Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
50 & 150 reactions
E. coli O157:H7 is a rod-shaped, gram negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis. E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7). In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms. While most patients can recover from the infection, up to 15% of the patients may develop hemolytic uremic syndrome, a type of kidney failure that could be fatal. Infection of E. coli O157:H7 usually results from consumption of poorly prepared food including undercooked meat (particularly ground beef), untreated water or raw unpasteurized milk.
E.coli O157:H7 TaqMan PCR Kit, 100 reactions
E.coli O157:H7 TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
Figure 1 / 5
Click for expanded view
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM41350 (100 preps) | Cat. TM41310 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| E. coli O157:H7 Primer & Probe Mix | 280 μL | 280 μL |
| E. coli O157:H7 Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Name of Product
HAMA – Human Anti Mouse
Antibodies Detection Kit
Catalog Number
MQHM Z
Short Info
Lateral Flow Assay designed for the detection of Human Anti Mouse Antibodies (HAMA) in human serum.
This product is only available in the EU!
Method/Platform
Lateral Flow
Range/Assay Sensivity
97%
Test Principle
The HAMA Detection Kit is an immunoassay designed for qualitative determination of Human Anti Mouse Antibodies in human serum.
HAMA from patient’s sample bind first to mouse antibodies which are conjugated to gold nanoparticles.
In a second step the HAMA will be caught by mouse IgG antibodies, coated on the Test Line (T) of the nitrocellulose membrane.
In the presence of the HAMA-conjugat-complex a band becomes visible at the location of the Test Line (T).
The surplus of gold particles continues to migrate through the membrane and is captured at the Control Line (C) by specific antibodies.
Color’s intensitiy of the test line is directly proportional to the HAMA concentration in the sample.
Brief Instructions
Storage
2-8°C
Components
HAMA Test Unit, Chase Buffer, Evaluation Cards
5 tests
Lateral Flow Assay designed for the detection of Human Anti Mouse Antibodies (HAMA) in human serum.