The Opentrons 96-well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold full, half, and non skirted PCR plates as well as strip tubes at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
The Opentrons 96-well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold full, half, and non skirted PCR plates as well as strip tubes at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
N-(Boc-PEG1)-N-bis(PEG2-propargyl) is a click chemistry branched linker. The propargyl groups can react with azide-bearing molecule via copper catalyzed Click Chemistry . The Boc group can be deprotected under acidic conditions to release amine group. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
N-(Boc-PEG1)-N-bis(PEG2-propargyl) is a click chemistry branched linker. The propargyl groups can react with azide-bearing molecule via copper catalyzed Click Chemistry . The Boc group can be deprotected under acidic conditions to release amine group. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Plasmid isolation from bacterial cultures is one of the most popular techniques in biomedical research and pharmaceutical industries. However, it is common that the isolated plasmid DNA is usually contaminated with varied degrees of host RNA. Plasmid purification is necessary to reduce the impact on downstream applications by removing RNA contamination.
We have developed a simple reagent to completely remove RNA contamination in the isolated plasmid samples using Solid Phase Reversible Immobilization (SPRI) beads. SPRI beads consist of paramagnetic particles coated with carboxyl groups that reversibly bind DNA. Our Plasmid Purification Magnetic Beads (RNA Depletion) combines BioDynami’s proprietary chemistries with the reversible DNA-binding properties of SPRI magnetic beads. The reagent removes RNA and recovers the plasmid in the same step. Moreover, unwanted components such as salts, dNTPs, proteins, enzymes, and other impurities can also be removed simultaneously.
Plasmid can be used for downstream applications such as enzymatic digestion, transformation, transfection and molecular cloning etc. The beads can be an effective and inexpensive reagent for bacterial RNA depletion for routine plasmid purification.
Features
Plasmid isolation from bacterial cultures is one of the most popular techniques in biomedical research and pharmaceutical industries. However, it is common that the isolated plasmid DNA is usually contaminated with varied degrees of host RNA. Plasmid purification is necessary to reduce the impact on downstream applications by removing RNA contamination.
Usages:
For selective isolation of Gram-negative bacteria, especially for Shigella and Salmonella.
Principle:
Yeast extract powder provide nitrogen, vitamins, growth factors; sodium chloride to maintain osmotic equilibrium ferric ammonium citrate of iron salts to produce a black iron sulfide; agar as medium coagulant; phenol red as pH indicator.
Formulation（per liter）:
Xylose 3.50g
L-Lysine 5.00g
Lactose 7.50g
Sucrose 7.50 g
Sodium Chloride 5.00 g
Yeast Extract 3.00g
Sodium Desoxycholate 2.50g
Sodium Thiosulphate 6.80g
Fe-Ammonium Citrate 0.80g
Phenol Red 0.08g
Agar 13.50
Final PH 7.4±0.2
How to use:
1.Suspend 55.2g in 1Lof distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Do not autoclave ,cool to 50℃ and pour into sterile petri dishes.
2.Dilluted and treated samples.
Quality control:
Quality control strains were inoculated ,and cultured at 36 ± 1 ℃ for 24h ,results show as follows:
strain name strain code growth feature
Arizona bacteria CMCC (B) 47001 good black colonies
Salmonella typhimurium CMCC (B) 50115 good black colonies
Streptococcus faecalis CMCC32223 prohibited —
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
Specifications: 250g/bottle
250g
