Aluminum Block – 96-well PCR Plate

Introduction

Place of Origin: Guangdong, China

Warranty: 2 years

Customized support: OEM

Brand Name: HKM

Model Number: CRM011

Reagent grade: Biochemical Reagent

Form: Powder

Color: Light yellow

Type: Shigella Agar

1000mL

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Eight discrete fragments from 50 bp to 2000 bp
• Higher intensity reference band at 500 bp

The FastRunner DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains eight discrete fragments ranging from 50 bp to 2000 bp with a higher intensity reference band at 500 bp. This Ladder is ideal for quick sizing of PCR products and restriction digests.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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FastRunner DNA Ladder (Cat# 12800) – 100 loads

Ladder Properties:
• Eight discrete bands, ranging from 50 bp to 2000 bp
• Higher intensity band at 500 bp for easy reference

Fragment	Size (bp)	Mass (ng)
1	2000	104
2	1500	88
3	1000	68
4	750	59
5	500	93
6	300	28
7	150	35
8	50	25

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 
Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

DBCO-Sulfo-NHS ester is a water-soluble sulfocated reagent containing DBCO moiety. It can be used for simple and efficient labeling of antibodies, proteins and any other primary amine-bearing macromolecules with DBCO moiety in 100% aqueous buffers. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-Sulfo-NHS ester is a water-soluble sulfocated reagent containing DBCO moiety. It can be used for simple and efficient labeling of antibodies, proteins and any other primary amine-bearing macromolecules with DBCO moiety in 100% aqueous buffers. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.