8x15ml Angle Rotor

Overview

• Genomic DNA can be isolated from as few as 10 bacterial cells in 1 mL of milk
• Isolate genomic DNA from both Gram-negative and Gram-positive bacteria in milk
• Can process challenging samples such as mastitic milk
• Inhibitor-free DNA is ready for PCR, qPCR, Southern Blot, sequencing & more
• Fast and efficient spin-column format

This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples.  It can also be used to process challenging milk samples such as Mastitic milk for example.  The kit is highly sensitive and can isolate DNA from a cell density of as little as 10 bacteria contained in 1 mL of milk.  Genomic DNA is isolated in 45 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more.​

Details

Supporting Data

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Kit Specifications
Maximum Milk Input	1 mL
Time to Complete 10 Purifications	1 hour
DNA Yield*	500 ng to 8 μg
Bacteria Species Processed	Gram positive and Gram negative
Minimum Detection Limit	10 bacteria in 1 mL of milk

*The range of the DNA yield will vary depending upon a number of factors including bacterial species and type of milk (fat content and %)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year from the date of shipment.

Component	Cat. 21550 (50 preps)
Resuspension Solution A	6 mL
Buffer SK	60 mL
Wash Solution A	18 mL
Elution Buffer B	15 mL
Proteinase K	12 mg
Lysozyme (powder)	120 mg
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

DBCO-amine is a simple building block containing a DBCO moiety and will add minimal spacer to the modified molecules. In the presence of activators such as EDC or HATU, this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-amine is a simple building block containing a DBCO moiety and will add minimal spacer to the modified molecules. In the presence of activators such as EDC or HATU, this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Ready-to-use
• Highly stable
• Precise
• 22 discrete bands from 60 bp to 600 bp

• High quality ready-to-use double-stranded DNA ladder derived by recombinant technology for precise size and mass determination in various fluorescence-detection instruments including different models of Applied Biosystems® PRISM® and Genetic Analyzers 
• 22 discrete bands, ranging from 60 bp to 600 bp
• The ladder is asymmetrically labelled with ROX that can be detected by excitation at 576 nm and fluorescent emission at 597 nm
• Compatible with products for fragment analysis including Promega PowerPlex® 16 System

Details

Supporting Data

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Ladder Properties:
• 22 discrete bands, ranging from 60 bp to 600 bp
 

Storage:
• Store at -20°C in small aliquots. For longer term storage, -70°C is recommended.
• The product is light-sensitive. Minimize light exposure.