DBCO-PEG1-NHS Ester is a PEG containing molecule containing an NHS ester moiety that can react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-PEG1-NHS Ester is a PEG containing molecule containing an NHS ester moiety that can react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Usages: For selective isolation of Gram-negative bacteria, especially for Shigella and Salmonella.
Principle: Yeast extract powder provide nitrogen, vitamins, growth factors; sodium chloride to maintain osmotic equilibrium ferric ammonium citrate of iron salts to produce a black iron sulfide; agar as medium coagulant; phenol red as pH indicator.
Formulation（per liter）: Xylose 3.50g L-Lysine 5.00g Lactose 7.50g Sucrose 7.50 g Sodium Chloride 5.00 g Yeast Extract 3.00g Sodium Desoxycholate 2.50g Sodium Thiosulphate 6.80g Fe-Ammonium Citrate 0.80g Phenol Red 0.08g Agar 13.50 Final PH 7.4±0.2
How to use: 1.Suspend 55.2g in 1Lof distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Do not autoclave ,cool to 50℃ and pour into sterile petri dishes. 2.Dilluted and treated samples.
Quality control: Quality control strains were inoculated ,and cultured at 36 ± 1 ℃ for 24h ,results show as follows: strain name strain code growth feature Arizona bacteria CMCC (B) 47001 good black colonies Salmonella typhimurium CMCC (B) 50115 good black colonies Streptococcus faecalis CMCC32223 prohibited —
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Kit
Product Info
Document
Product Info
Overview
Ensure optimal results for sensitive applications like NGS
Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
The purified exosomal RNA is free from any circulating RNA-binding proteins.
No phenol extractions, Proteinase K treatment, nor carrier RNA.
No time-consuming ultracentrifugation, filtration nor special syringes are required.
Concentrate isolated RNA into a flexible elution volume ranging from 50 μL to 100 μL.
Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary separation matrix.
Norgen’s EXTRAClean Plasma/Serum Exosome and Free-Circulating RNA Isolation Kit constitutes an all-in-one system for the purification of exosomes and the sequential isolation of RNA and free-circulating RNA from different plasma/serum sample volumes ranging from 50 μL and up to 10 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or any protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, NGS application, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields
Variable depending on specimen
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
