The Opentrons Flat Bottom Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold flat bottom plates and other flat labware at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2). This version is only compatible for the Opentrons Flex.
Detail
The Opentrons Flat Bottom Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold flat bottom plates and other flat labware at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2). This version is only compatible for the Opentrons Flex.
Other Products
Cellulase Assay Kit (CellG3 Method)
Product Info
Document
Product Info
K-CellG3
180 / 360 assays per kit
Content:
180 / 360 assays per kit
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
endo-Cellulase
Assay Format:
Spectrophotometer, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
400
Signal Response:
Increase
Limit of Detection:
0.05 U/mL
Reproducibility (%):
~ 3%
Total Assay Time:
~ 20 min
Application examples:
Fermentation broths, industrial enzyme preparations and biofuels research.
The CellG3 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-D-cellotrioside (BCNPG3) and 2) thermostable β-glucosidase. The benzylidene blocking group prevents any hydrolytic action by the β-glucosidase on BCNPG3. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 2-chloro-4-nitrophenol is therefore directly related to the hydrolysis of BCNPG3 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
Please note that a new assay kit (K-CellG5) is now available for the measurement of endo-cellulase. The CellG5 reagent contains a cellopentaose core and exhibits vastly improved sensitivity for some cellulases. In addition, the exchange of the benzylidene blocking group in CellG3 for 3-keto-butylidene in CellG5 improves the substrate’s water solubility significantly, allowing for a reduction in the concentration of DMSO required in the assay. As DMSO is known to inhibit certain cellulases, this is another benefit in using CellG5. Megazyme now recommends the use of K-CellG5 for all assays for the measurement of endo-cellulase.
The CellG3 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components;
1) 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-D-cellotrioside (BCNPG3) and 2) thermostable β-glucosidase. The benzylidene blocking group prevents any hydrolytic action by the β-glucosidase on BCNPG3. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 2-chloro-4-nitrophenol is therefore directly related to the hydrolysis of BCNPG3 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
Opentrons OT-2 Tips, 20µL. Optimized for volumes 1µL to 20µL. Clear Tips, Polypropylene. These tips are DNAse/RNAse, pyrogen and protease free – they are sterilized from ebeam irradiation. Not autoclavable.
Compatible with: Opentrons 8-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN1 P10 Electronic Pipettes, Opentrons 8-Channel GEN1 P10 Electronic PipettesOpentrons platform and workstation accuracy and precision performance is not validated with use of non-Opentrons tips.
Document
Opentrons OT-2 Tips, 20µL. Optimized for volumes 1µL to 20µL. Clear Tips, Polypropylene. These tips are DNAse/RNAse, pyrogen and protease free – they are sterilized from ebeam irradiation. Not autoclavable.
Compatible with: Opentrons 8-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN1 P10 Electronic Pipettes, Opentrons 8-Channel GEN1 P10 Electronic PipettesOpentrons platform and workstation accuracy and precision performance is not validated with use of non-Opentrons tips.
【Material】The vial storage box is made of durable cardboard with waterproof coating and cardboard dividers;chemically resistant to alcohols and mild organic
【Temperature Range】These freezer boxes are stable from -196°C to 121°C
【Applications】The freezer box is suitable for iquid nitrogen freezing (vapor phase)
【Capacity】Size: Height of 2 inch, Holds 0.5ml, 1.5ml, 2.0ml tubes
【Excellent Cusomer Service】We are dedicated in providing the best products and services to customers. If you have any problem, please feel free to contact us. We will help you solve the problem as soon as possible.
Document
【Material】The vial storage box is made of durable cardboard with waterproof coating and cardboard dividers;chemically resistant to alcohols and mild organic
【Temperature Range】These freezer boxes are stable from -196°C to 121°C
【Applications】The freezer box is suitable for iquid nitrogen freezing (vapor phase)
【Capacity】Size: Height of 2 inch, Holds 0.5ml, 1.5ml, 2.0ml tubes
【Excellent Cusomer Service】We are dedicated in providing the best products and services to customers. If you have any problem, please feel free to contact us. We will help you solve the problem as soon as possible.