Specification
| 7-50x continuous zoom stereo microscope | XTL-205A | XTL-205B | XTL-204A | XTL-2048 | XTL-203A | XTL-203B | |
| Zoom Body | Binocular head,45°decline,360°rotate | ● | ● | ● | |||
| Trinocular head,45°decline,360*rotate | ● | ● | ● | ||||
| Eyepiece | WF10X22mm | ● | ● | ● | ● | ● | ● |
| Objective | zoom objective 0.7X—5X | ● | ● | ● | ● | ● | ● |
| Zoom Rate | 0.1:07.1 | ● | ● | ● | ● | ● | ● |
| Total Amplific- ation Rate | the standard configuration is 7X-50X | ● | ● | ● | ● | ● | ● |
| Auxiliary Lens | 0.5X,0.7X,1X,1.5X,2X For Option | O | O | O | O | O | O |
| WorkingDistance | 100mm | ● | ● | ● | ● | ● | ● |
| Diopter | 55mm-75mm | ● | ● | ● | ● | ● | ● |
| Focus arm | 76mm Diameter,32mm pole size | ● | ● | ● | ● | ● | ● |
| Plate | Black/White plate,Glass plate for option | ● | ● | ● | ● | ● | ● |
| Optional | Stand | Q | Q | ||||
| Stand | BI3 base.column sunnort unner and ower light source 3 WLED | ● | ● | ||||
| BL3-A base vertical arm bracket upper and lower light source 3 WLED | ● | ● | |||||
| B3 base column support has no light source | ● | ● | |||||
| External light source | LED illumination | Q | Q | Q | O |  | |
| Optiona | Photography,camera accessories CCD Coupler | o | o | o | o | o | o |
| Optional | Display attachment Monitor | | |||||
The Small RNA Library Prep Kit for Illumina consists of all the reagents and components required to generate small RNA libraries to be used for next-generation sequencing on an Illumina platform. All molecular reagents including adaptors, primers, enzyme mixes and buffers are provided. A purification module is also provided for rapid purification of nucleic acid products generated at various steps of the workflow. The purification module utilizes Norgen’s patent resin technology which enhances recovery of desired library intermediates or final products. The library prep workflow could be used for different forms of input including purified total RNA or enriched small RNA, as well as RNA from low content inputs such as plasma, serum and urine.
Workflow
Figure 1 / 4
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| Kit Specifications | |
| Number of preps | 24 |
| Number of indices provided | 12 (for 24 preps, indexes 1-24 or 25-48) |
| Time required to complete 6 libraries | As little as 5 hours |
| Input RNA required | As little as 50 pg |
Storage Conditions and Product Stability
Some components require storage at -20°C, 4°C or room temperature. See individual components and box labels for storage conditions.
| Step | Component | Cat. 64600 (24 preps) |
|---|---|---|
| 3′ AdaptorLigation to Template RNA | 3′ Adaptor | 30 µL |
| 3′ Adaptor Ligation Master Mix | 320 µL | |
| T4 RNA Ligase 2 (Truncated) | 35 µL | |
| 5′ Adaptor Ligation | 5′ Adaptor | 30 µL |
| 5′ Adaptor Ligation Master Mix | 320 µL | |
| T4 RNA Ligase 1 | 35 µL | |
| cDNA Synthesis from Ligated RNA Product | Reverse Primer | 30 µL |
| cDNA Synthesis Master Mix | 220 µL | |
| TruScript ReverseTranscriptase | 35 µL | |
| PCR Amplification | 2x NGS PCR Master Mix | 1.32 µL |
| PCR Reverse Primer | 81 µL | |
| Forward Index Primer | Included in Small RNA Library Prep Forward Index Primers (# 64640 or # 64610) | |
| Size Selection | NGS MW Ladder | 50 µL |
| NGS Control Ladder | 50 µL | |
| Loading Dye | 300 µL | |
| Nuclease-Free Water | 1.25 µL |
| Component | Cat. 63500 (75 preps) |
|---|---|
| Buffer RL | 40 mL |
| Wash Solution A | 38 mL (Reconstituted to 128 mL) |
| Elution Solution A | 6 mL |
| Columns | 75 |
| Gel Filtration Columns | 24 |
| Collection Tubes | 75 |
| Elution Tubes | 75 |
| Product Insert | 1 |
Overview
This kit provides a fast and simple procedure for the isolation of total proteins from tissue, bacteria, yeast or mammalian cells without the use of SDS, Triton® X-100 and other detergents. Detergents are extensively used to prepare protein samples; however, these detergents have undesirable effects on downstream analysis. These effects include extraneous peaks in mass spectrometry, artifacts with chromatography and electrophoresis, interference with microinjection into cells and interference with protein immunization.
The Detergent-Free Total Protein Isolation Kit maintains high protein recovery and yields proteins that are 100% detergent-free. Purification is based on using Norgen’s proprietary resin together with Lysis Solution, followed by protein filtration using a filter column (provided). The purified proteins can be used in a number of downstream applications including mass spectrometry, SDS-PAGE, isoelectric focusing, NMR spectroscopy and more.
Each Lysis Tube is able to process up to 50 mg of tissue, 1010 bacterial cells, 109 yeast cells or 107 mammalian cells. Preparation time for 12 samples is less than 10 minutes.
Figure 1 / 1
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| Kit Specifications | |
| Maximum Amount Of Starting Material: Tissues Animal Cells Bacteria Yeast | 50 mg 1 x 107 cells 1 x 1010 cells 1 x 109 cells |
| Time to Process 12 Samples | Less than 10 minutes |
Storage Conditions and Product Stability
The Lysis Solution should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 30300 (50 preps) |
|---|---|
| Lysis Solution | 4 mL |
| Lysis Tubes | 25 |
| Filter Columns | 25 |
| Elution Tubes (1.7 mL) | 25 |
| Product Insert | 1 |