Product Description
Highly Sensitive Isothermal Amplification Kit with Fast Reaction Time
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3′ end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | RNA Isothermal Amplification Kit NFO |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal nucleic acid Applications
Suitable for RNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
K-LARGE
SKU: 700004309
50 Assays per kit
| Content: | 50 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 1 year under recommended storage conditions |
| Analyte: | Ammonia, L-Arginine, Nitrogen, Urea, YAN |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Decrease |
| Linear Range: | 1.0 to 35 mg of L-arginine, 0.2 to 7.0 μg of ammonia and 0.3 to 14 µg of urea per assay |
| Limit of Detection: | 0.07 mg/L (ammonia), 0.13 mg/L (urea), 0.37 mg/L (L-arginine) |
| Reaction Time (min): | ~ 20 min [ammonia (2 min), urea (6 min), L-arginine (7 min)] |
| Application examples: | Grape juice, wine must, wine and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Improved method |
The L-Arginine/Urea/Ammonia test kit is specific and a rapid measurement and analysis of L-arginine, urea and ammonia in grape juice/must and wine.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Display all of our nitrogen assay kit products.
Advantages
The L-Arginine/Urea/Ammonia test kit is specific and a rapid measurement and analysis of L-arginine, urea and ammonia in grape juice/must and wine.
Propargyl-PEG3-bromide comprises a propargyl group and a bromide group. The propargyl group reacts with azide-bearing compounds or biomolecules in Click Chemistry to yield a stable triazole linkage; copper will be needed as a catalyst. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG3-bromide comprises a propargyl group and a bromide group. The propargyl group reacts with azide-bearing compounds or biomolecules in Click Chemistry to yield a stable triazole linkage; copper will be needed as a catalyst. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.