Norgen’s Low Abundance DNA Quantification Kit offers a PCR-based detection procedure to quantify DNA of a wide spectrum of concentrations, including the lower ng per µL, pg per µL and sub-pg per µL range. The kit consists of a specially designed primer mix, that is used in conjunction with the provided 2x PCR Master Mix, to amplify human DNA from different types of inputs (such as various liquid biopsies). The kit is compatible with any Real-Time PCR system with the addition of fluorescent nucleic acid stains such as SYBR Green. The unknown DNA is accurately quantified by using a standard curve constructed from the provided DNA Standard.
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Storage Conditions
Upon receipt, store Norgen’s Low Abundance DNA Quantification Kit at -20°C or lower. Avoid multiple freeze-thaw cycle. If needed, prepare smaller working aliquots and store at -20°C or lower.
| Component | Cat. 57200 (48 reactions) |
|---|---|
| 2X PCR Master Mix | 1 mL |
| DNA Quantification Primer Set Mix | 200 µL |
| Quantified DNA Standard | 5 standards, each 100 µL |
| Nuclease-Free Water | 1.25 mL |
| Product Insert | 1 |
K-LACGAR
SKU: 700004307
115 assays per kit
| Content: | 115 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | D-Galactose, Lactose |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 4 to 80 µg of D-galactose (or 8 to 160 µg of lactose) per assay |
| Limit of Detection: | 2.96 mg/L |
| Reaction Time (min): | ~ 15 min |
| Application examples: | Milk, dairy products (e.g. cream, milk / whey powder, cheese, condensed milk and yogurt), foods containing milk (e.g. dietetic foods, bakery products, baby food, chocolate, sweets and ice-cream), food additives, feed, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Methods based on this principle have been accepted by AOAC Method 2006.06, NBN, DIN, GOST and IDF |
The Lactose/Galactose (Rapid) test kit is used for the rapid test of lactose, D-galactose and L-arabinose in food and plant products. Galactose dehydrogenase can be used the measurement and analysis of both D-galactose and L-arabinose. Suitable for the analysis of lactose in “low-lactose” or “lactose-free” samples which contain high levels of monosaccharides. The reagents provided in this kit are also suitable for use with AOAC method 2006.06 – Lactose in milk.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
View our full range of mono/disaccharide test kits.
Advantages
The Lactose/Galactose (Rapid) test kit is used for the rapid test of lactose, D-galactose and L-arabinose in food and plant products. Galactose dehydrogenase can be used the measurement and analysis of both D-galactose and L-arabinose. Suitable for the analysis of lactose in “low-lactose” or “lactose-free” samples which contain high levels of monosaccharides. The reagents provided in this kit are also suitable for use with AOAC method 2006.06 – Lactose in milk.
