Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
African Swine Fever Virus (ASFV) is a widespread disease which infects members of the pig family(Suidae). Anumberoftick species are believed to be the vector for the disease,as well as being transmitted by raw pork and pig excrement [1]. After firstly being identified in Kenya in 1921, ASFV became endemic in sub-Saharan Africa, with regular outbreaks being reported across Europe, Asia and South America throughout the century [2]. More recently the virus was introduced in Georgia and spread throughout the region, as well as mass outbreaks occurring in China in 2018 [3]. ASFVistheonlymemberoftheAsfaridaefamily.ItisalargeenvelopeddoublestrandedDNA virus of icosahedral morphology with an average diameter of 200nm and isolates contain genomes between 170-190Kbp encoding for up to 167 open reading frames [2]. The morphology of ASFV consist of several concentric domains. An inner core contains the nucleoid coated with a thick protein layered core shell, which is surrounded by an inner lipid envelope , all of which is encompassed by the capsid [2]. ASFV begins its replication cycle in the nucleus of infected cells before moving to the cytoplasm where the majority of the replication takes place [2]. Gene transcription is highly regulated, with distinct classes of mRNA identified to accumulate at early, intermediate and late transcripts of the virus [2]. The disease induces acute haemorrhagic disease within its hosts, causing high fevers and skin haemorrhages, with death often occurring within ten days of clinical symptoms appearing [4].
References: 1: The Centre for Food Security and Public Health (2015), African Swine Fever. 2: Galindo, I. and Alonso, C., 2017. African swine fever virus: a review. Viruses, 9(5), p.103. 3: Zhou, X., Li, N., Luo, Y., Liu, Y., Miao, F., Chen, T., Zhang, S., Cao, P., Li, X., Tian, K. and Qiu, H.J., 2018. Emergence of African swine fever in China, 2018. Transboundary and emerging diseases, 65(6), pp.1482-1484. 4: Gallardo, C., Ademun, A.R., Nieto, R., Nantima, N., Arias, M., Martín, E., Pelayo, V. and Bishop, R.P., 2011. Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007. African Journal of biotechnology, 10(17), pp.3488-3497.
Other Products
DBCO-PEG5-NHS ester
Product Info
Document
Product Info
DBCO-PEG5-NHS Ester is a DBCO-containing PEG linker containing NHS ester that can react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. PEG5 spacer increases the water solubility of compounds in aqueous media. DBCO is commonly used for copper-free Click Chemistry reaction. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-PEG5-NHS Ester is a DBCO-containing PEG linker containing NHS ester that can react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. PEG5 spacer increases the water solubility of compounds in aqueous media. DBCO is commonly used for copper-free Click Chemistry reaction. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Blank lateral flow strips are the perfect companion during the development and feasibility testing of a lateral flow device. IN THE BEGINNING, the capture and test line reagents can be spotted onto the nitrocellulose portion of the blank strips at different concentrations, volumes and buffer conditions. This enables the user to determine the optimal conditions for subsequent spraying and enables the user to verify the test and controls will respond according to expectations before scaling up the process.
These blank strips are compatible with all of our colloidal gold products AND:
Lateral flow cassettes: C02022
Streptavidin CdSe/ZnS Quantum Dots For Lateral Flow: AU2043
Streptavidin Europium Chelate Microspheres For Lateral Flow: AU2050
Streptavidin Colloidal Gold For Lateral Flow: AU2017
Twenty 4.5X60mm strips of each combination of nitrocellulose (4X), wicking material (2X), and sample pad (3X)
A total of 480 strips are provided
100 cassettes
125mL of buffer
Chlamydia trachomatis, the causative agent of Chlamydia, is a Gram negative bacteria. Transmission of the bacteria occurs via contact with infected bodily fluids which then infect mucosal membranes. It can be transmitted from mother to child during pregnancy and infect the eyes causing conjunctivitis. The genital infection causes urethritis, epididymitis and prostatitis in males and Pelvic Inflammatory Disease (PID) in females with an increased risk of contracting HIV. The infection can be treated with a course of antibiotics. Sexually transmitted infections in females are most often asymptomatic, but can be noticeable in chronic pain of the pelvic region, vaginal bleeding and painful urination. Infection of the ovaries, fallopian tubes or uterus causes Pelvic Inflammatory Disease (PID) which can lead to difficulties in conceiving, increased risk of ectopic pregnancy or infertility. Infections in males are more likely to be symptomatic, causing painful urination, discharge from the penis and swollen testicles and may eventually cause infertility if left untreated.
Chlamydia TaqMan PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
Chlamydia TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component
Cat. TM30950 (100 preps)
Cat. TM30910 (100 preps)
MDx TaqMan 2X PCR Master Mix
2 x 700 μL
–
Chlamydia Primer & Probe Mix
280 μL
280 μL
Chlamydia Positive Control
150 μL
150 μL
Nuclease-Free Water (Negative Control)
1.25 mL
1.25 mL
Product Insert
1
1
Overview
Detection kits for Chlamydia
Available in TaqMan format for analysis
Chlamydia trachomatis, the causative agent of Chlamydia, is a Gram negative bacteria. Transmission of the bacteria occurs via contact with infected bodily fluids which then infect mucosal membranes. It can be transmitted from mother to child during pregnancy and infect the eyes causing conjunctivitis. The genital infection causes urethritis, epididymitis and prostatitis in males and Pelvic Inflammatory Disease (PID) in females with an increased risk of contracting HIV. The infection can be treated with a course of antibiotics. Sexually transmitted infections in females are most often asymptomatic, but can be noticeable in chronic pain of the pelvic region, vaginal bleeding and painful urination. Infection of the ovaries, fallopian tubes or uterus causes Pelvic Inflammatory Disease (PID) which can lead to difficulties in conceiving, increased risk of ectopic pregnancy or infertility. Infections in males are more likely to be symptomatic, causing painful urination, discharge from the penis and swollen testicles and may eventually cause infertility if left untreated.
Chlamydia TaqMan PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
Chlamydia TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
