Cell Washer/Serofuge Centrifuge

Features

1. Microprocessor control, less noisily, it is widely used to qualitative analysis of blood serum, plasma and urea in the fields of hospital, laboratory and biochemistry.

2. Brushless motor, free maintenance, no powder pollution, quick in speed up and down.

3. Special program, HLA and SERO separately.

5. Micro computer control system, LED display the RCF，time and speed.

6.. Electric lid lock, compact design, super speed and imbalance protection.

TD4 Technical Parameter：

Max. Speed	4000rpm
Max. RCF	2000
Max. Capacity	12×7
Time Range	0~99min
RPM/RCF   Convert	Yes
Noise (dB)	≤ 55
Temperature	Normal
Acc/Dcc	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	/
Voltage(V/Hz)	AC 220V/110V  50HZ/60HZ
Size (W x   D x Hmm)	485×320×255mm
Net Weight(Kg)	24KG

Matched Rotors for TD4

Order No.	Rotor	Max Speed (rpm)	Max   Volume(ml)	Max. RCF（×g）
NO31501	SERO Rotor	3000	12×7/5ml(10-13*60-100mm)	2000
NO31502	HLA Rotor	4000	12×2/1.5/0.5ml	1000

Rotor Name	Max RCF(×g)	Use
HLA rotor for Lymphocyte washing	2000	Lymphocyte separation incubated cell separation
	1000	Hematoblast extenterate(thrombin disposal)
	1000	Lymphocyte washing
SERO rotor for erythrocyte   washing	500	Blood type determination, the image   of the hematocyte
	1000	The test of intersectant aptness
	1000	Hematocyte washing, the distillation of the serum and   plasma

TD4 cell washer centrifuge it is widely used to qualitative analysis of blood serum, plasma and urea in the fields of hospital, laboratory and biochemistry.

Bis-propargyl-PEG14 is a homobifunctional crosslinker that can participate in Click Chemistry to yield a stable triazole linkage with azide; copper is needed as a catalyst. With PEG chain embedded in the molecule, the hydrophilicity is greatly improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Bis-propargyl-PEG14 is a homobifunctional crosslinker that can participate in Click Chemistry to yield a stable triazole linkage with azide; copper is needed as a catalyst. With PEG chain embedded in the molecule, the hydrophilicity is greatly improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.

Relevant reagents were pre-packed in 96-well plates in accordance with the optimal protocol.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 200μl whole blood
Applications	PCR, southern bolt and virus detection, etc
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
Sample amount	200μl
Elution volume	≥50μl
Time per run	≤60 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• High binding force – suitable for handling DNA rich samples, such as whole blood, buffy coat, concentrated blood, etc.
• Fast – polydisperse magnetic beads, fast magnetic response and short extraction time
• High purity – the obtained DNA can be directly used for second-generation sequencing, PCR based detection, gene bank, etc.
• Automatic – saving time andlabor and safer

Kit Contents

Contents	D631101	D631102	D631103
Purification Times	48	96	480
MagPure Particles	1.2 ml	2.5 ml	11 ml
Proteinase K	24 mg	50 mg	220 mg
Protease Dissolve Buffer	1.8 ml	5 ml	15 ml
Buffer AL	15 ml	30 ml	120 ml
Buffer BD	5 ml	10 ml	50 ml
Buffer GW1	22 ml	53 ml	220 ml
Elution Buffer	15 ml	30 ml	100 ml

Storage and Stability

Proteinase K, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage(up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.

Relevant reagents were pre-packed in 96-well plates in accordance with the optimal protocol.