Listeria monocytogenes has emerged as a significant foodborne pathogen that poses a serious public health problem. As the causative agent of Listeriosis, L. monocytogenes has the highest rate of fatality among all foodborne pathogens. L. monocytogenes is a facultatively intracellular, Gram-positive bacterium. Due to its ability to survive high and low temperatures as well as low pH, it could resist various food processing technologies, as well as grow at food storage temperatures. L. monocytogenes is known to be associated with raw meat, unpasteurized milk and dairy products, vegetables, and seafood. As little as 1000 organisms may cause the disease with pregnant, new-born, and immunocompromised individuals being the most susceptible.
Norgen’s Listeria monocytogenes Quantified Bacterial DNA Standard is prepared from cultured bacteria using Norgen’s sample preparation technology. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as a positive control or PCR quantification standard for Listeria monocytogenes.
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| Volume Provided | 250 μL |
| DNA Quantity | 2 x 104 copies per μL |
Storage Conditions
Upon receipt, store Norgen’s Listeria monocytogenes Quantified Bacterial DNA Standard at -20oC or lower. Avoid multiple freeze-thaw cycle. If needed, prepare smaller working aliquots and store at -20oC or lower.
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
Specifications
| Features | Specifications |
| Main Functions | Extract viral RNA/DNA from non-cell/low cell content biological samples |
| Applications | RT-PCR，PCR，NGS |
| Products | Viral total nucleic acid |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Cell-free body fluid such as plasma, serum, soaking solution and tissue homogenate supernatant |
| Sample amount | 200μl |
| Yield | 2-10μg |
| Elution volume | ≥30μl |
| Time per run | ≤30 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA / RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 Mm Tris, pH 8.0).
Advantages
Kit Contents
| Contents | IVD4173 |
| Purification Times | 100 Preps |
| HiPure Viral Mini Column | 100 |
| 2ml Collection Tubes | 200 |
| PK/Carrier RNA | 50 mg |
| Protease Dissolve Buffer | 5 ml |
| Buffer AL | 30 ml |
| Buffer MW1* | 44 ml |
| Buffer MW2* | 50 ml |
| RNase Free Water | 15 ml |
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
Experiment Data
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
