96-Well 0.2 mL PCR Strip Rack SBS/ SLAS Footprint

Overview

• Compatible with PAXgene Blood RNA Tubes
• Isolate true total RNA including siRNA and microRNA
• No phenol extractions
• Purify high-quality RNA in 30 minutes
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s Preserved Blood RNA Purification Kit II provides a rapid method for the isolation and purification of total RNA from blood that has been preserved using PAXgene Blood RNA Tubes*. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform.

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Norgen’s proprietary resin provides superior affinity to the full size range of RNA molecules, resulting in large and small RNA (miRNA) purification with better linearity and sensitivity. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time RT-PCR, RT-PCR, Northern blotting, RNase protection and primer extension, expression profiling, miRNA cloning and amplification and Next Generation Sequencing.

* PAXgene Blood RNA Tubes are a product of PreAnalytiX GmbH.  PAXgene Blood RNA Tubes are not provided.

Details

Supporting Data

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Kit Specifications
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Time to Complete 10 Purifications	30 minutes
Average Yield	5 – 20 μg per 2.5 mL preserved human blood

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The DNase I should be stored at -20°C.

Component	Cat. 43500 (50 preps)
NPX1	2 x 110
NPX2	40 mL
NPX3	22 mL
NPX4	6 mL
NPX5	6 mL
DNase I	1 vial
Mini Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Features:

1. Programmable microprocessor control system, touch screen indicates rotor No., speed, time, temperature, RCF, rate of speed as well as fault information.  

2. Adopts band heater, fast heating speed, temperature uniform, with the function of temperature-control and constant.

3. Centrifugal tube made in special skill, anti-broken when work.

4. Great toque brushless motor, maintenance-free, no powder, fast acceleration and deceleration, low noise, which improve the stability a lot.

5. Electric lid lock, over-speed and over-temperature protection

6.3 phase to keep the best performance.

7. Stainless steel chamber, safe and reliable

TD5B Technical Parameter：

Max. Speed	4000rpm
Max. RCF	2498×g
Max. Capacity	4×100ml
Time Range	0~99min
Rotor	Swing   Out Rotor
Noise (dB)	≤ 58
Temperature	Room   temperature:+10~80℃
Acc/Dcc	10 Kinds
Speed Accuracy	±20r/min
Temperature   Accuracy	±1℃
Oil tube size	37×200mm
Size (W x D x Hmm)	685×500×385mm
Net Weight(Kg)	67KG

Oil test centrifuge， Heating oil test centrifuge, special tube oil test centrifuge

DBCO-PEG2-PFP ester is a PEG active ester consisting of a DBCO group which can react with azides under copper free conditions. The PFP ester is an active ester which can react with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG2-PFP ester is a PEG active ester consisting of a DBCO group which can react with azides under copper free conditions. The PFP ester is an active ester which can react with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.