The Hepatitis B virus (HBV) is mainly transmitted via blood or blood products. In addition, sexual, oral and perinatal infections are also possible. Early symptoms of the infection include appetite loss, vomiting and abdominal symptoms, with approximately 10-20% of those patients developing fever as well as rheumatoid joint and muscle pain. Jaundice, which may be accompanied by itching, will then develop within 2-14 days. Fulminant hepatitis then occurs in about 1% of all infected patients, which in severe cases may be fatal. Of those individuals infected by HBV, 5-10% will develop chronic liver inflammation which may progress to cirrhosis of the liver or, in the worst case, primary liver cell carcinoma. The HBV virus is a hepadnavirus that has a circular genome composed of partially double-stranded DNA.
HBV TaqMan PCR Kit, 100 reactions
HBV TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM29250 (100 rxns) | Cat. TM29210 (100 rxns) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| HBV Primer & Probe Mix | 280 μL | 280 μL |
| HBV Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
MagPure Plant DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and tissues.The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo.Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridization, and transgenosis detection.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 50-100 mg plant, fungal tissue |
| Applications | PCR, transgene detection, fluorescence quantitative PCR, southern blot, SNP site analysis, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Conventional economic plant samples |
| Sample amount | Fresh / frozen plant samples:≤ 100mgDried plant / seed samples:≤ 20mg |
| Elution volume | ≥50μl |
| Time per run | ≤60 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol toremove salts, and finally DNA was eluted by Elution Buffer.
Kit Contents
| Contents | D635101 | D635102 | D635103 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagPure Particles | 1.7 ml | 4 ml | 18 ml |
| RNase Solution | 0.6 ml | 1.2 ml | 6 ml |
| Buffer SPL | 30 ml | 60 ml | 300 ml |
| Buffer PS | 10 ml | 20 ml | 100 ml |
| Buffer GW1* | 26 ml | 53 ml | 220 ml |
| Elution Buffer | 10 ml | 30 ml | 120 ml |
Storage and Stability
RNase Solution and MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
MagPure Plant DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and tissues.The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo.Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridization, and transgenosis detection.
For selective isolation and culture of Staphylococcus aureus.
Peptone and beef extract powder provides carbon, nitrogen, vitamins and minerals; D- mannitol to fermentable sugars; higher levels of sodium chloride to provide a higher osmotic pressure, suppress most non-staphylococcal microorganisms ; phenolsulfonphthalein as pH indicator; agar is medium coagulant. Typical pathogenic staphylococci (coagulase positive) D- mannitol produce acid fermentation and produce yellow colonies with a yellow halo, typically non-pathogenic Staphylococcus unfermented D- mannitol to form red colonies.
Formulation(per liter):
Pancreatic digest of casein 5.0g
Pancreatic digest of animal tissue 5.0g
Beef Extract 1.0g
Sodium Chloride 75.0g
Mannitol 10.0g
Phenol Red 0.025g
Agar 15.0g
Final PH 7.4±0.2
How to use：
1.Suspend 111g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
Quality control：
| Item | The name and number of strain | Growth | Colony Color |
| 1 | Staphylococcus aureus CMCC (B) 26003 | Good | Golden yellow |
| 2 | Staphylococcus epidermidis CMCC (B) 26069 | Good | Red |
| 3 | Escherichia coli CMCC (B) 44102 | Inhibition | — |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle
250g