Designed for those labs wishing to separate magnetic beads into rings.
The Permagen ring magnet low elution plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation
Detail
Designed for those labs wishing to separate magnetic beads into rings.
The Permagen ring magnet low elution plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation
SBS SLAS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
The Bisulfite Sequencing Library Prep Kit (illumina platform) was developed for construction of NGS libraries using bisulfite treated DNA (50 ng – 500 ng) as input. DNA methylation is an epigenetic mechanism known to play a critical role in gene regulation and genomic imprinting by blocking transcription factor access to promoters and enhancers. Bisulfite sequencing is a popular technique in biomedical research based on C to T conversion under the treatment of sodium bisulfite.
Recently, NGS became a powerful tool to identify the DNA methylation status at the whole genome level with single-base resolution. However, it is well known that bisulfite treatment of the NGS libraries causes tremendous damage to the libraries.
Bisulfite-Seq kit comparison
In the case of the regular bisulfite seq library preparation (library prep before bisulfite conversion), the DNA shearing equipment and the expensive methylated adaptors are required. In addition, the subsequent bisulfite conversion causes tremendous DNA damage to the constructed libraries.
BioDynami has developed a unique library prep technology to solve the problems. The technology uses bisulfite treated DNA as input to avoid the significant library loss caused by bisulfite conversion. Furthermore, DNA shearing step and expensive methylated adaptors are not required with our kit. The DNA polymerase in the kit has high-fidelity amplification ability and uracil tolerance which is ideal for amplification of bisulfite sequencing libraries. The final library is strand specific.
Bisulfite Sequencing Library Prep Kit Workflow
Three index types are available for the kit:
Non-index (Cat.# 30091): Libraries do not have index.
Index (Cat.# 30092): Each primer contains a unique barcode sequence of 6 bases to identify the individual library. Library multiplexing capacity is up to 48 samples. Index information can be downloaded here.
Unique dual index (Cat.# 30093): The multiplexing of bisulfite sequencing library is up to 96 samples with unique dual indexes. We used a Four-Base Difference Index System to generate indexes that have at least 4 bases different from each other in the 8-base index. The index primers remove NGS errors including index cross-contamination, index hopping, reads mis-assignment etc. Index information can be downloaded here.
Kit advantages
Easy and Quick protocol
Easy: Hands-on time only 10 minutes
Quick: Total protocol time around 1.5 hours
Simple workflow: Less steps
Directional library
Save magnetic beads more than 50%
Guaranteed high Bisulfite sequencing library conversion efficiency
Bisulfite treated DNA as input: From 50 ng to 500 ng
Document
The Bisulfite Sequencing Library Prep Kit (illumina platform) was developed for construction of NGS libraries using bisulfite treated DNA (50 ng – 500 ng) as input. DNA methylation is an epigenetic mechanism known to play a critical role in gene regulation and genomic imprinting by blocking transcription factor access to promoters and enhancers. Bisulfite sequencing is a popular technique in biomedical research based on C to T conversion under the treatment of sodium bisulfite.
Food and beverage samples such as milk or milk based products, wine, fruit juices, paper (and cardboard), waste treatment samples and other materials (e.g. biological cultures etc.).
This product has been discontinued.
The hydrogen peroxide Assay Kit provides a simple robust method for the measurement of hydrogen peroxide. This kit utilizes a highly sensitive Megaplex Red probe which in the presence of horseradish peroxidase (HRP), allows for the measurement of H2O2 in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation in the range 530-560 nm and emission at ~ 590 nm or colourimetrically at 570 nm. The excitation and emission maxima of Megaplex Red are 571 nm and 585 nm respectively.
Fluorometric/UV method for the determination of H2O2 content in a variety of samples.
Advantages
Simple format
Very competitive price (cost per test)
Ability to run in fluorescence or absorbance mode
User friendly – Detailed protocol provided for the creation of calibration curve and the calculation of concentration in fluorescence mode. Single point standard for quicker and simpler analysis in absorbance mode.
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Suitable for manual, microplate and auto-analyser formats
All reagents stable for > 2 years
Document
The hydrogen peroxide Assay Kit provides a simple robust method for the measurement of hydrogen peroxide. This kit utilizes a highly sensitive Megaplex Red probe which in the presence of horseradish peroxidase (HRP), allows for the measurement of H2O2 in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation in the range 530-560 nm and emission at ~ 590 nm or colourimetrically at 570 nm. The excitation and emission maxima of Megaplex Red are 571 nm and 585 nm respectively.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Formaldehyde
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
0.1 to 14 μg of formaldehyde per assay
Limit of Detection:
0.033 mg of formaldehyde per test or 0.016 mg/mL of formaldehyde in a sample treated as per the standard procedure
Limit of Quantification:
0.11 mg of formaldehyde per test or 0.054 mg/mL of formaldehyde in a sample treated as per the standard procedure
Reproducibility (%):
~ 3%
Reaction Time (min):
~ 15 min
Application examples:
Environmental samples, food and beverage samples.
The Formaldehyde Assay Kit provides a simple robust method for the measurement of formaldehyde.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
