96×10ml Swing Buckets Rotor

Overview

• Ensure optimal results for sensitive applications like NGS
• Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
• Purification and enrichment of intact urinary exosomes for functional studies.
• Bind and elute all RNA irrespective of size or GC content, without bias.
• Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
• No phenol extractions, Proteinase K treatment, nor carrier RNA required.
• No time-consuming ultracentrifugation, filtration nor special syringes are required.
• No precipitation reagents nor overnight incubation required.
• Compatible with urine from any species.
• Pure exosomes are purified and are free-from any other RNA-binding proteins.
• Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix.

Norgen’s EXTRAClean Urine Exosome Purification and RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of RNA from different urine sample volumes ranging from 250 μL to 1 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, NGS application, Northern blotting, RNase protection and primer extension, and expression array assays.

Details

Supporting Data

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Minimum Urine Input	250 μL
Maximum Urine Input	1 mL
Size of Exosomes Purified	40 nm – 150 nm
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35-40 minutes
Average Yields	Variable depending on specimen

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

endo-BCN-PEG2-alcohol is a click chemistry PEG linker. The BCN group is very reactive with azide-tagged molecules. The hydroxyl group enables further derivatization or replacement with other reactive functional groups. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

endo-BCN-PEG2-alcohol is a click chemistry PEG linker. The BCN group is very reactive with azide-tagged molecules. The hydroxyl group enables further derivatization or replacement with other reactive functional groups. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

The Primerdesign™ genesig® Kit for Enterocytozoon bieneusi (E. bieneusi) genomes is designed for the in vitro quantification of E. bieneusi genomes. The kit is designed to have the broadest detection profile possible whilst remaining specific to the E. bieneusi genome. The primers and probe sequences in this kit have 100% homology with a broad range of E. bieneusi sequences based on a comprehensive bioinformatics analysis.