The Hepatitis B virus (HBV) is mainly transmitted via blood or blood products. In addition, sexual, oral and perinatal infections are also possible. Early symptoms of the infection include appetite loss, vomiting and abdominal symptoms, with approximately 10-20% of those patients developing fever as well as rheumatoid joint and muscle pain. Jaundice, which may be accompanied by itching, will then develop within 2-14 days. Fulminant hepatitis then occurs in about 1% of all infected patients, which in severe cases may be fatal. Of those individuals infected by HBV, 5-10% will develop chronic liver inflammation which may progress to cirrhosis of the liver or, in the worst case, primary liver cell carcinoma. The HBV virus is a hepadnavirus that has a circular genome composed of partially double-stranded DNA.
HBV TaqMan PCR Kit, 100 reactions
HBV TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM29250 (100 rxns) | Cat. TM29210 (100 rxns) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| HBV Primer & Probe Mix | 280 μL | 280 μL |
| HBV Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Usages:
For selective isolation and culture of Pseudomonas aeruginosa.
Principle:
Acetamidase bacteria can use acetamide as a carbon source and acetamide decomposition Alcaligenes; NaCl maintain osmotic balance; dipotassium phosphate and potassium dihydrogenphosphate as buffer; magnesium ions provide necessary growth ; agar medium as coagulant; phenol red as a pH indicator.
Formulation:
Acetamide 10g
NaCl 5g
Dipotassium hydrogen phosphate 1.39g
Potassium dihydrogen phosphate 0.73g
Magnesium sulfate 0.5g
Phenol red 0.012g
Agar 15g
Final pH 7.2 ± 0.2
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
100g
