This product is suitable for extracting total pathogen RNA from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified RNA can be used for clinical in vitro detection.

It is special designed for Pathogen RNA extraction from Sputum samples, can be used in tuberculosis (TB) detection.

Details

Specifications

Features	Specifications
Main Function	Extract total pathogen RNA from cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant, special design for sputum samples. Used in tuberculosis detection.
Applications	RT-PCR，PCR
Products	Pathogen RNA
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant, sputum
Sample amount	200-300μl
Adaptive instrument	Nucleic acid extractor, pipetting workstation

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.

Advantages

• Fast – several samples can be extracted in 40 minutes by column method
• High quality – high purity total RNA / DNA can be directly used in various sensitive downstream applications
• Safe – no phenol chloroform extraction required
• Sensitive – DNA/RNA can be recovered at the level of PG

Kit Contents

Contents	IVD6672C
Purification Times	200 Preps
2ml Bead Tube	4 x 50
MagPure Particle	7.0 ml
Proteinase K	100 mg
Protease Dissolve Buffer	6 ml
DTT （Powder)	2g
Buffer SDS	15 ml
Buffer MLBN	220 ml
DNase I	4 x 0.6 ml
DNase Buffer	60 ml
Buffer MW1*	53 ml
Buffer MW2*	50 ml
Buffer NFW	30 ml

Storage and Stability

MagPure Particles and Proteinase K, DNase I  should be stored at 2–8°C upon arrival. However, short-term storage (up to 2 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.

This product is suitable for extracting total pathogen RNA from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified RNA can be used for clinical in vitro detection.

N-(Propargyl-PEG2)-N-bis(PEG1-alcohol) is a multi-arm linker that can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

N-(Propargyl-PEG2)-N-bis(PEG1-alcohol) is a multi-arm linker that can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

K-DMAL

100 assays (manual) / 1000 assays (microplate) / 1100 assays (auto-analyser)

Content:	100 assays (manual) / 1000 assays (microplate) / 1100 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	D-Malic Acid
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	0.5 to 40 µg of D-malic acid per assay
Limit of Detection:	0.26 mg/L
Reaction Time (min):	~ 6 min
Application examples:	Wine, beer, fruit juices, soft drinks, dietetic foods, candies, fruit and vegetables, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Methods based on this principle have been accepted by EEC, EN, DIN, OIV, IFU, and AIJN

This product has been discontinued (read more).

The D-Malic Acid assay kit is suitable for the specific measurement and analysis of D-malic acid (D-malate) in beverages and food products.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

Interested in more assay kits? See our complete list of organic acid assay kits.

Advantages

• Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4^oC.
• No wasted D-malate dehydrogenase solution (stable suspension supplied) 
• Very competitive price (cost per test) 
• All reagents stable for > 2 years after preparation 
• Rapid reaction (even with difficult samples) 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included
• Suitable for manual, microplate and auto-analyser formats

The D-Malic Acid assay kit is suitable for the specific measurement and analysis of D-malic acid (D-malate) in beverages and food products.