The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with our MSP750 MSP750R MSPU650R or MSPU650 magnet plates and is available as a discounted kit
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96-Well 0.2 mL PCR Strip Rack SBS/ SLAS Footprint
SKU: MTP96
The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with our MSP750 MSP750RMSPU650R or MSPU650 magnet plates and is available as a discounted kit
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PACE® TRIAL KITS
Product Info
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Product Info
ABOUT
Everything you need to run a trial PACE® allele-specific PCR Genotyping Reaction on your existing lab equipment. Each PACE Trial Kit includes Test DNA samples, PACE Genotyping Assays, PACE Master Mix and a comprehensive PACE Genotyping Trial Kit Manual.
WHO IS THIS TRIAL KIT FOR?
Anyone who wants to try PACE genotyping reagents in their lab for the first time with a set of validated DNA samples, SNP assays and PACE Master Mix.
TRIAL KIT OVERVIEW
Step 1. Dispense each of the three trial DNA samples (DNA 1, 2 and 3) plus water (No Template Control) in triplicate onto a PCR plate using the suggested volumes.
Step 2. Combine appropriate volumes of PACE Genotyping Master Mix with PACE Genotyping Assay in a tube, as directed, then mix.
Step 3. Dispense the combined mixtures into each of the wells containing DNA using volumes indicated. Each test now contains a complete PACE Genotyping Reaction.
Step 4. Seal your PCR plate with an optically clear seal and centrifuge to ensure all components are at the bottom of the wells.
Step 5.Thermally cycle the reaction plate using the thermal cycling conditions provided.
Step 6. Read the plate and compare data produced with the expected results provided in the manual. Simple!
PACE MECHANISM
More information on the PACE genotyping chemistry and how it works can be found here: www.3crbio.com/#pace. PACE allele-specific PCR is used for the detection of SNPs, Indels and other sequence variants.
REQUIRED COMPONENTS
qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal
Propargyl-PEG6-Ms is a PEG reagent that enables Click Chemistry reactions with azide-bearing compounds or biomolecules; copper is needed as a catalyst. The PEG spacer increases the water solubility of the reagent in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG6-Ms is a PEG reagent that enables Click Chemistry reactions with azide-bearing compounds or biomolecules; copper is needed as a catalyst. The PEG spacer increases the water solubility of the reagent in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
1.5 mL – 2 mL Micro-centrifuge Tube or Screw-Cap Vial Chiller Block
SKU: CB024
Permagen’s SBS/ANSI footprint 24-Tube thermal block allows the use of up to twenty four 1.5 mL -2 mL Microcentrifuge tubes, or screw-cap vials for keeping sample temperatures consistent. Can be used for heating as well
Document
Permagen’s SBS/ANSI footprint 24-Tube thermal block allows the use of up to twenty four 1.5 mL -2 mL Microcentrifuge tubes, or screw-cap vials for keeping sample temperatures consistent. Can be used for heating as well