Solid Phase Reversible Immobilization magnetic beads consist of paramagnetic particles coated with carboxyl groups that reversibly bind DNA. They are used for DNA purification because they are fast, simple and efficient. Our Magnetic Beads (PCR Purification) combines BioDynami’s proprietary chemistries with reversible DNA-binding properties of magnetic beads. The beads are developed for effective PCR fragment purification by removing primers and unwanted components such as salts, dNTPs, enzymes, and others.
Our Magnetic beads (PCR Purification) are optimized to selectively bind PCR fragments of 80 bp and larger and remove primers that are 30 nt and shorter. Purified PCR fragments are suitable for any downstream applications. The beads can be used for PCR cloning, PCR cleanup, or even PCR fragment concentration.
Features:
Applications:
Solid Phase Reversible Immobilization magnetic beads consist of paramagnetic particles coated with carboxyl groups that reversibly bind DNA. They are used for DNA purification because they are fast, simple and efficient. Our Magnetic Beads (PCR Purification) combines BioDynami’s proprietary chemistries with reversible DNA-binding properties of magnetic beads. The beads are developed for effective PCR fragment purification by removing primers and unwanted components such as salts, dNTPs, enzymes, and others.
Propargyl-PEG13-acid consists of an alkyne group and a carboxylic acid. The carboxylic acid reacts with amine groups to form amide bonds in the presence of EDC or HATU as an activator. The alkyne group is commonly used in copper catalyzed azide-alkyne Click Chemistry reactions. The hydrophilic PEG units enhance the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG13-acid consists of an alkyne group and a carboxylic acid. The carboxylic acid reacts with amine groups to form amide bonds in the presence of EDC or HATU as an activator. The alkyne group is commonly used in copper catalyzed azide-alkyne Click Chemistry reactions. The hydrophilic PEG units enhance the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This product provide fast and easy methods for purification of total DNA from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells. There is no need to use toxic phenol chloroform extraction or time-consuming alcohol precipitation. The extraction process finish in 40 minutes. Purified DNA includes genomic DNA, mitochondrial DNA, viral DNA (e.g. HBV), or DNA from other parasitic microorganisms. The obtained DNA can be directly used in PCR, viral DNA detection and other experiments.
This kit can use on manual protocol or 16/32/48 channel automated extraction system. No need to pause for the addition of binding solution (not applicable to 96/192 instruments)
| Contents | D631001C | D631002C | D631003C |
| Purification Times | 48 | 96 | 480 |
| MagPure Particles | 1.7 ml | 3.5 ml | 16 ml |
| Proteinase K | 24 mg | 50 mg | 220 mg |
| Protease Dissolve Buffer | 1.8 ml | 3 ml | 15 ml |
| Buffer MLA | 30 ml | 70 ml | 300 ml |
| Buffer DW1 | 30 ml | 70 ml | 300 ml |
| Buffer EW | 60 ml | 120 ml | 2 x 300 ml |
| Buffer MWX1 | 30 ml | 70 ml | 300 ml |
| Elution Buffer | 10 ml | 20 ml | 60 ml |
Prefilled Kit Contents
| Product | Contents and volume | D6310-TL-06C | D6310-S-48 |
| Proteinase K | 50 mg | 24 mg | |
| Protease Dissolve Buffer | 3 ml | 1.8 ml | |
| TL-Tip | 12 pcs | 24 pcs | |
| Tip bottom plate/ Tip base reagent strip | Row 1/7：600μl Buffer MLA | 6 plates | 48 strips |
| Row 2/8：600μl Buffer MWX1 | |||
| Row 3/9：600μl Buffer DW1 | |||
| Row 4/10：30μl MagPure Particles 600μl Buffer EW | |||
| Row 5/11：600μl Buffer EW | |||
| Row 6/12：100μl Elution Buffer |
Storage and Stability
Proteinase K Powder should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these condition.
This product provide fast and easy methods for purification of total DNA from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells. There is no need to use toxic phenol chloroform extraction or time-consuming alcohol precipitation. The extraction process finish in 40 minutes. Purified DNA includes genomic DNA, mitochondrial DNA, viral DNA (e.g. HBV), or DNA from other parasitic microorganisms. The obtained DNA can be directly used in PCR, viral DNA detection and other experiments.