Aluminum Block Set

Description

Attogene Fluorescent Universal Lateral Flow Assay Kits are a convenient ready-to-use kit for quick and cost-effective development of a fluorescent lateral flow dipstick assay for detection of DNA and RNA products.

Fluorescent lateral flow assays can be 2-100 fold more sensitive that gold based lateral flow tests.  Europium dyed polystyrene beads conjugated to streptavidin

Formats (fluorescent broad range UV light excitation range of 300nm to 400nm, 610nm emission) Streptavidin conjugate pad):

• • Detectable using a black light such as a black light UV flashlight or fluorescent lateral flow reader.
• • Detection of nucleic Acid (DNA or RNA) requires the use of a biotin and FAM-labelled primer during amplification. 
• • Test line: anti-FITC/FAM
• • Control Line: Biotin
• • Multiplex detection of nucleic Acid (DNA or RNA) requires the use of a biotin, FAM and Dig labelled primers during amplification.:
• • Test Line #1: anti FITC/FAM, Line #2: anti-Dig, Line #3 Biotin.

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Inquire about custom configurations: sales@attogene.com. 

For example, this product can be configured with alternative/custom streptavidin fluorescent particles.

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Kit Components 

• 50 -4.5mm Fluorescent Lateral Flow Dipsticks
• 10 mL Sample assay running buffer

Features & Benefits

• Can be used for development of a lateral flow assay for detection of a variety of different molecules such as amplified DNA products from PCR, LAMP and RPA reactions.
• No need to stripe capture antibodies
• No expensive equipment required (Black Light)
• Cost-effective way to screen for further downstream lateral flow assay development.

Overview

• Detection kits for the BKV
• Available in TaqMan format for analysis

The BK virus is a member of the polyomavirus family. BK viral infections are typically asymptomatic in healthy individuals, however very mild symptoms may appear including mild respiratory infections and fever. Once an individual has been infected the virus disseminates to the kidneys and the urinary tract where it remains for the lifetime of the individual. Infections with BK virus in immunocompromised or immunosupressed patients are much more severe and may involve renal dysfunction. In fact, in kidney transplant patients the immunosupressive drugs required for the transplant may allow the virus to replicate within the graft, resulting in a disease called BK virus nephropathy (BKVN). It is thought that 1-10% of renal transplant patients progress to BK virus nephropathy (BKVN) and up to 80% of these patients are reported to have lost their grafts. The onset of nephritis can occur as early as several days post-transplant to as late as 5 years. The mode of transmission of the virus is not clear, however it has been suggested that BKV may be transmitted through respiratory fluids or urine, since infected individuals periodically excrete virus in the urine. This virus can be diagnosed by BKV blood & urine testing, in addition to carrying out a biopsy in the kidneys. PCR techniques are now widely used to identify the virus.

BKV TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

BKV TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

Figure 1 / 3

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM36550 (100 preps)	Cat. TM36510 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
BKV Primer & Probe Mix	280 μL	280 μL
BKV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings 150 reactions