The Acetylcholinesterase Assay Kit provides a convenient method for the detecting AChE activity and screening for inhibitors.
The kit uses DTNB to quantify the thiolcholine produced from the hydrolysis of acetylthiolcholine by AChE.
Detail
The Acetylcholinesterase Assay Kit provides a convenient method for the detecting AChE activity and screening for inhibitors.
The kit uses DTNB to quantify the thiolcholine produced from the hydrolysis of acetylthiolcholine by AChE.
AchE Inhibitor Screening Kit (Colorimetric) provides a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of AchE inhibitors.
ACETYLCHOLINESTERASE (EC 3.1.1.7, AChE), also known as RBC cholinesterase, is found primarily in the blood and neural synapses. AChE catalyzes the hydrolysis of the neurotransmitter acetylcholine into choline and acetic acid, a reaction necessary to allow a cholinergic neuron to return to its resting state after activation. Inhibition of the enzyme leads to acetylcholine accumulation, hyperstimulation of nicotinic and muscarinic receptors, and disrupted neurotransmission. AChE inhibition is an important target for the management of Alzheimer’s disease. In addition to Alzheimer’s disease, AChE inhibitors have been useful in the diagnosis or treatment of diseases such as glaucoma, myasthenia gravis, bladder distention, and more.
The Attogene Acetylcholinesterase Inhibitor Assay is based on an improved Ellman method, in which thiocholine produced by the action of acetylcholinesterase forms a yellow color with 5,5′-dithiobis(2-nitrobenzoic acid). The intensity of the product color, measured at 412nm, is proportionate to the enzyme activity in the sample.
Other Products
[CD4000] SMOChem™ dTTP Solution – Sodium Salt (100 mM), 25ml
Product Info
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Product Info
Description
Ultrapure dTTP supplied as sodium salt in purified water (pH 8.5).
Features
Ideal for PCR amplification and cDNA synthesis
Nuclease and ribonuclease free
Applications
PCR
RT-PCR
Storage
-20°C for 36 months
Document
Ultrapure dTTP supplied as sodium salt in purified water (pH 8.5).
Propargyl-PEG6-Ms is a PEG reagent that enables Click Chemistry reactions with azide-bearing compounds or biomolecules; copper is needed as a catalyst. The PEG spacer increases the water solubility of the reagent in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG6-Ms is a PEG reagent that enables Click Chemistry reactions with azide-bearing compounds or biomolecules; copper is needed as a catalyst. The PEG spacer increases the water solubility of the reagent in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
M-SAN HQ – Peak performance where it matters most at physiological conditions
Medium-Salt Active Nuclease High Quality (M-SAN HQ) is a Bioprocessing Grade nuclease developed for removal of both single and double-stranded DNA and RNA at the physiological salt conditions most often used in bioprocessing and biomanufacturing workflows. M-SAN HQ allows you to directly replace Benzonase without changing your workflow.
This novel, nonspecific endonuclease is active over a broad pH range and displays optimum activity at salt concentrations between 125 – 250 mM. Due to its excellent performance at physiological conditions, M-SAN HQ can be used directly in the cell medium or the harvested supernatant without buffer adjustments. This makes M-SAN HQ ideal for the manufacturing of fragile vectors such as lentiviruses and retroviruses.
M-SAN HQ can be directly used in medium without buffer adjustments The high activity of M-SAN HQ at standard cell medium conditions leads to improved DNA clearance compared to other commonly used nucleases. In the data (see Figure 6), an over 2-fold reduction in residual DNA was achieved.
Key Benefits
Compatibility: Ideal for working with both fragile and robust viral vectors and proteins in a variety of cell media
Optimum activity: Optimization for cell media salinity allows both shorter DNA fragments and reduced incubation times.
Cost-Effectiveness: Reduced need for additional reagents and steps can lower production costs
Quality: Maintains the integrity of sensitive or labile biological molecules, ensuring a higher quality end product
Flexibility: Can be used directly in a variety of media without the need for customization
Key Features
High purity (≥ 99%)
No protease detected
Endotoxin-tested
Animal origin-free production
Supplied with extended product documentation
Adapted to use in medium without salinity adjustments
Optimal activity at physiological salinity and pH makes it ideal for DNA removal from mammalian cell media. (see Figures)
The high activity of M-SAN HQ at the physiological salinity and pH found in standard cell medium conditions leads to improved DNA clearance compared to commonly used nucleases.
Simple to Optimize in Cell Media
M-SAN HQ is uniquely formulated to excel in physiological pH conditions, offering high performance at the commonly used cell media pH of 7.4.
While other nucleases often require more alkaline environments, M-SAN HQ stands out for its adaptability and effectiveness in cell media. It’s the nuclease that truly aligns with your bioprocessing needs. (See Figure section)
M-SAN HQ ELISA Kit
The M-SAN HQ ELISA Kit confirms the removal of M-SAN High Quality in bioprocessing and biomanufacturing applications with high accuracy:
Medium-Salt Active Nuclease High Quality (M-SAN HQ) is a Bioprocessing Grade nuclease developed for removal of both single and double-stranded DNA and RNA at the physiological salt conditions most often used in bioprocessing and biomanufacturing workflows. M-SAN HQ allows you to directly replace Benzonase without changing your workflow.
