Norgen’s Total Nucleic Acid Preservation Tubes are designed for ambient preservation and transport of total nucleic acids (DNA and RNA) from samples collected using a swab, including nasal, buccal, saliva, fecal, skin, surfaces, etc. The Total Nucleic Acid Preservation Tubes contain Norgen’s Total Nucleic Acid Preservative in a liquid format. The user simply collects the specimen using a sterile nylon flocked swab (not provided), and then transfers the swab into the Total Nucleic Acid Preservative. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Total Nucleic Acid Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Preservative allow DNA samples to be stored at room temperature for over 4 months and RNA samples to be stored at room temperature for over 2 months.
Swab samples collected and preserved using Norgen’s Total Nucleic Acid Preservation Tubes are compatible with most commercially available DNA and RNA isolation methods. Samples stored in the tubes have been used successfully with most of Norgen Biotek’s DNA and RNA isolation kits and reagents, including Norgen’s Total RNA Purification Kit (Cat# 17200) and Norgen’s Saliva DNA Isolation Kit (Cat# RU45400). For purification of total nucleic acids please refer to the supplementary protocol provided with Norgen’s Total RNA Purification Kit (Cat#17200).
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| Kit Specifications | |
| Stability of DNA at Room Temperature | 4 months |
| Stability of RNA at Room Temperature | 2 months |
Storage Conditions and Product Stability
The Total Nucleic Acid Preservation Tubes can be stored at room temperature for up 1 year without any reduction in kit performance. The collect before date is indicated on the tube label and the kit box label.
| Kit Components | Cat. 69200 |
|---|---|
| Total Nucleic Acid Preservation Tubes | 50 |
| Product Insert | 1 |
Listeria monocytogenes have emerged as significant foodborne pathogens that pose a serious public health problem. As the causative agent of Listeriosis, L. monocytogenes has the highest rate of fatality rate among all foodborne pathogens. L. monocytogenes is a facultatively intracellular, Gram-positive bacterium that could survive high and low temperatures, low pH. It is a rare causative agent of mastitis in cow. However, due to its ability to resist various food processing technologies as well as to grow at low temperature, L. monocytogenes is know to be associated with both raw and pasteurized milk, as well as dairy products such as cheese. As little as 1000 organisms may cause the disease with pregnant, new-born, and immunocompromised individuals the most susceptible.
Listeria monocytogenes TaqMan PCR Kit, 100 reactions
Listeria monocytogenes TaqMan PCR Probe/Primer Set and Controls, 100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM30450 (100 preps) | Cat. TM30410 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Listeria monocytogenes Primer & Probe Mix | 280 μL | 280 μL |
| Listeria monocytogenes Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
DDL5 Low Speed 5000RPM Swing out rotor 4x800ml Refrigerated Centrifuge Adapters for 5/7/10/15/50ml tubes.
DDL5 Refrigerated Centrifuge Application:
DDL5 Refrigerated centrifuge widely used in the field of blood bank station, pharmaceutical factory and laboratory, can hold 500ml triple, 400ml triple and 250ml triple blood bags.
DDL5 Refrigerated Centrifuge Features:
1. Brushless frequency motor which in great torque, free maintenance, no powder pollution, quick in speed up and down.
2. Digital display which indicates the program, speed, time, RCF and temperature.
3. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration/deceleration for your choice.
4. There are 6 kinds of rotors for your choice.
5. Electric lid lock, compact design, super speed and imbalance protection.
6. The centrifuge body is made of high-quality steel, safe and reliable.
DDL5 Refrigerated Centrifuge Technical Parameters:
| Max Speed:5000r/min | Max RCF:4730xg |
| Max Volume；4x800ml | Power supply:AC220V,50HZ,7A |
| Timer:1~9h/59min | Noise:≤65dBA |
| Dimension:720x540x830mm | Net Weight: 170KG |
| Speed Accuracy:±20r/min | Package (W*D*H):850×650×1280 |
| Temperature range: | -20℃ ~ 40℃ |
DDL5 Refrigerated Centrifuge Matched Rotors:
| Order No. | Rotor type | Max.Speed (rpm) | Max.Volume (ml) | Max.RCF (xg) |
| No30671 | Swing out rotor | 4,000 | 4x800ml(round cup) | 3,450 |
| No30679 | Swing out rotor | 4,000 | 4x500ml(square cup) | 3,450 |
| No30696 | Swing out rotor | 4,000 | 4x500ml(round cup) | 3,530 |
| No31377 | Swing out rotor | 5,000 | 4x50ml | 4,730 |
| 4x100ml | 4,730 | |||
| No30592 | Swing out rotor | 4,000 | 4x30x7ml | 3,140 |
| 4x40x7ml | 3,270 | |||
| 4x18x10ml | 3,140 | |||
| No31491 | Mircoplater rotor | 4,000 | 2x4x96well | 2,910 |
| No31494 | Microplater rotor | 4,000 | 4x4x96well | 2,940 |
| No30627 | Angle rotor | 5,000 | 30x15ml | 3,830 |
| No30641 | Angle rotor | 5,000 | 12x50ml | 3,860 |
| No30642 | Angle rotor | 4,000 | 24x50ml | 2,970 |
| No30614 | Angle rotor | 5,000 | 6x100ml | 3,130 |
| No30643 | Angle rotor | 4,000 | 12x100ml | 2,970 |
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