Introduction

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

Details

Specifications

Features	Specifications
Main Functions	Extract viral RNA/DNA from non-cell/low cell content biological samples
Applications	RT-PCR，PCR，NGS
Products	Viral total nucleic acid
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Cell-free body fluid such as plasma, serum, soaking solution and tissue homogenate supernatant
Sample amount	200μl
Yield	2-10μg
Elution volume	≥30μl
Time per run	≤30 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA / RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 Mm Tris, pH 8.0).

Advantages

• Fast – several samples can be extracted in 20 minutes by column method
• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Safe – no phenol chloroform extraction required
• Sensitive – DNA/RNA can be recovered at the level of PG
• High yield – carrier RNA contained in the product maximize the recovery of trace nucleic acid

Kit Contents

Contents	IVD4173
Purification Times	100 Preps
HiPure Viral Mini Column	100
2ml Collection Tubes	200
PK/Carrier RNA	50 mg
Protease Dissolve Buffer	5 ml
Buffer AL	30 ml
Buffer MW1*	44 ml
Buffer MW2*	50 ml
RNase Free Water	15 ml

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.

Experiment Data

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

For scaling up and fully automating magnetic bead-based nucleic acid extraction

With the Flex Nucleic Acid Extraction Workstation, you can automate your NAE workflow at the scale you need to increase efficiency, reduce errors, and save hands-on time. Flex enables you to automate your nucleic acid isolation and purification workflows using any leading magnetic bead-based system on the market.

Optional add-ons can be purchased at a 10% discount when ordered with the Flex Nucleic Acid Extraction Workstation*

For scaling up and fully automating magnetic bead-based nucleic acid extraction

With the Flex Nucleic Acid Extraction Workstation, you can automate your NAE workflow at the scale you need to increase efficiency, reduce errors, and save hands-on time. Flex enables you to automate your nucleic acid isolation and purification workflows using any leading magnetic bead-based system on the market.

Optional add-ons can be purchased at a 10% discount when ordered with the Flex Nucleic Acid Extraction Workstation*

Propargyl-PEG13-acid consists of an alkyne group and a carboxylic acid. The carboxylic acid reacts with amine groups to form amide bonds in the presence of EDC or HATU as an activator. The alkyne group is commonly used in copper catalyzed azide-alkyne Click Chemistry reactions. The hydrophilic PEG units enhance the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG13-acid consists of an alkyne group and a carboxylic acid. The carboxylic acid reacts with amine groups to form amide bonds in the presence of EDC or HATU as an activator. The alkyne group is commonly used in copper catalyzed azide-alkyne Click Chemistry reactions. The hydrophilic PEG units enhance the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.