96-Well Low Elution Ring Magnetic Plate with Integrated Cushion Base
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Designed for those labs wishing to separate magnetic beads into rings.
The Permagen ring magnet low elution plate with integrated cushion base plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation while the cushion assist compensates for labware inconsistencies.
Detail
Designed for those labs wishing to separate magnetic beads into rings.
The Permagen ring magnet low elution plate with integrated cushion base plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation while the cushion assist compensates for labware inconsistencies.
SBS SLAS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
We will help to establish your experiments using our broad knowledge:
With myPOLS Biotec’s access to large libraries of different mutant polymerases and experience in high throughput screening we can find the best enzyme for your needs.
We also optimize your polymerases for your defined requirements or enhance our DNA polymerase-based products accordingly.
Finally, we can develop freeze-dried enzyme formulations and production.
We at myPOLS Biotec are scientists and have vast experience in polymerase-based product development. You can benefit from this. Get in touch.
Document
We will help to establish your experiments using our broad knowledge:
With myPOLS Biotec’s access to large libraries of different mutant polymerases and experience in high throughput screening we can find the best enzyme for your needs.
We also optimize your polymerases for your defined requirements or enhance our DNA polymerase-based products accordingly.
Finally, we can develop freeze-dried enzyme formulations and production.
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.
Details
Specifications
Features
Specifications
Main Functions
Co-isolation DNA and RNA from FFPE tissue
Applications
RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
Purification method
Polydisperse silicon based magnetic beads (DNA)Monodisperse carbonyl magnetic beads (RNA)
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Adaptive instrument
Nucleic acid extractor and pipetting workstation
Sample type
FFPE slice, FFPE puncture sample, embedded tissue
Sample amount
No more than six 10 µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area
Yield
DNA: 1 – 10 μg, RNA: 1 – 25 μg
Principle
The sample is lysed and digested under the action of lysate and protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, the supernatant contain RNA was collected and bind to MagBind Particles. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Elution Buffer.
Advantages
High quality – high purity total RNA / DNA can be directly used in various downstream applications
Safe – no phenol chloroform extraction required
Simultaneous extraction – simultaneously isolate DNA and RNA from one sample
Post digestion sorting – higher DNA and RNA yields
High RNA yield – monodisperse carbonyl adsorption
Kit Contents
Contents
R632701
R632702
R632703
Purification Times
48 Preps
96 Preps
5 x 96 Preps
MagBind Particles
1.1 ml
2.5 ml
11 ml
MagPure Particles N
1.1 ml
2.5 ml
11 ml
Proteinase K
24 mg
48 mg
220 mg
Protease Dissolve Buffer
3 ml
10 ml
15 ml
Buffer DPS
50 ml
100 ml
2 x 250 ml
Buffer ATL
20 ml
30 ml
120 ml
Buffer BST1
20 ml
40 ml
200 ml
Buffer BXW1*
44 ml
110 ml
3 x 110 ml
RNase Free Water
15 ml
30 ml
120 ml
Storage and Stability
Proteinase K, MagPure Particles N and MagBind Particles should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stablefor at least 18 months under these conditions.
Experiment Data
Document
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.
GL21MC high speed refrigerated centrifuge Application:
Widely used in the area of animal and plant molecular biology, cell biology, clinical applications, bio-engineering, mass preparation and blood, protein precipitation, Separating the biology, viruses, sub-cell, cell and the chemicals in the cell.
Features:
1.Brushless frequency motor in great torque, free maintenance, no carbon dust pollution, quick in speed up and down.
2. Flexible axle driven system which drive the rotor directly, smooth in operation, low noise and small vibration. 3.Import compressors fluorine free, double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control.. 4. Microprocessor control system, the 10 inches LCD display indicates all the parameters in running, 5. There are more than 20 kinds of rotors for your choice, especial the continuous rotors which help you purify a large number of test solution continuously. 6. There are 30 kinds of program and 10 kinds of accelerating and decelerating speed for your choice. 7.ω2t centrifugation is allowed, which make you have the ideal centrifugation result. 8. 20 kinds of rotors for your choice, especial the continuous rotor help you purify a large amount of test solution continuously. 9. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. 10. The centrifuge body is made of high quality steel, safe and reliable. 11. 3 tiers protection steel cover.
Technical parameters:
Max speed
21000r/min
Max RCF
46140*g
Time range
1~99H59min
Speed accuracy
±50r/min
capacity
6*500ml
Noise
≦65dBA
Weight
235KG
Dimension
865*728*1240mm
Packaged weight
285KG
Packaged dimension
1000*850*1510mm
Temperature range
-20~40℃
Temperature accuracy
±1℃
Power
AC110/220V, 50-60HZ,35A
Rotor identification
Automatic identification
Package
wooden box
Matched Rotors for GL21MC:
Order No.
Rotor
Max speed (r/min)
Max capacity (ml)
Max RCF (*g)
30108
Fixed rotor
21000
12*10ml
44440
30117
Fixed rotor
20000
16*10ml
46140
30111
Fixed rotor
20000
6*50ml
44700
30118
Fixed rotor
20000
8*30/20ml
42040
30119
Fixed rotor
15000
8*50ml
27540
30112
Fixed rotor
15000
6*70ml
25480
30120
Fixed rotor
12000
8*100ml
19610
30121
Fixed rotor
11000
4*300ml
18120
30122
Fixed rotor
10000
6*300ml
15730
30123
Fixed rotor
10000
6*500ml
18340
30128
Continuous centrifugal rotor
17000
1000ml
31390
30129
Continuous centrifugal rotor
14000
1800ml
21710
30130
Continuous centrifugal rotor
8000
3000ml
9450
30131
Continuous centrifugal rotor
8000
1800ml
8600
30132
Interval rotor
17000
1000ml
31390
30133
Interval rotor
12000
1200ml
19350
30134
Interval rotor
10000
3000ml
14890
30135
Vertical rotor
20000
16*5ml
38340
Vertical rotor
18000
8*30ml
32650
30180
Swing rotor
15000
4*5ml
21670
30181
Swing rotor
13000
4*30ml
23580
30182
Swing rotor
10000
4*50ml
16240
30694
Bucket rotor
4000
4*4*96holes
2840
30696
Swing rotor
4000
4*500ml
3380
Document
GL21M max. capacity can hold 800ml bottles 4pcs, max speed over 20000rpm
Model:
GL21M