96-Well Low Elution PCR side pull bar magnetic plate with integrated cushion base

Overview

• Ready-to-use
• Quantitative
• Precise
• Highly Stable
• Thirteen discrete fragments ranging from 300 bp to 10000 bp
• Higher intensity reference band at 5000 bp

The HighRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains thirteen discrete fragments ranging from 300 bp to 10,000 bp with a higher intensity reference band at 5000 bp. This Ladder is ideal for quick sizing of PCR products and restriction digests.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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HighRanger 1kb DNA Ladder (Cat# 11900) – 100 loads

Ladder Properties:
• Thirteen discrete bands, ranging from 300 bp to 10000 bp
• Higher intensity band at 5000 bp for easy reference

Fragment	Size (bp)	Mass (ng)
1	10000	58
2	8000	53
3	6000	46
4	5000	67
5	4000	56
6	3000	47
7	2500	41
8	2000	34
9	1500	29
10	1000	18
11	700	13
12	500	23
13	300	14

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

This kit is stable for 2 years after the date of shipment.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Introduction

Usages:
For cultivation of bifidobacteria and lactic acid metabolites test.

Principle:
Peptone, yeast extract provide nitrogen, vitamins, and growth factors; glucose as
fermentable sugars; cysteine – HCl, vitamin K1, hemin, magnesium sulfate, calcium
chloride, sodium chloride, sodium bicarbonate training offers various bifidobacteria growth factor; dipotassium hydrogenphosphate, potassium dihydrogenphosphate buffer.

Formulation ( per liter):
Peptone 20g
Glucose 5g
Yeast powder 10g
Cysteine – hydrochloride 0.5g
Calcium chloride 0.008g
Magnesium sulfate 0.008g
Dipotassium hydrogen phosphate 0.04g
Potassium dihydrogen phosphate 0.04g
Sodium bicarbonate 0.4g
Sodium chloride 0.08g
Final pH 6.0 ± 0.2

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

250g

Overview

• RNA/microRNA/DNA/Proteins are preserved for more than 2 years at room temperature in Norgen’s Urine Preservative
• Compatible with most DNA, Total RNA, microRNA and protein isolation methods
• Preservative is available in a single dose liquid format (ampule)
• Preservative is also available in a dried format in tubes – Urine Collection and Preservation Tubes
• Urine Collection and Preservation Devices are available in 4 convenient sizes: 5 cc tubes, 15 cc tubes, 50 cc tubes, and 120 cc cups

Norgen’s Urine Preservative is designed for the rapid preservation of DNA, RNA, microRNA and proteins from fresh urine samples. In addition, the Urine Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins.

Norgen’s Urine Preservative is available in 2 convenient formats:

1.  Urine Preservative Single Dose Ampules
With this product Norgen’s Urine Preservative is supplied in a liquid format in single dose ampules. The user simply collects 5 – 50 mL of urine into a urine collection container and adds the contents of the Urine Preservative Single Dose (Cat# 18126). The urine and preservative are then mixed, and the urine nucleic acids and proteins are preserved at room temperature.

2.  Urine Collection and Preservation Tubes
Norgen’s Urine Preservative is also available in a dried format in Norgen’s Urine Collection and Preservation Tubes. The user simply collects urine into the tubes and mixes gently until the orange preservative pellet in the tube has dissolved. Norgen’s Urine Collection and Preservation Tubes are available in 3 convenient sizes:

• 5 cc tubes, 1 – 5 mL of urine
• 15 cc tubes, 5 – 15 mL of urine
• 50 cc tubes, 5 – 45 mL of urine
• 120 cc cup, 100 – 120 mL of urine

Details

Supporting Data

Figure 1 / 2

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Select Urine Tested with the Norgen Urine Collection and Preservation Tubes

Human
Mouse
Lynx
Wolf
Urine collected from snow

Available Sizes and Formats 

1. Urine Collection and Preservation Cups (120 cc) –  Urine inputs from 100 – 120 mL

Storage Conditions and Product Stability
All tubes/cups should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.

Kit Components	Cat. 18118 (50 tubes)	Cat. 18122 (50 tubes)	Cat. 18113 (50 tubes)	Cat. 18126 (50 tubes)	Cat. 18129 (1 cup)
Urine Collection and Preservation Tubes (5 cc)	50	–	–	–	–
Urine Collection and Preservation Tubes (15 cc)	–	50	–	–	–
Urine Collection and Preservation Tubes (50 cc)	–	–	50	–	–
Urine Preservative Single Dose	–	–	–	50	–
Urine Collection and Preservation Cup (120 cc)	–	–	–	–	1
ID Labels	50	–	–	50	–
Product Insert	1	1	1	1	1