Aminooxy-PEG1-propargyl is a click chemistry PEG linker containing a propargyl group and an aminooxy group. The aminooxy group is reactive with an aldehyde or ketone group to form a stable oxime linkage. The propargyl group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.
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Aminooxy-PEG1-propargyl is a click chemistry PEG linker containing a propargyl group and an aminooxy group. The aminooxy group is reactive with an aldehyde or ketone group to form a stable oxime linkage. The propargyl group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.
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ProbeSure™ Master Mix
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Hydrolysis probe genotyping master mix for use with 5’ nuclease assays such as TaqMan™, BHQ®, BHQplus® & Zen™ probes. Accurate, robust performance at competitive prices.
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ProbeSure Master Mix combines class-leading performance with the most competitive prices in the market. ProbeSure Master Mix has provided users with accurate, robust, and consistent performance for many years. It has been carefully optimised to work over a wide range of reaction volumes and sample template qualities.
ProbeSure Master Mix is supplied at 2x concentration for convenience and is supplied with the ROX normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ROX.
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Hydrolysis probe genotyping master mix for use with 5’ nuclease assays such as TaqMan™, BHQ®, BHQplus® & Zen™ probes. Accurate, robust performance at competitive prices.
Cell Culture Media Exosome Purification and RNA Isolation Kits
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Overview
Purification and enrichment of intact cell culture media exosomes for functional studies
Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
Versatile cell culture media input volumes
No phenol extractions, Proteinase K treatment, nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents nor overnight incubation required
Pure exosomes are purified and are free from any other RNA-binding proteins
Purification is based on spin column chromatography that uses Norgen’s proprietary resin
The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Norgen’s Cell Culture Media Exosome Purification and RNA Isolation Kits constitute an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Cell Culture Media Exosome Purification and RNA Isolation Mini Kit
For sample input volumes ranging from 5 mL to 10 mL.
Cell Culture Media Exosome Purification and RNA Isolation Midi Kit
For sample input volumes ranging from 10 mL to 20 mL.
Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit
For sample input volumes ranging from 20 mL to 35 mL.
All sizes, including miRNA and small RNA (<200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields*
Variable depending on specimen
*Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods.
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60ºC if any salt precipitation is observed.
Tonsil, Follicular Lymphoma, Diffuse Large B-Cell Lymphoma
Dilution Range
1:200
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LMO2, also known as LIM-Only transcription factor 2, RBTN2, or TTG2, is an oncoprotein that is expressed in normal germinal center B-cells, as well as bone marrow hematopoietic precursors and endothelial cells. LMO2 plays a role in angiogenesis and hematopoesis, and its expression has been detected in erythroid and myeloid precursors, megakaryocytes, and also in lymphoblastic and acute myeloid leukemias. LMO2 protein expression has been noted in diffuse large B-cell lymphoma, the most common adult non-Hodgkin lymphoma, as well as follicular lymphoma, a neoplasm derived from germinal center B-cells that accounts for a number of cases of non-Hodgkin lymphomas.
