This product uses an improved salt precipitation purification method to provide a safe and economical solution for High Weight genomic DNA extraction from blood samples, tissue samples, cultured cells, oral swabs, bacteria, and other samples. The extraction does not require the use of toxic phenol chloroform or any expensive reagents, making it the most economical reagent kit for nucleic acid extraction at present. This kit has no limit onthe amount of sample used and can flexibly adjust various amounts of samples. The obtained DNA can be directly used for experiments such as PCR, enzyme digestion, Southern hybridization, and the Third-generation sequencing.
Principles
SolPure DNA Kits is an improved salt precipitation purification method. (Blood samples are lysed in red blood cell lysis buffer to remove red blood cells, and white blood cells are collected by centrifugation.) After lysis, DNA is released into the lysis buffer. RNA is removed by RNASE A. High salt solution is added to precipitate proteins and impurities. Centrifuge to remove precipitate and obtain supernatant containing only DNA. Add isopropanol to precipitate and recover DNA. Wash with 70% ethanol to remove salt, and finally add Buffer TE to dissolve DNA.
Contents | D3317-01 | D3317-02 | D3317-03 |
Purification Times | 10 | 50 | 250 |
10 x Buffer RBC | 4 ml | 50 ml | 100 ml |
Buffer STE | 60 ml | 250 ml | 2 x 550 ml |
Buffer SDS (20%) | 6 ml | 25 ml | 100 ml |
Buffer PPS | 20 ml | 90 ml | 400 ml |
Proteinase K | 12 mg | 50 mg | 240 mg |
Protease Dissolve Buffer | 1.8 ml | 10 ml | 20 ml |
RNase A | 5 mg | 20 mg | 60 mg |
Buffer TE | 10 ml | 60 ml | 250 ml |
RNase A and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
This product uses an improved salt precipitation purification method to provide a safe and economical solution for High Weight genomic DNA extraction from blood samples, tissue samples, cultured cells, oral swabs, bacteria, and other samples. The extraction does not require the use of toxic phenol chloroform or any expensive reagents, making it the most economical reagent kit for nucleic acid extraction at present. This kit has no limit onthe amount of sample used and can flexibly adjust various amounts of samples. The obtained DNA can be directly used for experiments such as PCR, enzyme digestion, Southern hybridization, and the Third-generation sequencing.
MagPure Seed DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and seed. This kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo. Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridizationand transgenosis detection.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from 50-100 mg easy-grinded plant (tender leaf) and seed |
Applications | PCR, transgene detection, fluorescence quantitative PCR, southern blot, SNP site analysis, etc. |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | Conventional economic plant samples |
Sample amount | 50-100mg |
Elution volume | ≥50μl |
Time per run | ≤60 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Kit Contents
Contents | D635201 | D635202 | D635203 |
Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
MagPure Particles | 1.7 ml | 4 ml | 18 ml |
Buffer SOL | 40 ml | 90 ml | 500 ml |
Buffer SDS | 4 ml | 9 ml | 50 ml |
Buffer MPB | 40 ml | 70 ml | 350 ml |
Buffer GW1 | 26 ml | 53 ml | 220 ml |
Elution Buffer | 10 ml | 30 ml | 120 ml |
Storage and Stability
MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
MagPure Seed DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and seed. This kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo. Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridizationand transgenosis detection.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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