Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane TFA Salt is a crosslinker consisting of an amino group with three propargyl groups. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane TFA Salt is a crosslinker consisting of an amino group with three propargyl groups. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Usages:
For cultivation of non-fastidious microorganisms, indole testing.
Principle:
Peptone provide carbon and nitrogen sources, vitamins and growth factors; sodium chloride to maintain osmotic equilibrium.
Formulation(per liter):
Peptone 10g
Sodium chloride 5g
Final pH 7.0 ± 0.2
How to use:
1.Suspend 15g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min.
2.Diluted and treated samples.
Quality control:
| Item | The name and number of strain | Growth | Colony Color |
| 1 | Escherichia coli ATCC25922 | Good | Turn Red |
| 2 | Klebsiella pneumoniae | Good | Color unchange |
500g
DBCO-PEG1-acid is an analog of DBCO-Acid with hydrophilic PEG linker and a DBCO group. The DBCO groups is commonly used for Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG1-acid is an analog of DBCO-Acid with hydrophilic PEG linker and a DBCO group. The DBCO groups is commonly used for Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Description
The DM4100 ExcelBand™ XL 25 kb DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder DM4100 is composed of 14 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring.
Features
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
250 ~ 25,000 bp
Concentration
51.6 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Room temperature for 6 months
4°C for 12 months
-20°C for 36 months
The DM4100 ExcelBand™ XL 25 kb DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder DM4100 is composed of 14 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring.
