This kit is designed for the rapid spin column preparation of genomic DNA from 2 x 109 viable bacterial cells (between 0.5 and 1.0 mL of culture). This kit can be used for both Gram-negative and Gram-positive bacteria including Escherichia coli and Bacillus cereus. Purified genomic DNA is of an excellent quality and yield, and is fully compatible with restriction enzyme digestions, sequencing, PCR, qPCR and more. Also available in 96-well format for high throughput applications.
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| Kit Specifications (96-well) | |
| Maximum Input | 2 x 109 bacterial cells |
| Well Binding Capacity | 25 µg |
| Average Yield* | Up to 20 µg* |
| Time to Complete 10 Purifications | 90 minutes |
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
| Component | Cat. 17900 (50 preps) | Cat. 17950 (192 preps) |
|---|---|---|
| Resuspension Solution A | 20 mL | 60 mL |
| Lysis Buffer P | 18 mL | 60 mL |
| Solution BX | 28 mL | 110 mL |
| Wash Solution A | 18 mL | 2 x 38 mL |
| Elution Buffer B | 30 mL | 2 x 30 mL |
| Proteinase K | 12 mg | 50 mg |
| Spin Columns | 50 | – |
| 96-Well Plate | – | 2 |
| Adhesive Tape | – | 4 |
| Collection Plate | – | 2 |
| Collection Tubes | 50 | – |
| Elution Tubes (1.7 mL) | 50 | – |
| Elution Plate | – | 2 |
| Product Insert | 1 | 1 |
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Specifications
| Features | Specifications |
| Main FunctionsC | Co-isolation DNA and RNA from a single FFPE tissue sample |
| Applications | RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | FFPE slice, FFPE embedded tissue |
| Sample amount | No more than six 10µm sections of 150mm2 surface area or three 20µm sections of 150mm2 surface area. |
FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. For RNA purification, transfer RNA Lysate to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-salt buffer. For DNA purification, transfer DNA Lysate to an adsorption column and DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA was finally eluted with low-salt buffer.
1.Use non-toxic dewaxing solution without contact with xylene
2.Obtain both DNA and RNA simultaneously from the same sample. Elute separately without affecting each other (Have the same steps and effects as top brand 80234, perfect substitute.)
| Contents | IVD5116 |
| Purification Times | 50 Preps |
| HiPure DNA Micro Column | 50 |
| HiPure RNA Mini Column I | 50 |
| 2ml Collection Tubes | 150 |
| Proteinase K | 50 mg |
| Protease Dissolve Buffer | 5 ml |
| Buffer DPS | 60 ml |
| Buffer FRL | 15 ml |
| Buffer ATL | 15 ml |
| Buffer RLC | 15 ml |
| Buffer AL | 15 ml |
| Buffer VHB | 44 ml |
| Buffer RW2 | 25 ml |
| RNase Free Water | 10 ml |
| Buffer AE | 10 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Propargyl-PEG3-t-butyl ester consists of an alkyne group and a t-butyl ester group. The alkyne groupreacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The protected carboxyl group can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG3-t-butyl ester consists of an alkyne group and a t-butyl ester group. The alkyne groupreacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The protected carboxyl group can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.