750ml Tube (Low Speed)

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Eleven discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
• Higher intensity reference band at 500 bp

The Norgen LowRanger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our LowRanger contains eleven discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments with a higher intensity reference band at 500 bp. This Ladder is ideal for fast running times and accurate visual determination.

Contents:

1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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LowRanger 100bp DNA Ladder (Cat# 11500) – 100 loads

Ladder Properties:
• Eleven discrete bands, ranging from 100 bp to 2000 bp
• Higher intensity band at 500 bp for easy reference.

Fragment	Size (bp)	Mass (ng)
1	2000	110
2	1500	83
3	1000	55
4	800	44
5	700	39
6	600	33
7	500	55
8	400	22
9	300	17
10	200	22
11	100	22

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 
Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Isotherm3G is a mesophilic DNA polymerase, perfectly suited for Isothermal amplifications. Isotherm3G has been mutated to have improved reverse transcription activity. It synthesizes DNA from both DNA and RNA templates with a high strand displacement activity allowing simplified one-enzyme RT-LAMP reactions.

Isotherm3G exhibits 5’ to 3’ polymerase activity but lacks any exonuclease activity. The product components have been optimized and are perfectly suited for loop-mediated isothermal amplification (LAMP) with optimal reaction temperature at 65°C.

Isotherm3G DNA Polymerase utilizes an aptamer-based warm-start feature to prevent false amplification at low temperatures. For more information, please contact us. 

The product is available also in bulk quantities with custom fillings and can be produced customized for example in a lyo-ready formulation. 

Isotherm3G is a mesophilic DNA polymerase, perfectly suited for Isothermal amplifications. Isotherm3G has been mutated to have improved reverse transcription activity. It synthesizes DNA from both DNA and RNA templates with a high strand displacement activity allowing simplified one-enzyme RT-LAMP reactions.

Overview

• Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
• Isolate total RNA, including microRNA, without phenol
• Rapid and convenient spin-column format and 96-well plate for high throughput applications
• Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.

Leukocyte RNA Purification Kit (Spin Column)

This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. Allowable blood input ranges from 10 μL to 2 mL or 3 x 10⁶ Leukocytes

Leukocyte RNA Purification Plus Kit (Plus)

Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. Complete 10 purifications in as little as 40 minutes.

Leukocyte RNA Purification 96-Well Kit (High Throughput)

Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen’s kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 10⁶ Leukocytes.

Details

Supporting Data

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Kit Specifications
Maximum Column Binding Capacity	10 µg
Maximum Column Loading Volume	650 μL
Size of RNA Purified	All sizes, including small  RNA (<200 nt)
Maximum Blood Input Volume	3 mL
Time to Complete 10 Purifications	40 minutes

Storage Conditions and Product Stability 

All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.

Component	Cat. 21200 (50 preps)	Cat. 21250 (50 preps)	Cat. 37800 (2 x 96 preps)
RBC Lysis Buffer	2 x 100 mL	2 x 1 L	2 x 90 mL
Buffer RL	30 mL	30 mL	–
Binding Solution	–	–	2 x 40 mL
Wash Solution A	38 mL	38 mL	–
Wash Solution	–	–	2 x 30 mL
Elution Solution A	6 mL	6 mL	–
Elution Solution	–	–	2 x 20 mL
Enzyme Incubation Buffer	–	6 mL	1
DNase I	–	1 Vial	–
Mini Spin Columns	50	–	–
Lysate Homogenization Column	–	50	–
Single Cell RNA Column	–	50	–
RBC Lysis 96-Well Plate	–	–	2
96-Well Filter Plate	–	–	2
Adhesive Tape	–	–	4
Collection Tubes	50	100	–
96-Well Collection Plate	–	–	2
Elution Tubes (1.7 mL)	50	50	–
96-Well Elution Plate	–	–	2
Product Insert	1	1	1