1 kb Plus DNA Ladder in 1% agarose gel.
• For sizing and quantification of double strand DNA fragments.
• Composed of 13 bands as shown on right.
• The 10 kb and 4 kb bands with higher concentration are easily distinguishable from the others.
• Premixed with 6X DNA loading buffer for direct gel loading.
1 kb Plus DNA Ladder in 1% agarose gel.
• For sizing and quantification of double strand DNA fragments.
• Composed of 13 bands as shown on right.
• The 10 kb and 4 kb bands with higher concentration are easily distinguishable from the others.
• Premixed with 6X DNA loading buffer for direct gel loading.
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples. Up to 100mg stool sample and 500 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed inparallel.
Specifications
| Features | Specifications |
| Main Functions | Isolation DNA from 200-500mg soil, 50-100mg stool, or 100-500mg other environmental samples using 96 plate |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification method | 96 well plate |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Soil, stool, other environmental samples |
| Sample amount | Soil: 200-500mgStool: 50-100mgOther environmental samples: 100-500mg |
| Elution volume | |
| Time per run | |
| Liquid carrying volume per column | |
| Binding yield of column |
Soil/Stool sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our proprietary Absorber Solution. Binding conditions are then adjusted and the sample is applied to a DNA Mini Column. Two rapid wash steps remove trace contaminants and pure DNA is eluted in low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.
Kit Contents
| Contents | D314401 | D314402 | D314403 |
| Purification Times | 1 x 96 Preps | 4 x 96 Preps | 20 x 96 Preps |
| HiPure DNA Plate | 1 | 4 | 20 |
| 96 Well Plate (2.2ml) | 1 | 4 | 20 |
| 1.6ml Collection Plate | 1 | 4 | 20 |
| 0.8ml Collection Plate | 1 | 4 | 20 |
| 2ml Bead Tubes | 100 | 400 | 2000 |
| Buffer SOL | 100 ml | 360 ml | 2 x 900 ml |
| Buffer SDS | 10 ml | 36 ml | 180 ml |
| Reagent DX | 1 ml | 1.8 ml | 9 ml |
| Buffer PS | 20 ml | 80 ml | 400 ml |
| Absorber Solution | 20 ml | 80 ml | 400 ml |
| Buffer GDP | 150 ml | 500 ml | 3 x 900 ml |
| Buffer GW2* | 50 ml | 100 ml | 4 x 200 ml |
| Buffer AE | 30 ml | 120 ml | 500 ml |
Storage and Stability
Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples. Up to 100mg stool sample and 500 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed inparallel.
6-Formyl-2-pyridine-PEG4-DBCO is a bifunctional PEG linker featuring an aldehyde and a DBCO function. DBCO is a strained cyclooctyne which reacts with azide groups on target molecules while the aldehyde group is a versatile electrophile that is reactive towards amines, hydroxylamines, and hydrazines.
6-Formyl-2-pyridine-PEG4-DBCO is a bifunctional PEG linker featuring an aldehyde and a DBCO function. DBCO is a strained cyclooctyne which reacts with azide groups on target molecules while the aldehyde group is a versatile electrophile that is reactive towards amines, hydroxylamines, and hydrazines.