Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane TFA Salt

Overview

Pierceable heat sealing foil which has a high solvent resistance. This seal is resealable, suitable for PCR, compound storage and sample shipping.

• Heat sealing offers a 100% effective method of plate sealing, for complete seal integrity, as well as being quick and cost effective
• PierceASeal Foil is compatible with polypropylene and polystyrene plates
• This seal demonstrates good solvent resistance and can be used for low temperature and room temperature compound storage in DMSO and organic solvents
• PierceASeal Foil can be pierced with a pipette tip manually, or by a liquid handling robot
• This seal can be resealed by applying another PierceASeal Foil seal straight on top of a previously pierced seal
• This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
• Also available in multiple roll formats compatible with specified automated heat sealers, such as our Wasp or Chameleon XT
• A green dotted line on the sheet foils clearly indicates the non-sealing surface, for ease of seal orientation and handling

Pierceable heat sealing foil which has a high solvent resistance. This seal is resealable, suitable for PCR, compound storage and sample shipping.

Overview

• Simultaneous concentration, desalting and buffer exchange of total urinary proteins
• No molecular weight cutoff allows for isolation of all sizes of proteins and peptides
• Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

These kits concentrate urine proteins while simultaneously removing salts, urea, and other urine contaminants. There is no molecular weight cut-off and therefore the columns capture total urinary proteins and peptides of all sizes making them ideal for biomarker discovery work, differential expression of proteins in various diseases, or other diagnostic research. The columns are convenient, rapid and easy to use and thus offer significant time savings over classic dialysis protocols. The resulting high-quality protein sample is concentrated and free from the original sample salts, thus preparing the sample conveniently for downstream proteomic applications including SDS-PAGE, 2D Gels, MALDI-TOF, LC/MS, LC/MS/MS, whole protein mass spectrometry, western blotting, protein microarrays, and more.

ProteoSpin™ Urine Protein Concentration Micro Kit

Each spin column is able to concentrate and desalt up to 200 μg of urine proteins. Twelve samples can be processed in 20 minutes.

ProteoSpin™ Urine Protein Concentration Midi Kit

The ProteoSpin™ Urine Protein Concentration Midi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 1 to 5 mL inputs of urine. Each mini spin column is able to concentrate and desalt up to 3 mg of urine proteins in 30 minutes.

ProteoSpin™ Urine Protein Concentration Maxi Kit

The ProteoSpin™ Urine Protein Concentration Maxi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 2 to 20 mL inputs of urine. Each maxi spin column is able to concentrate and desalt up to 4 mg of urine proteins in 45 minutes.

Details

Supporting Data

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Kit Specifications
Maximum Urine Input Volume	20 mL
Maximum Recovered Protein	4 mg
Minimum Elution Volume	2 mL
Time to Process 4 Samples	45 minutes

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

Component	Cat. 17400 (25 preps)	Cat. 52300 (10 preps)	Cat. 21600 (4 preps)
Wash Solution C	60 mL	60 mL	130 mL
Binding Buffer A	4 mL	4 mL	8 mL
Elution Buffer C	8 mL	30 mL	30 mL
Protein Neutralizer	4 mL	4 mL	4 mL
Micro Spin Columns	25	–	–
Midi Spin Columns (assembled with collection tubes)	–	10	–
Maxi Spin Columns (assembled with collection tubes)	–	–	4
Collection Tubes	25	–	–
Elution Tubes (1.7 mL)	25	–	–
Midi Elution Tubes	–	10	–
Elution Tubes (50 mL)	–	–	4
Product Insert	1	1

Product Description

Amp-future DNA Isothermal Amplification Kit NFO: Freeze-Dried Reagent

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.