96 Well Plate for Enzyme Assays and Lateral Flow with Detachable Wells 10pack

Overview

• Isolation of exosomal RNA molecules from urine samples
• Rapid and convenient spin-column protocol
• Isolate inhibitor-free urinary microRNA for most downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Optional protocol for purification of exosomal proteins for western blot analysis

This kit provides a rapid spin column procedure for the isolation of exosomal RNA from urine samples. Users can simultaneously concentrate and isolate high quality exosomal RNA, including microRNA, for use in sensitive downstream assays such as RT-PCR, qRT-PCR, NGS, microarrays and more. The protocol can be completed in under 50 minutes. Urine volumes of 1 to 10 mL can be processed easily and rapidly. All sizes of RNA are recovered at an equal rate without the need for using hazardous chemicals like phenol.

Background

Exosomes are 40 – 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascite fluids, among other biological fluids. These vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes which can inform about the cell type from which the exosomes are secreted. For this reason, exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNases they can be efficiently recovered from biological fluids, such as urine.

Details

Supporting Data

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Kit Specifications
Minimum Urine Input	1 mL
Maximum Urine Input	10 mL
Size of RNA Purified	Small exosomal RNA species
Time to Complete Purification	~ 50 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 47200 (50 preps)
Slurry B1	18 mL
Binding Buffer A	20 mL
Lysis Buffer A	2 x 20 mL
Wash Solution A	38 mL
Elution Solution A	6 mL
Mini Filter Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Product Description

Advanced Diagnosis & Treatment Nucleic Acid Detection Shrimp Hemocytoiridescence Virus

Product Description:

The Livestock Disease Kit is a reliable and easy-to-use test kit designed to detect the presence of diseases in livestock, such as cattle, sheep, goats, pigs and horses. The kit has been designed to detect diseases with high accuracy and sensitivity. Also it requires only a small sample volume of 50 μl, making it highly efficient and cost-effective. It is also suitable for a range of canine dog test kit applications, providing fast and accurate results.The reliable detection results make it a reliable and cost-effective solution for farmers and veterinarians looking to diagnose livestock diseases quickly and accurately.

Features:

• Product Name: Shrimp hemocytoiridescence virus isothermal detetction fluorescence kit
• Target Species: Shrimp
• Type: Fluorescence Kit
• Storage Temperature: -20°C
• Sample Type: Serum, Plasma, Whole Blood

Technical Parameters:

Product Name	Shrimp hemocytoiridescence virus isothermal detetction fluorescence kit
Type	Fluorescence Kit
Sample Volume	50 μl
Sample Type	Serum, Plasma, Whole Blood
Storage Temperature	-20°C
Package Size	48 Tests/Kit
Target Disease	Livestock Disease
Target Species	Shrimp

Applications:

Amp-future bio Livestock Disease Kit is an innovative animal health care product designed to detect and diagnose livestock diseases. Developed by a Chinese research team, this kit is a simple and reliable solution to test and treat livestock diseases. With its detection limit of 0.1 ng/ml, users can obtain accurate results quickly and accurately. The test time takes from 5-20 minutes, and the kit typically contains 48 tests/kit. Furthermore, it is easy to store with its cold storage temperature of -20°C.

The Amp-future bio Livestock Disease Kit provides an innovative solution for animal health care. It is an essential product for veterinary professionals, pet owners, and animal health researchers to detect and diagnose livestock diseases. With its simple and reliable detection and diagnosis, this kit is a must-have for anyone who wants to ensure the health of their animals.

Support and Services:

Livestock Disease Kit Technical Support and Services

We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.

• Our technical support staff is available to answer any questions you may have about the product.
• We offer online tutorials and user guides to help you understand the product better.
• We provide technical assistance to help troubleshoot any issues you may be having with the product.
• We offer a warranty on our products.

If you have any questions or need technical support, please do not hesitate to contact us. We are here to help you make the most of your Livestock Disease Kit.

FAQ:

• Q: What diseases can Livestock Disease Kit detect?
  A: Livestock Disease Kit can detect different infectious diseases, such as Foot and Mouth Disease, Bluetongue Disease, Bovine Viral Diarrhea.
• Q: How does Livestock Disease Kit work?
  A: Livestock Disease Kit uses advanced MIRA technology to amplify the target DNA/RNA targent sequences , and detect the disease by analyzing the amplified sequences.
• Q: How long does it take to get results from Livestock Disease Kit?
  A: Livestock Disease Kit can give a result within 5-20 mins.
• Q: What type of sample is suitable for Livestock Disease Kit?
  A: Livestock Disease Kit is suitable for blood, serum, and tissue samples.
• Q: Does Livestock Disease Kit require professional equipment?
  A: No, Livestock Disease Kit does not require any professional equipment,can use with our isothermal equipment or pcr machine

The Livestock Disease Kit is a reliable and easy-to-use test kit designed to detect the presence of diseases in livestock, such as cattle, sheep, goats, pigs and horses. The kit has been designed to detect diseases with high accuracy and sensitivity. Also it requires only a small sample volume of 50 μl, making it highly efficient and cost-effective. It is also suitable for a range of canine dog test kit applications, providing fast and accurate results.The reliable detection results make it a reliable and cost-effective solution for farmers and veterinarians looking to diagnose livestock diseases quickly and accurately.

Introduction

Usages:
For enriching and cultivating Clostridium.

Principle:
Meat extract powder, peptone provide nitrogen; yeast extract powder, cysteine ​​hydrochloride, sodium acetate offers a variety of vitamins and growth factors; glucose carbon source; low concentrations of soluble starch byproduct of metabolic detoxification; sodium chloride maintain osmotic equilibrium; agar coagulant.

Formulation（per liter）:
Beef extract            10g
Peptone                 10g
Yeast extract           3g 
Glucose Monohydrate     5g            
Sodium chloride         5g
Soluble starch           1g
Cysteine Hydrochloride 0.5g
Sodium Acetate          3g
Agar                          0.5g            
Final pH                6.8±0.2

How to use：
1.Suspend 38g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min.
2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Specifications: 250g/bottle 

250g