West Nile Virus (WNV) belongs to the RNA virus family of Flaviviridae. It is known to spread primarily through arthropod vectors such as mosquitoes. The virus infects mainly birds but is also reported to infect humans as well as pets such as cats and dogs. In humans, the majority of the infections caused by WNV are asymptomatic. However, some individuals (the majority of the confirmed cases) could enter a second, febrile stage with flu-like symptoms – commonly known as West Nile Fever. In a more serious stage, the disease becomes neuroinvasive, causing meningitis or encephalitis. Such severe conditions could lead to mortality. As the symptoms of WNV are very similar to other common diseases but can be fatal at a severe stage, it is important to distinguish the virus early in the diagnosis, particularly at the molecular level.
WNV TaqMan RT-PCR Kit, 100 reactions
WNV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM44250 (100 preps) | Cat. TM44210 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| WNV Primer & Probe Mix | 280 μL | 280 μL |
| WNV Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Propargyl-PEG5-amine is a propargyl linker that is commonly used as a Click Chemistry reagent for copper-catalyzed reactions with azides. The amine moiety reacts with carboxylic acids, activated NHS esters and other carbonyl compounds. The PEG spacer helps improve the water solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG5-amine is a propargyl linker that is commonly used as a Click Chemistry reagent for copper-catalyzed reactions with azides. The amine moiety reacts with carboxylic acids, activated NHS esters and other carbonyl compounds. The PEG spacer helps improve the water solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
K-ASPTM
50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)
| Content: | 50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Aspartame |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Decrease |
| Linear Range: | 10 to 150 µg of aspartame per assay |
| Limit of Detection: | 0.57 mg/L |
| Reaction Time (min): | ~ 5 min |
| Application examples: | Soft drinks, artificial sweeteners, candies, mints, chewing gum, dietetic products, jam, chocolate and other materials. |
| Method recognition: | Novel method |
This product has been discontinued (read more).
The Aspartame test kit is a simple and reliable method for the specific measurement and analysis of Aspartame in beverages and foodstuffs.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
We have a wide range of other assay kit products.
Advantages
The Aspartame test kit is a simple and reliable method for the specific measurement and analysis of Aspartame in beverages and foodstuffs.