The Residual DNA Sample Preparation Kit uses chemical lysis and magnetic beads to extract DNA from diverse sample types, including samples that contain high protein and low DNA concentration. The kit extracts residual genomic DNA from products that are produced in cell lines such as CHO, E. coli, E1A, HEK293, Vero, NS0 and Baculovirus. For quantification of residual DNA, we recommend using the resDNASEQ Residual DNA Quantitation kit as described in the resDNASEQ Residual DNA Quantitation kit User Guide. To ensure accurate quantitative results, each sample in triplicate and perform a single PCR reaction for each extraction.
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Only three steps will be need for Sample Preparation，and the total time of Sample preparation is about 30 minites.
The good Recovery rate of Sample Preparation.
All components of the Sample Preparation Kit can be stored at room temperature.
Note: Price not include shipment & duty, contact us to get full quote.
The Residual DNA Sample Preparation Kit uses chemical lysis and magnetic beads to extract DNA from diverse sample types, including samples that contain high protein and low DNA concentration. The kit extracts residual genomic DNA from products that are produced in cell lines. The operation is easier and the higher nucleic acid yield is guaranteed at the same time.
K-AGLUA
50 assays (manual) / 200 assays (microplate)
| Content: | 50 assays (manual) / 200 assays (microplate) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | α-Glucuronidase |
| Assay Format: | Spectrophotometer, Microplate |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 17.4 to 174 mU/mL of α-D-glucuronidase per assay |
| Limit of Detection: | 17 mU/mL |
| Reaction Time (min): | ~ 25 min |
| Application examples: | Enzyme preparations and other materials. |
| Method recognition: | Novel method |
This product has been discontinued (read more).
The α-D-Glucuronidase test kit is a simple, reliable and accurate method for the measurement and analysis of α-D-glucuronidase in various enzyme preparations. The kit contains a pure aldotriouronic acid substrate and an α-D-glucuronidase (GH67) control enzyme.
New, improved substrate.
This kit contains highly purified, borohydride reduced aldotriouronic acid with a terminal α-D-glucuronic acid substitution, which is an excellent substrate for α-D-glucuronidases from GH67. This substrate can be also used for the measurement of α-D-glucuronidases from GH115, however the rate of hydrolysis compared to GH67 is reduced. Previously this kit contained a mixture of borohydride reduced aldouronic acids (tri:tetra:penta).
View more test kits for enzyme activity measurement.
Advantages
The α-D-Glucuronidase test kit is a simple, reliable and accurate method for the measurement and analysis of α-D-glucuronidase in various enzyme preparations. The kit contains a pure aldotriouronic acid substrate and an α-D-glucuronidase (GH67) control enzyme.
Product Description
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | DNA Isothermal Amplification Kit NFO |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.