Description
The DM5100 ExcelBand™ Super Range DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder DM5100 is composed of 26 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1.2k, 1k, 900, 800, 700, 600, 500, 450, 400, 350, 300, 250, 200, 150, 100 and 50 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains four enhanced bands (3k, 1.2k, 500 and 200 bp) for easier reference. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are added for real time monitoring.
Features
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
50 ~ 25,000 bp
Concentration
88.7 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Room temperature for 6 months
4°C for 12 months
-20°C for 36 months
This kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured animal cells, small tissue samples, bacterial cells, plants, and blood.
Two columns are provided with this kit. The first column captures large RNA, while the small RNA are captured on a second column and are eluted concentrated in 25 µL of nuclease-free water.
The small RNA can be used in various downstream applications relating to miRNA profiling, gene regulation, and functional analysis. The eluted RNA is ready for RT-qPCR, microarrays, and NGS applications.
Background
These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long and regulate gene expression by binding to mRNA molecules and affecting their stability or translation.
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| Kit Specifications | |
| Maximum Column Binding Capacity | Up to 50 μg RNA |
| Maximum Column Loading Volume | 650 μL |
| Minimum Elution Volume | 20 μL |
| Size of RNA Purified | All sizes, including small RNA < 200 nt |
| Time to Complete 10 Purifications | 25 minutes |
| Maximum Amount of Starting Material: Animal Cells Animal Tissues Bacteria Plant Tissues Blood | 3 x 106 cells 5-25 mg 1 x 109 cells 50 mg 100 μL |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 21300 (25 preps) |
|---|---|
| Buffer RL | 40 mL |
| Wash Solution A | 38 mL |
| Elution Solution A | 6 mL |
| Large RNA Removal Column | 25 |
| microRNA Enrichment Column | 25 |
| Collection Tube | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.
Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.
Our SNPsig® technology provides an alternative to sequencing as well as S gene target failure (SGTF) that enables scientists to analyse and monitor these specific genomic mutations. Our kits can provide a pivotal role in screening for SARS-CoV-2 variants for the purpose of genomic surveillance and studies.
For the detection of the SARS-CoV-2 variants with the N501Y mutation (UK, South Africa and Brazil)
Rapid detection of specific detection profiles
High priming efficiency
Sensitive to < 100 copies of target
Positive copy number standard curve for quantification
Accurate controls to confirm findings
96 reactions, includes master mix