The Opentrons Deep Well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold flat bottom plates and other flat labware at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
The Opentrons Deep Well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold flat bottom plates and other flat labware at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
12 x 8 strips (96 tests)
Cysticercosis is caused by larval cysts of the tapeworm Taenia solium and is a major cause of adult onset seizures in most developing countries. Diagnosis is based on imaging findings, but in many cases it is not conclusive for the diagnosis. Serological assays has an important place on the final diagnosis of the disease. The ELISA kit hs shown appropriate performances for the serological screening of cysticercosis.
Name of Product
Taenia solium – IgG ELISA
Catalog Number
AF 9700
Short Info
Cysticercosis is caused by larval cysts of the tapeworm Taenia solium and is a major cause of adult onset seizures in most developing countries. Diagnosis is based on imaging findings, but in many cases it is not conclusive for the diagnosis. Serological assays has an important place on the final diagnosis of the disease. The ELISA kit hs shown appropriate performances for the serological screening of cysticercosis.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
98% sensitivity, 98% specificity
Test Principle
The kit provides all the material needed to perform 96 enzyme-linked immunosorbent assays (ELISA) on breakable microtitration wells sensitized with Taenia solium cyst soluble antigens. Specific antibodies in the sample will bind to these antigens and washing will remove unspecific antibodies. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate. A second washing step will remove unbound conjugate. Revealing bound antibodies is made by the addition of pNPP substrate which turns yellow in the presence of alkaline phosphatase. Color intensity is proportional to the amount of Taenia solium specific antibodies in the sample. Potassium phosphate is added to stop the reaction. Absorbance at 405 nm is read using an ELISA microplate reader. The test can be performed with automatic systems, but this must be validated by the user.
Cysticercosis is caused by larval cysts of the tapeworm Taenia solium and is a major cause of adult onset seizures in most developing countries. Diagnosis is based on imaging findings, but in many cases it is not conclusive for the diagnosis. Serological assays has an important place on the final diagnosis of the disease. The ELISA kit hs shown appropriate performances for the serological screening of cysticercosis.
Different-sized amplicons from 3 ng of a DNA plasmid were amplified. The use of Taq DNA polymerase resulted in clean and high yield of products, as analysed after PCR on a 0.8% agarose gel.
A fragment (64 bp) of the human blood-coagulation factor IIa (F2) was amplified from 20 ng, 2 ng, 200 pg and 20 pg of a human genomic DNA extract. The same experiment was performed in parallel using the Taq DNA polymerase mix from another well-established and known supplier. PCR products were subsequently analysed on a 2.5% agarose gel.
Taq 2x PCR Master Mix – ready to use mix simplifies your PCR setup. Only primers and template need to be added as the mix contains all copmponents for a successful and reliable PCR. This ensures reproducible results, significantly reduces set-up times and the risk of pipetting errors. The robustness of PCR performance allows the application of this mix in a wide range of PCR amplifications.
