Propargyl-PEG4-thiol is a crosslinker containing a propargyl group and thiol group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reactions. The thiol group reacts with maleimide, OPSS, vinylsulfone and transition metal surfaces including gold, silver, etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG4-thiol is a crosslinker containing a propargyl group and thiol group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reactions. The thiol group reacts with maleimide, OPSS, vinylsulfone and transition metal surfaces including gold, silver, etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Description
The DM2160 FluoroBand™ 100 bp Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM2160 is composed of 11 individual DNA fragments: 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Features
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
100 ~ 1,500 bp
Concentration
52.2 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Protected from light
Room temperature for 6 months
4°C for 12 months
-20°C for 24 months
The DM2160 FluoroBand™ 100 bp Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM2160 is composed of 11 individual DNA fragments: 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Herpes Simplex Virus 1 (HSV-1) is a member of the herpes virus family, Herpesviridae. HSV-1 has a relatively large double-stranded DNA genome. HSVs are primarily transmitted by sexual intercourse, direct contact with lesions or perinatally. Most HSV positive cases are characterised by lesions on the skins and mucous membranes of the mouth and genitals. HSV infection can be either primary or a recurrence of a previous infection. More than 90% of the primary HSV infections are asymptomatic. Primary infection with HSV-1 can lead to gingivostomatitis, eczema herpeticum, keratoconjunctivitis and encephalitis. The primary symptoms of a secondary infection are skin lesions in the nose, mouth and genital regions. The infection is contagious, mainly during an epidemic.
HSV-1 TaqMan PCR Kit, 100 reactions
HSV-1 TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM32650 (100 preps) | Cat. TM32610 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| HSV-1 Primer & Probe Mix | 280 μL | 280 μL |
| HSV-1 Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |