Description
The DM3200 ExcelBand™ 1 KB Plus (0.1-10 kb) DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder DM3200 is composed of 19 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200, and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains three enhanced bands (3 kb, 1.5 kb and 500 bp) for easier reference. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring.
Features
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
100 ~ 10,000 bp
Concentration
87 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Room temperature for 6 months
4°C for 12 months
-20°C for 36 months
The DM3200 ExcelBand™ 1 KB Plus (0.1-10 kb) DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder DM3200 is composed of 19 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200, and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains three enhanced bands (3 kb, 1.5 kb and 500 bp) for easier reference. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring.
Product Details
Application:
DNA Nucleic Acid Amplification
Brand:
Amp-future Bio
Components:
Polymerase, Buffer
Name:
DNA Isothermal Rapid Amplification Kit(Basic)
Reaction Time:
5-20mins
Sensitivity:
High
Shelf Life:
14 Months
Size:
50 Reactions
Storage:
-20°C
Type:
Basic
High Light:
,
,
Payment & Shipping Terms
Minimum Order Quantity
48T
Price
USD$3.8/T
Packaging Details
16T/bags,48T/Box
Delivery Time
14days
Payment Terms
T/T, , MoneyGram
Supply Ability
100000T/Months
Product Description
| Reagent component ( WLB8201KIT ,16T/bags,48T/Box ) | |||
| Component | Specification | Quantity | Function |
| A buffer | 1.6ml | 1 Tube | Buffer system mainly for stabilizing protein/enzyme and performance |
| B buffer | 0.15ml | 1 Tube | Mainly activated systems such as magnesium ions |
| Positive control template | 0.1ml | 1 Tube | Mainly the positive plasmid template is used to test the effectiveness of the kit |
| Positive control primer mix | 0.06ml | 1 Tube | Mainly the primer combination of the positive control template |
| Reagent Guide Manua | 16T/bags,48T/Box | 3 bags | Reagent technology of protein/enzyme system: freeze-dried powder, freeze-dried microspheres |
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39ºC~42ºC), with the help of auxiliary proteins and single-strand binding proteins,the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins;along with the dissociation of the recombinase from the complex,the polymerase also binds to the 3′ end of the primer and begins chain extension.It is suitable for laboratory-level DNA amplification and DNA amplification for other detection purposes.
