The Aflatoxin M1 plate kit is a competitive enzyme-labeled immunoassay. The Aflatoxin M1 sample extract and calibrators are pipetted into the test wells followed by the Aflatoxin M1 antibody into the test wells to initiate the reaction. During the 30 minutes incubation period, Aflatoxin M1 from the sample and Aflatoxin M1 antigen compete for binding to the Aflatoxin M1 antibody. The Aflatoxin M1 antibody is captured on the walls of the test well. Following this 30-minute incubation, the contents of the wells are removed and the wells are washed to remove any unbound Aflatoxin M1 and free Aflatoxin M1 antibody. After wash, 1X HRP-conjugated Antibody#2 is added for 30 minutes incubation. The wells are washed afterwards, and a clear substrate is then added to the wells and any bound enzyme conjugate causes the conversion to a blue color. Following a 15-minute incubation, the reaction is stopped and the amount of color in each well is read. The color of the unknown samples is compared to the color of the calibrators and the Aflatoxin M1 concentration of the samples is derived.
This product is specially designed for stool RNA extraction. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.
Details
Principle
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
Kit Contents
Contents
R662601
R662602
R662603
Purification Times
48 Preps
96 Preps
5 x 96 Preps
MagPure RNA Particles
1.7 ml
4.0 ml
18 ml
DNase I
600 μl
2 x 600 μl
10 x 600 μl
DNase Buffer
30 ml
40 ml
200 ml
Buffer SPL
30 ml
60 ml
270 ml
Buffer PHC
30 ml
60 ml
270 ml
Buffer MCB*
9 ml
15 ml
75 ml
Buffer ALB3*
10 ml
20 ml
100 ml
Buffer GW1*
44 ml
66 ml
2 x 220 ml
Buffer RW2*
20 ml
50 ml
2 x 100 ml
RNase Free Water
10 ml
30 ml
120 ml
2ml Bead Tubes
48
96
5 x 96
Storage and Stability
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
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This product is specially designed for stool RNA extraction. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.
Propargyl-PEG5-CH2CO2tBu enables the formation of triazole linkages with azide compounds or biomolecules in copper catalyzed Click Chemistry reactions. The t-butyl group can be hydrolyzed under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG5-CH2CO2tBu enables the formation of triazole linkages with azide compounds or biomolecules in copper catalyzed Click Chemistry reactions. The t-butyl group can be hydrolyzed under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Floor Type High Speed & Low Speed Universal Refrigerated Centrifuge
Product Info
Document
Product Info
GL20 Technical Parameter：
Max. Speed
21000rpm
Max. RCF
30910×g
Max. Capacity
4×800ml
Time Range
1~9h59min
RPM/RCF Convert
Yes
Noise (dB)
≤ 60
Temperature
-20℃~40℃
Acc/Dec
10 Kinds
Speed Accuracy
±20r/min
Temperature Accuracy
±1℃
Voltage(V/Hz)
AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)
680×540×830mm
Net Weight(Kg)
170KG
Certificates
CE,ISO & Calibration report are available
Matched Rotors for GL20
Order No
Rotor
Rotor Max Speed(r/min)
Volume(ml)
RCF(×g)
G20-1
Swing Rotor
4000
4×800ml
3450
G20-2
Microplate rotor
4000
4×4×96 well
2940
G20-3
Microplate rotor
4000
2×4×96 well
3210
G20-4
Microplate rotor
4000
2×3×48 well
2300
G20-5
Swing rotor
4000
4×40×7ml vacuum tube
3270
G20-6
4000
4×40×5ml vacuum tube
2890
G20-7
4000
4×28×10ml vacuum tube
3130
G20-8
Swing Rotor
4000
Fat bottle
3830
G20-9
Fixed Rotor
16000
4×8PCR
15760
G20-10
Fixed Rotor
15000
6×8PCR
21420
G20-11
Fixed Rotor
16000
8×8PCR
17480
G20-12
Fixed Rotor
15000
12×8PCR
22930
G20-13
Fixed Rotor
15000
40×0.5ml
22920
G20-14
Fixed Rotor
21000
12×1.5ml
30910
G20-15
Fixed Rotor
16000
24×1.5ml
23440
G20-16
Fixed Rotor
14000
30×1.5ml
20800
G20-17
Fixed Rotor
13000
48×1.5ml
17930
G20-18
Fixed Rotor
16000
16×5ml
22020
G20-19
Fixed Rotor
16000
6×10ml
21500
G20-20
Fixed Rotor
15000
12×10ml
22680
G20-21
Fixed Rotor
13000
16×10ml
19490
G20-22
Fixed Rotor
13000
8×15ml
17790
G20-23
Fixed Rotor
11000
12×15ml
14330
G20-24
Fixed Rotor
5000
24×15ml
3500
G20-25
Fixed Rotor
5000
30×15ml
3830
G20-26
Fixed Rotor
14000
6×30ml
19060
G20-27
Fixed Rotor
13000
6×50ml(conical)
18840
G20-28
Fixed Rotor
13000
6×50ml(round)
18730
G20-29
Fixed Rotor
5000
12×50ml
3860
G20-30
Fixed Rotor
4000
24×50ml
2970
G20-31
Fixed Rotor
13000
4×85ml
18940
G20-32
Fixed Rotor
12000
4×100ml
14850
G20-33
Fixed Rotor
10000
6×100ml
11380
G20-34
Fixed Rotor
4000
12×100ml
2970
G20-35
Fixed Rotor
12000
24 capillaries
15800
G20-36
Swing Rotor
15000
4×5ml
19920
G20-37
Vertical rotor
16000
16×5ml
16450
G20-38
Vertical rotor
14000
8×30ml
19750
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Features
1. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.
2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control
3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.
4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.
