bis-PEG4-endo-BCN is a homobifunctional click chemistry linker, PEG increase its aqueous solubility. The BCN group enable copper free click chemitry with azide-tagged molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
bis-PEG4-endo-BCN is a homobifunctional click chemistry linker, PEG increase its aqueous solubility. The BCN group enable copper free click chemitry with azide-tagged molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient.
The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen’s Plant/Fungi Total RNA Purification Kit is also available in a 96-well (High Throughput) format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.
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Click for expanded view
| Kit Specifications – Spin Column | |
| Maximum Column Binding Capacity | 50 μg |
| Maximum Column Loading Volume | 650 μL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material: Plant Tissues Plant Cells Fungi | 50 mg 1 x 106 cells 50 mg (wet weight) |
| Average Yield* 50 mg Tomato Leaves 50 mg Tobacco Leaves 50 mg Plum Leaves 50 mg Grape Leaves 50 mg Peach Leaves | 60 μg 60 μg 32 μg 35 μg 30 μg |
| Time to Complete 10 Purifications | 30 minutes |
* Yield will vary depending on the type of sample processed.
* Yield will vary depending on the type of sample processed.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Tobacco (Nicotiana tabacum)
Tomato (Lycopersicon esculentum)
Pepper (Capsicum annuum)
Potato (Solanum tuberosum)
Arabidopsis thaliana1
Peach (Prunus persica)
Apple (Malus sp.)
Pear (Pyrus sp.)
Grape vine (Vitis sp.)
Plum (Prunus sp.)
Palm (Arecaceae)
Pine needle (Pinaceae)
Strawberry
Raspberry
Blackberry
Herbs
Persimmon (Ebenaceae)
Potato tuber (Solanum)
Plum fruit
Citrus
Vanilla bean
Cotton (Gossypium)
Mangrove
Chrysanthemum
Grape berry skin
Kiwi leaves
Peach (fruits and flowers)
Soy bean (legume)
Eastern White Red Cedar
Corn leaves
Cucumber leaves
Aspergillus niger
Mucor racemosus
Cladosporium cladosporioides
Fusarium oxysporum
Penicillium sp.
Botrytis cinerea (Botryotinia fuckeliana)
Pichia sp.
Rhizopus oryzae
Alternaria tenuissima
| Component | Cat. 25800 (50 preps) | Cat. 31350 (100 preps) | Cat. 25850 (250 preps) | Cat. 31900 (192 preps) |
|---|---|---|---|---|
| Lysis Buffer C | 60 mL | 1 x 30 mL, 1 x 60 mL | 3 x 60 mL | 2 x 60 mL |
| Wash Solution A | 38 mL | 38 mL | 1 x 18 mL, 2 x 38 mL | 2 x 38 mL |
| Elution Solution A | 6 mL | 6 mL | 20 mL | 20 mL |
| Filter Columns | 50 | 100 | 250 | – |
| Spin Columns | 50 | 100 | 250 | – |
| 96-Well Plate | – | – | – | 2 |
| Adhesive Tape | – | – | – | 4 |
| Collection Tubes | 100 | 200 | 500 | – |
| 96-Well Collection Plate | – | – | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 100 | 250 | – |
| 96-Well Elution Plate | – | – | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 |
Features:
1.Brushless frequency motor,in great torque, free maintenance, no powder pollution, quick in speed up and down.
2.Imported compressors fluorine free,double cycle cooling,cold and hot alternating easily, free environment pollution and precise in temperature control.
3. Microprocessor control, digital display which indicates the speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.
4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
5. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.
6. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.
7. There are several kinds of rotors for your choice, adapters can be designed by experimental requirements.
8. 3 tiers protection steel cover and get the ideal centrifugation result.
TDL5M Technical Parameter：
| Max. Speed | 5000rpm |
| Max. RCF | 4730×g |
| Max. Capacity | 4×800ml( 4*450ml blood bags) |
| Time Range | 0~99h59min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 65 |
| Temperature | -20℃-40℃ |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | ±1℃ |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (W x D x Hmm) | 700×715×420mm |
| Net Weight(Kg) | 125KG |
| Certificates | CE,ISO & Calibration report are available |
Matched Rotors for TDL5M
| Order No. | Rotor | Max Speed (rpm) | Max Volume(ml) | Max. RCF（×g） |
| NO30671 | Swing Rotor | 4000 | 4×800ml(Round Cup) | 3450 |
| NO30696 | Swing Rotor | 4000 | 4×500ml( Round Cup) | 3380 |
| NO30679 | Swing Rotor | 4000 | 4×500ml(Square Cup) | 3310 |
| NO31494 | Microplate Rotor | 4000 | 4×4×96 Well | 2840 |
| NO31491 | 4000 | 2×4×96 Well | 2490 | |
| NO31376 | 4000 | 2×3×48 Well | 2300 | |
| NO30690 | Swing Rotor | 4000 | 4×30×7ml | 3270 |
| 4×30×5ml | 2890 | |||
| 4×18×10ml | 3140 | |||
| NO30607 | Angle Rotor | 5000 | 12×15ml | 3080 |
| NO30611 | Angle Rotor | 5000 | 6×50ml | 2850 |
| NO30640 | Angle Rotor | 5000 | 4×50ml | 2520 |
| NO30613 | Angle Rotor | 5000 | 4×100ml | 2630 |
| NO30639 | Angle Rotor | 5000 | 24×15ml | 3500 |
| NO30641 | Angle Rotor | 5000 | 12×50ml | 3860 |
| NO30614 | Angle Rotor | 5000 | 6×100ml | 3130 |
| NO30642 | Angle Rotor | 4000 | 12×100ml | 2970 |
Adapter for 800ml and 500ml bucket
| VolumeCup | 5ml | 7ml | 10ml | 15ml | 50ml |
| 800ml（Round Cup） | 23pcs/cup | 23 | 19 | 14 | 5 |
| 500ml（Round Cup） | 18pcs/cup | 18 | 14 | 9 | 3 |
| 500ml（Square Cup） | 20pcs/cup | 20 | 16 | 12 | 5 |