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96-Well 0.2 mL PCR Strip Rack SBS/ SLAS Footprint

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The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with our MSP750 MSP750R MSPU650R or MSPU650 magnet plates and is available as a discounted kit

Detail

96-Well 0.2 mL PCR Strip Rack SBS/ SLAS Footprint

SKU: MTP96

The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with our MSP750 MSP750R MSPU650R or MSPU650 magnet plates and is available as a discounted kit

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HCM145 Modified MSRV Agar Base

Introduction

Discover Huankai’s Modified MSRV Agar Base, designed for the selective detection and isolation of motile Salmonella species, ensuring reliable food and environmental testing.

Total RNA Purification Maxi Kit

Overview

  • Extract high quality & quantity total RNA including miRNA
  • No phenol step required – isolate all RNA in one fraction
  • Rapid processing in under 40 minutes
  • Isolate total RNA from a wide variety of specimens
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s Total RNA Purification Maxi Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants, and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Norgen’s Purification Technology

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first lysing the cells or tissue of interest with the provided Buffer RL (please see the flow chart on page 4). Ethanol is then added to the lysate, and the solution is loaded onto a maxi spin column. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. Thus only the RNA will bind to the column, while the contaminating proteins will be removed in the flowthrough or retained on the top of the resin. The bound RNA is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified total RNA is eluted with the Elution Solution A. The purified RNA is of the highest integrity, and can be used in a number of downstream applications.

Details

Supporting Data

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Figure 1. Total RNA Purification Maxi Kit
Figure 1. Total RNA Purification Maxi Kit
Figure 2. Total RNA Purification Maxi Kit

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Kit Specifications
Maximum Column Binding Capacity1.5 mg
Maximum Column Loading Volume20 mL
Size of RNA PurifiedAll sizes, including small RNA
(< 200 nt)
Maximum Amount of Starting Material
Liver Tissue100 mg
Heart Tissue50 mg
Kidney Tissue100 mg
Brain Tissue250 mg
Lung Tissue100 mg
Whole Blood2 – 10 mL*
Bacteria2.5 x 10 10 cells
Yeast2 x 108 cells
Fungi1 g
Plant Tissues1 g
Time to Complete 4 Purifications40 minutes
Average Yield:
HeLa Cells (5 x 107 cells)
E. coli (2.5 x 1010 cells)
~750 μg
~1.5 mg

*For isolating total RNA from purified leukocytes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

ComponentCat. 26800 (8 preps)
Buffer RL2 x 40 mL
Wash Solution A4 x 38 mL
Elution Solution A3 x 20 mL
RNA Maxi Spin Columns (with collection tubes)8
Elution Tubes (50 mL)8
Product Insert1

Raffinose/Sucrose/D-Glucose Assay Kit

K-RAFGL

SKU: 700004332

120 assays of each per kit

Content:120 assays of each per kit
Shipping Temperature:Ambient
Storage Temperature:Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability:> 2 years under recommended storage conditions
Analyte:D-Glucose, Raffinose, Sucrose
Assay Format:Spectrophotometer
Detection Method:Absorbance
Wavelength (nm):510
Signal Response:Increase
Limit of Detection:100 mg/L
Reaction Time (min):~ 20 min
Application examples:Analysis of grain legumes and other materials containing raffinose, stachyose and verbascose.
Method recognition:Used and accepted in food analysis

The Raffinose/Sucrose/D-Glucose test kit is for the measurement and analysis of D-glucose, sucrose and raffinose, stachyose and verbascose in seeds and seed meals. Based on the measurement of D-glucose on enzymic hydrolysis of raffinose, stachyose and verbascose to D-glucose, D-fructose and D-galactose.

Check out our full range of monosaccharide and oligosaccharide assay kits.

Scheme-K-RAFGL RAFGL Megazyme

Advantages

  • Very competitive price (cost per test) 
  • All reagents stable for > 2 years after preparation 
  • Simple format 
  • Rapid reaction 
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
  • Standard included