DBCO-PEG12-acid is an analog of DBCO-Acid with hydrophilic PEG linker and a DBCO group. The DBCO groups is commonly used for Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG12-acid is an analog of DBCO-Acid with hydrophilic PEG linker and a DBCO group. The DBCO groups is commonly used for Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
These kits provide a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing.
Plant/Fungi DNA Isolation Kit (Spin Column)
Complete 10 purifications in 45 minutes. This kit offers a maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.
Plant/Fungi DNA Isolation 96-Well Kit (High Throughput)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system. Complete 96 purifications in 50 minutes. This kit offers a maximum loading volume of 500 μL per well, and a maximum binding capacity of 50 μg per well.
Plant/Fungi DNA Isolation Kit (Magnetic Bead System)
The DNA is bound to the surface of the magnetic beads under optimized buffer conditions and released using a low salt buffer system. The Plant DNA Isolation Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations.
Plant/Fungi DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
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| Kit Specifications – Spin Column | |
| Column Binding Capacity | 50 μg |
| Maximum Column Loading Volume | 650 μL |
| Maximum Amount of Starting Material:Plant TissuesFungi (wet weight) | 100 mg 100 mg |
| Average Yields* 50 mg Tomato Leaves 50 mg Grape Leaves 50 mg Peach Leaves 50 mg Plum Leaves 50 mg Pine Needles Botrytis cinerea (50 mg wet weight) Fusarium sp. (50 mg wet weight) Aspergillus niger (50 mg wet weight) | 18 µg 10 µg 10 µg 10 µg 5 µg 1.5 µg 2 µg 4 µg |
| Time to Complete 10 Purifications | 45 minutes |
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature, except for the RNAse which should be stored at -20°C. This kit is stable for 1 year after the date of shipment.
Select Plants and Fungi that can be used with the Plant/Fungi DNA Purification Kits
| Plants | Plants (Cont’d) | Fungi |
| Tomato | Turnip | Aspergillus niger |
| Grape | Chinese Cabbage | Mucor racemosus |
| Peach | Radish | Cladosporium cladosporioides |
| Plum | Komatsuna | Fusarium oxysporum |
| Pine Needles | Apricot | Penicillium sp. |
| Raspberry | Sweet Potato | Botrytis cinerea (Botryotinia fuckeliana) |
| Strawberry | Hydrangea | Pichia sp. |
| Legumes | Fig | Rhizopus oryzae |
| Prosopis cineraria (Ghaf) | Turf | Alternaria tenuissima |
| Sorghum grass | Cherry | Fusarium sp. |
| Tobacco | Saintpaulia | |
| Arabidopsis | Lotus | |
| Lichen | Carrot | |
| Corn seed | Hansen | |
| Sunflower seed | Pistachio | |
| Olive seed | ||
| Soybean seed |
| Component | Cat. 26200 (50 preps) | Cat. 26250 (250 preps) | Cat. 26900 (192 preps) | Cat. 58200 (50 preps) | Cat. 62400 (192 preps) |
|---|---|---|---|---|---|
| Lysis Buffer L | 30 mL | 1 x 30 mL 2 x 60 mL | 2 x 60 mL | 60 mL | 2 x 60 mL |
| Binding Buffer I | 7 mL | 1 x 7 mL 1 x 25 mL | 25 mL | 7 mL | 25 mL |
| Solution WN | 18 mL | 1 x 18 mL 1 x 55 mL | 55 mL | 18 mL | 55 mL |
| Wash Solution A | 38 mL | 2 x 38 mL | 2 x 38 mL | – | – |
| Elution Buffer B | 15 mL | 30 mL | 30 mL | 8 mL | 30 mL |
| RNAse A | 1 vial (80 μL) | 5 vials (5 x 80 μL) | 1 vial | 1 vial | 1 vial |
| Magnetic Bead Suspension | – | – | – | 4 x 1.1 mL | 2 x 8.5 mL |
| Filter Columns | 50 | 250 | – | – | – |
| Spin Columns | 50 | 250 | – | – | – |
| Collection Tubes | 100 | 500 | – | – | – |
| 96-Well Plate | – | – | 2 | – | 2 |
| 96-Well Collection Plate | – | – | 2 | – | – |
| Adhesive Tape | – | – | 4 | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 250 | – | 50 | – |
| 96-Well Elution Plate | – | – | 2 | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 | 1 |
Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) offers a PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations. The kit consists of a specially designed primer mix compatible with the Illumina system, that is used in conjunction with the provided 2X Real-Time PCR Master Mix to amplify a library of unknown concentration. The unknown library is accurately quantified by using a standard curve constructed from the provided DNA Standard (range from 20 pM to 2 fM) on a Real-Time PCR System. The kit is specially optimized to quantify Small RNA-Seq libraries with DNA standards that have similar size to a Small RNA-Seq library. However, it could also be used with other types of NGS libraries.
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Storage Conditions
Upon receipt, store Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.
| Component | Cat. 61600 (100 reactions) |
|---|---|
| 2X Real-Time PCR Master Mix | 1 mL |
| NGS Library Quantification Primer Set Mix | 600 µL |
| Quantified NGS Library Standards (for Small-RNA Seq)* | 5 x 80 µL Standards |
| Product Insert | 1 |
* Quantified Library Standards are provided as 20 pM, 2 pM, 200 fM, 20 fM and 2 fM