This kit is used for extracting total pathongen nucleic acid from low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate, culture, etc. The purified DNA/RNA is ready for downstream clinical in vitro detection such as Real Time PCR, biochip analysis, NGS and other related detection.
Specifications
| Features | Specifications |
| Main Functions | Extract pathogen DNA/RNA from whole blood, body fluid, serum, plasma, sputum, immersion solution, tissue homogenate supernatant, etc. |
| Applications | RT-PCR，PCR，NGS |
| Products | Pathogen DNA and RNA |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Whole blood, plasma, serum, immersion solution, tissue homogenate supernatant |
| Sample amount | 200μl |
| Elution volume | ≥30μl |
| Time per run | 30 mins |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA/RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA is finally eluted with low-salt buffer (10 MmTris, pH 8.0).
Advantages
Kit Contents
| Contents | IVD4179 |
| Purification Times | 50 Preps |
| HiPure Viral Mini Column | 50 |
| 2ml Collection Tubes | 100 |
| 2ml Bead Tubes | 50 |
| Protease K | 50 mg |
| Protease Dissolve Buffer | 5 ml |
| DNase I (Powder) | 10 mg |
| DNase Buffer | 6 ml |
| Buffer CLB | 100 ml |
| Buffer SDS | 5 ml |
| Reagent DX | 1.5 ml |
| Buffer ACL | 30 ml |
| Buffer VHB* | 22 ml |
| Buffer RW2* | 20 ml |
| Buffer AVE | 15 ml |
Storage and Stability
Proteinase K and DNase I (Powder) should be stored at 2–8°C upon arrival. However, short-term storage (DNase I up to 1 week, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affecttheir performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
This kit is used for extracting total pathongen nucleic acid from low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate, culture, etc. The purified DNA/RNA is ready for downstream clinical in vitro detection such as Real Time PCR, biochip analysis, NGS and other related detection.
The NGS Library Quantification Standards with PCR Primers (for illumina platform) were developed for quantification of the NGS library concentration for illumina sequencing platform. It is comprised of Library Standards (six 10-fold dilutions) and a primer mix.
Quantification of the NGS library of the amplifiable molecules is critical for the quality of the sequencing data. Adequate library concentration will maximize sequencing capacity. Poor library concentration results in either low or high cluster density on the flow cell, which can lead to low sequencing capacity.
It is not accurate to measure the concentration of NGS library with standard DNA quantification methods such as spectrophotometer or fluorometer. QPCR is the best way for library quantification with high consistency and reproducibility of library quantitation.
BioDynami Library Quantification Standards with PCR Primers (for illumina platform): Amplification curve of 6 standards.
Our reagent is a highly sensitive, Real time PCR-based quantification that specifically designed for NGS libraries using illumina sequencing platform. The amplification uses illumina adaptor sequences as primers, and only the fully adaptor-ligated libraries will be amplified. Therefore the reagent provides an accurate estimation of the library concentration based on true sequenceable illumina libraries. In addition, this kit can also be used for confirmation of ligation reaction after completion of library preparation.
Our library quantification standards is compatible with commercial SYBR Green based QPCR reagents. This makes it more flexible for scientists who want to use their real time PCR reagent. Quantification of library concentration is achieved by comparison with a standard curve generated from the Library Standards.
The NGS Library Quantification Standards with PCR Primers (for illumina platform) were developed for quantification of the NGS library concentration for illumina sequencing platform. It is comprised of Library Standards (six 10-fold dilutions) and a primer mix.
Opentrons OT-2 Tips, 300µL. Optimized for volumes 5µL to 300µL Clear Tips, Polypropylene. These tips are DNAse/RNAse, pyrogen and protease free – they are sterilized from ebeam irradiation. Not autoclavable.
Compatible with: Opentrons 8-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN1 P50 Electronic Pipettes, Opentrons Single-Channel GEN1 P300 Electronic Pipettes, Opentrons 8-Channel GEN1 P50 Electronic Pipettes, Opentrons 8-Channel GEN1 P300 Electronic Pipettes.
Opentrons OT-2 Tips, 300µL. Optimized for volumes 5µL to 300µL Clear Tips, Polypropylene. These tips are DNAse/RNAse, pyrogen and protease free – they are sterilized from ebeam irradiation. Not autoclavable.
Compatible with: Opentrons 8-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN1 P50 Electronic Pipettes, Opentrons Single-Channel GEN1 P300 Electronic Pipettes, Opentrons 8-Channel GEN1 P50 Electronic Pipettes, Opentrons 8-Channel GEN1 P300 Electronic Pipettes.