The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with our MSP750 MSP750R MSPU650R or MSPU650 magnet plates and is available as a discounted kit
SKU: MTP96
The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with our MSP750 MSP750R MSPU650R or MSPU650 magnet plates and is available as a discounted kit
| Clone | IHC411 |
| Source | Rabbit Monoclonal |
| Positive Control | Tonsil, Lung Adenocarcinoma |
| Dilution Range | 1:200 |
Programmed Death-Ligand 1 (PD-L1), also known as CD274 or B7 Homolog 1 (B7-H1), is a transmembrane protein involved in suppressing the immune system and rendering tumor cells resistant to CD8 T cell-mediated lysis through binding of the Programmed Death-1 (PD-1) receptor. Overexpression of PD-L1 may allow cancer cells to evade the actions of the host immune system. In renal cell carcinoma, upregulation of PD-L1 has been linked to increased tumor aggressiveness and risk of death, and, in ovarian cancer, higher expression of this protein has lead to significantly poorer prognosis. PD-L1 has also been linked to systemic lupus erythematosus and cutaneous melanoma. When considered in adjunct with CD8 tumor-infiltrating lymphocyte density, expression levels of PD-L1 may be a useful predictor of multiple cancer types, including stage III non-small cell lung cancer, hormone receptor negative breast cancer, and sentinel lymph node melanoma.
GL20 Technical Parameter：
| Max. Speed | 21000rpm |
| Max. RCF | 30910×g |
| Max. Capacity | 4×800ml |
| Time Range | 1~9h59min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 60 |
| Temperature | -20℃~40℃ |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | ±1℃ |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (W x D x Hmm) | 680×540×830mm |
| Net Weight(Kg) | 170KG |
| Certificates | CE,ISO & Calibration report are available |
Matched Rotors for GL20
| Order No | Rotor | Rotor Max Speed(r/min) | Volume(ml) | RCF(×g) |
| G20-1 | Swing Rotor | 4000 | 4×800ml | 3450 |
| G20-2 | Microplate rotor | 4000 | 4×4×96 well | 2940 |
| G20-3 | Microplate rotor | 4000 | 2×4×96 well | 3210 |
| G20-4 | Microplate rotor | 4000 | 2×3×48 well | 2300 |
| G20-5 | Swing rotor | 4000 | 4×40×7ml vacuum tube | 3270 |
| G20-6 | 4000 | 4×40×5ml vacuum tube | 2890 | |
| G20-7 | 4000 | 4×28×10ml vacuum tube | 3130 | |
| G20-8 | Swing Rotor | 4000 | Fat bottle | 3830 |
| G20-9 | Fixed Rotor | 16000 | 4×8PCR | 15760 |
| G20-10 | Fixed Rotor | 15000 | 6×8PCR | 21420 |
| G20-11 | Fixed Rotor | 16000 | 8×8PCR | 17480 |
| G20-12 | Fixed Rotor | 15000 | 12×8PCR | 22930 |
| G20-13 | Fixed Rotor | 15000 | 40×0.5ml | 22920 |
| G20-14 | Fixed Rotor | 21000 | 12×1.5ml | 30910 |
| G20-15 | Fixed Rotor | 16000 | 24×1.5ml | 23440 |
| G20-16 | Fixed Rotor | 14000 | 30×1.5ml | 20800 |
| G20-17 | Fixed Rotor | 13000 | 48×1.5ml | 17930 |
| G20-18 | Fixed Rotor | 16000 | 16×5ml | 22020 |
| G20-19 | Fixed Rotor | 16000 | 6×10ml | 21500 |
| G20-20 | Fixed Rotor | 15000 | 12×10ml | 22680 |
| G20-21 | Fixed Rotor | 13000 | 16×10ml | 19490 |
| G20-22 | Fixed Rotor | 13000 | 8×15ml | 17790 |
| G20-23 | Fixed Rotor | 11000 | 12×15ml | 14330 |
| G20-24 | Fixed Rotor | 5000 | 24×15ml | 3500 |
| G20-25 | Fixed Rotor | 5000 | 30×15ml | 3830 |
| G20-26 | Fixed Rotor | 14000 | 6×30ml | 19060 |
| G20-27 | Fixed Rotor | 13000 | 6×50ml(conical) | 18840 |
| G20-28 | Fixed Rotor | 13000 | 6×50ml(round) | 18730 |
| G20-29 | Fixed Rotor | 5000 | 12×50ml | 3860 |
| G20-30 | Fixed Rotor | 4000 | 24×50ml | 2970 |
| G20-31 | Fixed Rotor | 13000 | 4×85ml | 18940 |
| G20-32 | Fixed Rotor | 12000 | 4×100ml | 14850 |
| G20-33 | Fixed Rotor | 10000 | 6×100ml | 11380 |
| G20-34 | Fixed Rotor | 4000 | 12×100ml | 2970 |
| G20-35 | Fixed Rotor | 12000 | 24 capillaries | 15800 |
| G20-36 | Swing Rotor | 15000 | 4×5ml | 19920 |
| G20-37 | Vertical rotor | 16000 | 16×5ml | 16450 |
| G20-38 | Vertical rotor | 14000 | 8×30ml | 19750 |
Features
1. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.
2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control
3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.
4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.
6.High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect inner sleeve , 3 tiers protection,safe and reliable.
7. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.
Sample type purification kit guide
The 16S V3-V4 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V3-V4 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V3-V4 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V3-V4 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V3-V4 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Step | Component | Cat. 70400 (24 preps) | Cat. 70410, 70420, 70430, 70440 (96 preps) |
|---|---|---|---|
| Amplicon PCR (PCR 1) | MGX Master Mix | 330 µL | 1,320 µL |
| 16S V3-V4 Primer Mix | 70 µL | 280 µL | |
| Index PCR (PCR 2) | Indexing Master Mix | 660 µL | 2 x 1,320 µL |
| N7xx Index Primer | 50 µL | 50 µL | |
| S5xx Index Primer | 70 µL | 70 µL | |
| PCR Clean-Up | Resuspension Buffer | 2 x 1,250 µL | 2 x 5,000 µL |
| Nuclease-free water | 1,250 µL | 1 x 6,000 µL |
