced speed, better separation, and longer shelf life as compared with conventional Laemmli Tris-HCl gels. The protein migration patterns in Q-PAGE™ TGN series, however, are similar with typical Laemmli Tris-HCl gels, and thus Q-PAGE™ TGN Precast Gels are compatible to traditional SDS-PAGE and subsequent analyses. 

Introduction

Intended Use

For selective enrichment culture of Listeria monocytogenes in food.

Principle and Interpretation

Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen sources, vitamins, amino acids, and growth factors; sodium chloride can maintain a balanced osmotic pressure; sodium dihydrogen phosphate and potassium dihydrogen phosphate as buffering agents; esculin are fermentable sugars; lithium chloride and other antibiotics can inhibit Gram negative bacteria and most Gram positive bacteria.

Formulation

Ingredients	/liter
Enzymatic digest of animal tissues　( Proteose peptone)	5.0g
Enzymatic digest of casein　( Tryptone)	5.0g
Meat extract	5.0g
Yeast extract	5.0g
Sodium chloride	20.0g
Disodium hydrogen phosphate dihydrate	12.0g
Potassium dihydrogen phosphate	1.35g
Aesculin	1.0g
Lithium chloride	3.0g
pH7.2±0.2 at 25°C

Preparation

Suspend 57.4g in 1 L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121℃ for 15 minutes. Cool to 45-50℃,aseptically add the contents of 1 vial of Fraser Selective Supplement (SR0120) to each 225 mL of base to prepare FB1 solution; Add one reagent (SR0130) to each100 mL of FB2 culture medium to prepare FB2 enrichment solution.

Quality Control

Cultural characteristics observed after incubation at 35-37°C for 46~50 hours

Quality control strains	Growth	Characteristics
Listeria monocytogenes ATCC19115 + Escherichia coli ATCC25922 + Enterococcus faecalis ATCC29212	>20 cfu  On PALCAM	Gray to black colony count with black halo
Escherichia coli ATCC25922	< 100 colonies on TSA	Inhibition
Enterococcus faecalis ATCC29212

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Revision

On June 14, 2024

References

ISO 11290

Intended Use For selective enrichment culture of Listeria monocytogenes in food. Principle and Interpretation Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen ……

Propargyl-PEG6-alcohol is alkyne linker that can react with azide compounds via copper catalyzed azide-alkyne Click Chemistry. The hydrophilic PEG8 spacer increases solubility in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG6-alcohol is alkyne linker that can react with azide compounds via copper catalyzed azide-alkyne Click Chemistry. The hydrophilic PEG8 spacer increases solubility in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.