81 Tube Lab Freezer Box with attached Lid (pack of 10)
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【Material】The vial storage box is made of durable cardboard with waterproof coating and cardboard dividers；chemically resistant to alcohols and mild organic
【Temperature Range】These freezer boxes are stable from -196°C to 121°C
【Applications】The freezer box is suitable for iquid nitrogen freezing (vapor phase)
【Capacity】Size: Height of 2 inch, Holds 0.5ml, 1.5ml, 2.0ml tubes
【Excellent Cusomer Service】We are dedicated in providing the best products and services to customers. If you have any problem, please feel free to contact us. We will help you solve the problem as soon as possible.
【Material】The vial storage box is made of durable cardboard with waterproof coating and cardboard dividers；chemically resistant to alcohols and mild organic
【Temperature Range】These freezer boxes are stable from -196°C to 121°C
【Applications】The freezer box is suitable for iquid nitrogen freezing (vapor phase)
【Capacity】Size: Height of 2 inch, Holds 0.5ml, 1.5ml, 2.0ml tubes
【Excellent Cusomer Service】We are dedicated in providing the best products and services to customers. If you have any problem, please feel free to contact us. We will help you solve the problem as soon as possible.
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Bioprocessing with Salt Active Nucleases – Physiological Conditions
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Medium-Salt Active Nuclease High Quality (M-SAN HQ) is a Bioprocessing Grade nuclease developed for removal of both single and double-stranded DNA and RNA at the physiological salt conditions most often used in bioprocessing and biomanufacturing workflows. M-SAN HQ allows you to directly replace Benzonase without changing your workflow.
This novel, nonspecific endonuclease is active over a broad pH range and displays optimum activity at salt concentrations between 125 – 250 mM. Due to its excellent performance at physiological conditions, M-SAN HQ can be used directly in the cell medium or the harvested supernatant without buffer adjustments. This makes M-SAN HQ ideal for the manufacturing of fragile vectors such as lentiviruses and retroviruses.
M-SAN HQ can be directly used in medium without buffer adjustments The high activity of M-SAN HQ at standard cell medium conditions leads to improved DNA clearance compared to other commonly used nucleases. In the data (see Figure 6), an over 2-fold reduction in residual DNA was achieved.
Key Benefits
Compatibility: Ideal for working with both fragile and robust viral vectors and proteins in a variety of cell media
Optimum activity: Optimization for cell media salinity allows both shorter DNA fragments and reduced incubation times.
Cost-Effectiveness: Reduced need for additional reagents and steps can lower production costs
Quality: Maintains the integrity of sensitive or labile biological molecules, ensuring a higher quality end product
Flexibility: Can be used directly in a variety of media without the need for customization
Key Features
High purity (≥ 99%)
No protease detected
Endotoxin-tested
Animal origin-free production
Supplied with extended product documentation
Adapted to use in medium without salinity adjustments
Optimal activity at physiological salinity and pH makes it ideal for DNA removal from mammalian cell media. (see Figures)
The high activity of M-SAN HQ at the physiological salinity and pH found in standard cell medium conditions leads to improved DNA clearance compared to commonly used nucleases.
Simple to Optimize in Cell Media
M-SAN HQ is uniquely formulated to excel in physiological pH conditions, offering high performance at the commonly used cell media pH of 7.4.
While other nucleases often require more alkaline environments, M-SAN HQ stands out for its adaptability and effectiveness in cell media. It’s the nuclease that truly aligns with your bioprocessing needs. (See Figure section)
M-SAN HQ ELISA Kit
The M-SAN HQ ELISA Kit confirms the removal of M-SAN High Quality in bioprocessing and biomanufacturing applications with high accuracy:
Medium-Salt Active Nuclease High Quality (M-SAN HQ) is a Bioprocessing Grade nuclease developed for removal of both single and double-stranded DNA and RNA at the physiological salt conditions most often used in bioprocessing and biomanufacturing workflows. M-SAN HQ allows you to directly replace Benzonase without changing your workflow.
IsoPol® BST+ is a heat-tolerant BST polymerase (large fragment) with enhanced strand-displacement activity. IsoPol® BST+ is active from 25 to 65°C. It is lacking 5’-3’- and 3’-5’-exonuclease activity.
Key features
IsoPol® BST+ lacks 5’-3’- and 3’-5’-exonuclease activity.
Inactivation – IsoPol® BST+ is completely inactivated after one minute incubation at 95°C
Enhanced strand displacement and processivity.
Increased salt tolerance.
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Key features
High concentration
High purity
Lyophilisation and automation friendly
Figures
Properties
Document
These DNA polymerases are ideal for Isothermal Amplification
IsoPol® BST+ is a heat-tolerant BST polymerase (large fragment) with enhanced strand-displacement activity. IsoPol® BST+ is active from 25 to 65°C. It is lacking 5’-3’- and 3’-5’-exonuclease activity.
