Sulfo DBCO-PEG3-NHS ester is a water soluble, click chemistry reagent which can undergo copper free click chemistry Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sulfo DBCO-PEG3-NHS ester is a water soluble, click chemistry reagent which can undergo copper free click chemistry Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Usages:
For determination of total bacterial count.
Principle:
Tryptone provide carbon and nitrogen; yeast extract powder provides B vitamins; glucose to provide energy, agar is the solidifying agent.
Formulation（per liter）:
Tryptone 5.0g
Yeast extract powder 2.5g
Glucose 1.0g
Agar 15.0g
Final pH 7.0 ± 0.2
How to use：
1.Suspend 23.5g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min.
2.Diluted and treated samples.
Quality control：
| Item | The name and number of strain | Growth | Colony |
| 1 | E.Coli ATCC8739 | Good | white |
| 2 | Staphylococcus aureus ATCC6538 | Good | yellow |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle
250ga
K-PHOS
SKU: 700004326
100 assays (manual) / 400 assays (auto-analyser)
| Content: | 100 assays (manual) / 400 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Phosphate |
| Assay Format: | Spectrophotometer, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 360 |
| Signal Response: | Increase |
| Linear Range: | 0.1 to 10 μg of phosphate per assay |
| Limit of Detection: | 0.16 mg/L |
| Reaction Time (min): | ~ 20 min |
| Application examples: | Processed foods and drinks, bakery products, dairy products, waste water samples, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, etc.). |
The Phosphate Assay Kit is a rapid, simple, reliable and accurate method for the specific measurement and analysis of phosphate in water, foodstuffs, beverages and other materials. The phosphate detection reaction employs the defined chemical substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). In the presence of phosphate, the substrate is converted enzymatically by purine nucleoside phosphorylase (PNPase) to the products ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine. An application note describes the dephosphorylation reaction of phytic acid using phytase and alkaline phosphatase (sold separately), and subsequent phosphate detection using the reagents provided in K-PHOS.
This method is suitable for analysis using spectrophotometer and auto-analyser.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.
Need other products? See our complete list of assay kits.
Advantages
The Phosphate Assay Kit is a rapid, simple, reliable and accurate method for the specific measurement and analysis of phosphate in water, foodstuffs, beverages and other materials. The phosphate detection reaction employs the defined chemical substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). In the presence of phosphate, the substrate is converted enzymatically by purine nucleoside phosphorylase (PNPase) to the products ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine. An application note describes the dephosphorylation reaction of phytic acid using phytase and alkaline phosphatase (sold separately), and subsequent phosphate detection using the reagents provided in K-PHOS.