Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
Specifications
| Features | Specifications |
| Concentration | 100 mg/ml |
| Appearance | Suspension of black particles |
| Surface functional group | Si-OH, Silanol |
| Dispersibility | Polydisperse Amorphous |
| Particle size | 1.5-5 μm |
| Preservation conditions | Room Temperature, valid for up to 2 years.It is recommended to store in 2-8°C to prevent microbial growth. |
| Magnetic response speed | 15-30 seconds |
| Settling velocity | >5 minutes |
| High salt mediated binding | >2M guanidine isothiocyanate, DNA recovery up to 80% |
| Alcohol mediated binding | 2M guanidine hydrochloride / isopropanol (30%), and the recovery of DNA / RNA was as high as 85% |
| PEG8000 mediated binding | The recovery of DNA/RNA was up to 85% |
| DNase/RNase | Not detected |
| DNA residue | <1 ppm |
| Recommended application | Plasmid extraction, gel DNA recovery, genomic DNA extraction and RNA extraction. |
Principle
Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.
Alcohol mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.
After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.
Ordering information
| CAT.No. | Product Name | Package |
| C14100 | MagPure Particles | 100 ml |
| C14101 | MagPure Particles | 400 ml |
| C14102 | MagPure Particles | 3 x 400 ml |
| C14103 | MagPure Particles | 10 x 400 ml |
| Features | MagPure Particles | MagPure Particles N | MagPure Particles G | MagPure Particles F | MagBind Particles |
| Cat.No. | C1410 | C1411 | C1412 | C1414 | C1413 |
| Concentration | 100mg/ml | 70mg/ml | 40mg/ml | 50mg/ml | 10mg/ml |
| Form | Amorphous and Porous | Amorphous and Porous | Porous | Amorphous | Nonporous |
| Surface function | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | COOH, Carboxyl Beads |
| Dispersion | Polydisperse | Polydisperse | Monodisperse | Monodisperse | Monodisperse |
| Particle Size | 1.5-5μm | 0.2-2μm | 1-1.5μm | 0.2-1.5μm | 0.8-1μm |
| Color | Black | Yellowish Brown | Dark Brown | Dark Brown | Yellowish Brown |
| Magnetic response | 15-30s | ~60s | ~30s | 20s | 120s |
| Settling Time (1ml) | >5min | >10min | >3min | >3min | >2h |
| Usage (0.2ml Sample) | 20μl | 20μl | 20-30μl | 20-30μl | 20-30μl |
| DNA Recover Rate (only 4M GITC) | >80% | >80% | >80% | >80% | 0 |
| DNA Recover Rate (10% PEG8000/NaCl) | >85% | >85% | >85% | >85% | >90% |
| Recommended Use | gDNA/RNA Isolation from Blood, Tissue, Plant, Swab, Spots, Stool, Soil and etc.Viral DNA/RNA IsolationAgarose Gel DNA Purification | DNA/RNA Isolation from low nucleic acid content samplesPlasmid IsolationDNA/RNA Clean Up | Circulating DNA IsolationViral Nucleic acid IsolationgDNA Isolation FFPE DNA/RNA Isolation | Plasmid extractiongel DNA recoverygenomicDNA/RNA extraction viral nucleic acid extractionCirculating DNA extraction | DNA/RNA Clean Up and concentrationDNA/RNA Isolation from low nucleic acid content samplesResearch immuno assays |
Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
Norgen’s FBS Exosome Depletion Kits provides a quick and easy protocol for the depletion of bovine exosomes from FBS prior to using it as a growth supplement in your culture medium. The FBS recovered from the depletion process is exosome-depleted and does not contain any quantifiable bovine miRNAs. Moreover, the exosome-depleted FBS will support the growth of your cells of interest similar to the non-depleted FBS. Norgen’s kits allow for the processing of different FBS volumes. The depletion is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available kits in that they do not require ultracentrifugation, any special instrumentation, precipitation reagents or any protease treatments. More importantly, the depletion process is an inexpensive method for exosome depletion from FBS, as compared to the expensive current ready-to-use exosome-depleted media available on the market.
Background
Most culture medium used for the growth and propagation of cells in culture require the addition of fetal bovine serum (FBS) as a growth supplement to media. FBS is obtained from bovine (cow) serum, and therefore contains large quantities of bovine exosome vesicles. These exosomes may interfere with some types of studies, or may lead to unreliable results when studying the exosomes shed from your cells of interest in normal culture conditions. Therefore, the use of exosome-depleted FBS is highly recommended for many types of studies.
FBS Exosome Depletion Kit (Slurry Format)
For FBS volumes ranging from 140 mL to 280 mL.
FBS Exosome Depletion Kit (Column Format)
For FBS volumes ranging from 120 mL to 240 mL.
Figure 1 / 3
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| Kit Specifications | |
| Sample Type | Fetal Bovine Serum |
| Sample Volume Range | Up to 140 mL (FBS Exosome Depletion Kit I (Slurry Format) Up to 280 mL (FBS Exosome Depletion Kit II (Slurry Format) |
| Depletion | Deplete exosome-sized vesicle |
| Bovine miRNA | No detectable bovine miRNA |
| Time to Complete 6 Purifications | 40 minutes |
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 61100 (6 preps) | Cat. 61400 (12 preps) | Cat. 61200 (6 preps) | Cat. 61300 (12 preps) |
|---|---|---|---|---|
| ExoC Buffer | 2 x 1.5 mL | 8 mL | 2 x 1.5 mL | 8 mL |
| Slurry E | 12.5 mL | 2 x 12.5 mL | – | – |
| Maxi Spin Column | – | – | 6 | 12 |
| Product Insert | 1 | 1 | 1 | 1 |
As this is a 2 gene kit, we recommend purchase of 2 of the accompanying RT-qPCR master mix reagent: oasig Lyophilised OneStep RT-qPCR Master Mix 150 reactions.
Norovirus is known to cause acute gastroenteritis. It is a small (27-38 nm), round, nonenveloped RNA virus belonging to the Caliciviridae family and is responsible for over 80% of non-bacterial outbreaks of gastroenteritis in the world. It affects individuals of all ages, with a distinct seasonal link to winter. It has a genome of 7.6 kb that is positive sense and has a single stranded linear confirmation. It encodes a major structural protein (VP1) of about 58 to 60 kDa and a minor capsid protein (VP2). Transmission occurs predominantly through ingestion of contaminated water, food and airborne transmission, as well as contact with contaminated surfaces. The ease with which norovirus is transmitted and the low infectious dose required to establish an infection results in extensive outbreaks in numerous environments, such as hospitals, hotels and schools. There is no antiviral drug available to treat this infection and little is known about its pathogenicity. However, it has been observed that the virus can be taken up by enterocytes where translation of viral nonstructural proteins can occur; it damages and alters intestinal microvilli, leaving them blunt and broadened, thus inhibiting absorption; it causes crypt cell hyperplasia and also leads to apoptosis of enterocyctes. An incubation period of 24-48 hours is usual. Infection is characterized by the acute onset of nausea, vomiting, abdominal cramps, aching limbs, raised temperature and diarrhoea that generally last for about 48 hours. However, more severe and prolonged infection may be observed in children and the elderly. There are five recognized norovirus genogroups, of which three (GI, GII, and GIV) are known to affect humans and, since 2002, variants of the GII.4 genotype have been the most common cause of norovirus outbreaks. There have been 31 different genotypes identified within the genogroups, with a wide degree of genetic variability present even within each genotype.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings