The Opentrons Deep Well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold flat bottom plates and other flat labware at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
Detail
The Opentrons Deep Well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold flat bottom plates and other flat labware at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
Other Products
D5001 MagPure Gel Pure DNA Kit
Product Info
Document
Product Info
Introduction
A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.
Details
Specifications
Features
Specifications
Main Functions
Recover DNA fragments >100bp from agarose gel(<0.3g), purification of DNA from PCR, enzymatic reaction solution or crude gDNA
The Kit method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments (>100bp) and larger to paramagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure.
Advantages
High recovery efficiency – up to 80% DNA recovery
General – recover DNA from gel or enzyme-driven reaction solutions such as PCR
Kit Contents
Contents
D500101
D500102
D500103
Purification Times
50 Preps
500 Preps
5000 Preps
Buffer GDP
30 ml
250 ml
3 x 900 ml
MagPure Particles
1.6 ml
15 ml
3 x 50 ml
Buffer DW1
20 ml
180 ml
2 x 800 ml
Elution Buffer(10mmTris, pH8.5)
10 ml
60 ml
500 ml
Storage and Stability
MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
Document
A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.
This kit is suitable for high-throughput extraction of total RNA from paraffin embedded tissue samples, including miRNA. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 120 minutes. The obtained RNA can be directly used in RT-PCR. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 holeo scillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from FFPE tissue
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Products
RNA, miRNA
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Sample type
FFPE slice, FFPE embedded tissue
Sample amount
≤6 slices
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
Fast – several samples can be extracted in 120 minutes by column method
Safe – no phenol chloroform extraction required
Kit Contents
Contents
IVD3022
Purification Times
200 Preps
MagBind Particles
4.5 ml
Proteinase K
100 mg
Protease Dissolve Buffer
6 ml
DNase I
4 x 600 µl
DNase Buffer
30 ml
Buffer DPS
200 ml
Buffer FRL
40 ml
Buffer AL
40 ml
Buffer MW1*
110 ml
Buffer MW2*
2 x 50 ml
Nuclease Free Water
30 ml
Storage and Stability
Proteinase K and MagBind Particles should be stored at 2–8°C upon arrival. DNase I should bestored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K and MagBind Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.
Document
This kit is suitable for high-throughput extraction of total RNA from paraffin embedded tissue samples, including miRNA. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 120 minutes. The obtained RNA can be directly used in RT-PCR. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 holeo scillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.
m-PEG12-DBCO is a long chain click chemistry PEG reagents. The DBCO group can react with azides via a copper-free Click Chemistry reaction to form a stable triazole. This reagent can be applied to situations when copper is a concern for DBCO-activated PEGylation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
m-PEG12-DBCO is a long chain click chemistry PEG reagents. The DBCO group can react with azides via a copper-free Click Chemistry reaction to form a stable triazole. This reagent can be applied to situations when copper is a concern for DBCO-activated PEGylation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.