6x50ml Low Speed Angle Rotor 

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Eleven discrete fragments ranging from 50 bp to 1000 bp
• Higher intensity reference band at 500 bp

The Norgen PCR Ranger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCR Ranger DNA Ladder contains eleven discrete fragments ranging from 50 bp to 1000 bp with a higher intensity reference band at 500 bp. This Ladder is ideal for assessment of a range of PCR product sizes.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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PCR Ranger 100 bp DNA Ladder (Cat# 11300) – 100 loads

Ladder Properties:
• Eleven discrete bands, ranging from 50 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference

Fragment	Size (bp)	Mass (ng)
1	1000	91
2	900	66
3	800	61
4	700	51
5	600	43
6	500	70
7	400	28
8	300	23
9	200	30
10	100	22
11	50	15

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 
Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Overview

• Detection kits for Streptococcus agalactiae
• Available in TaqMan format for analysis

Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the udder is mainly caused by infection of various bacteria. One of such mastitis bacteria, Streptococcus agalactiae, is highly infectious and causes mainly subclinical infections, which are not identified by the herdsman. As a result, S. agalactiae can spread widely within a herd, causing immediate loss due to reduced milk. S. agalactiae is a gram-positive bacteria belonging to the Group B streptococci. Traditional cultural identification of S. agalactiae is based on S. agalactiae being beta-hemolytic as well as presence of group B Lancefield antigen and by its ability to hydrolyze sodium hippurate.

Streptococcus agalactiae TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Streptococcus agalactiae TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM30650 (100 preps)	Cat. TM30610 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
S. agalactiae Primer & Probe Mix	280 μL	280 μL
S. agalactiae Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Description

Attogene’s Human IgG/IgM universal fluorescent lateral flow assay kit is a ready-to-use, universal test strip, which is based on the lateral flow technology that uses 655nm Emission Quantum Dot particles containing streptavidin to conveniently capture biotinylated antigens. The device is designed to easily develop qualitative or quantitative rapid test systems for detection of anti-human IgG and IgM antibody that react to the any antigen that can be biotinylated (i.e. viral antigen, autoimmune antigen) and is easily customizable providing every laboratory with the possibility to perform assay feasibility.

Formats (fluorescent broad range UV light excitation range of 300nm to 400nm, 610nm emission) Streptavidin conjugate pad):

Antibody tests are a method of choice to determine if a person has been exposed to a pathogen or not. They are also incredibly valuable in the detection of autoantibodies that can be found in human autoimmune disorders. In this test, a biotinylated antigen (User supplied) is mixed with a biotinylated rabbit IgG (bind to goat anti rabbit control line) and sample (human sera or plasma) is simply mixed into with the specially designed assay running buffer in a well of the supplied 96-well plate, mixed and is then added to the sample port of the cassette. Generally, the reaction is complete in 10-15 minutes. It is very important to note that the relative stoichiometry between the biotinylated antigen, biotinylated rabbit IgG added, and the streptavidin gold is critical for assay optimization. The appropriate concentration of biotinylated antigen to use with strips is dependent upon the purity and sequence and a standard curve can be used to determine the relative ratio (generally between 1ng-100ng per test). A positive control line (biotin-rabbit IgG) antibody will bind to the goat anti rabbit (GAR) line on the test to ensure the assay is running appropriately.