Aminooxy-PEG3-propargyl has a alkyne group and an aminooxy group. The aminooxy group reacts with aldehydes or ketones to form a stable oxime linkages. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.
Aminooxy-PEG3-propargyl has a alkyne group and an aminooxy group. The aminooxy group reacts with aldehydes or ketones to form a stable oxime linkages. The alkyne group can participate in copper catalyzed azide-alkyne Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.
These kits are able to isolate all sizes of circulating and exosomal RNA, including microRNA, without the use of phenol or chloroform. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating and exosomal RNA from large sample volumes. RNA can be isolated from either fresh or frozen samples using this kit. This kit is suitable for the isolation of RNA from serum or plasma prepared from blood collected only on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications including RT-PCR. The purified plasma/serum free-circulating and exosomal RNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.
Free-circulating plasma and serum RNA can serve as both tumor- and fetal-specific markers for cancer detection and prenatal diagnosis. As well, free-circulating RNAs have the potential to provide biomarkers for other disease states. Free-circulating RNA in plasma or serum are usually present as short fragments of less than 1000nt, and free-circulating miRNA (21nt) can also be found in plasma and serum.
Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry)
Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 5 mL.
Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Mini Slurry)
Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 2 mL.
Plasma/Serum Circulating and Exosomal RNA Purification 96-Well Kit (High Throughput Slurry)
Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification 96-Well Kit (Slurry Format) provides a high throughput method for isolating circulating and exosomal RNA from plasma and serum samples using either a vacuum manifold or centrifugation.
Plasma/Serum Circulating and Exosomal RNA Purification Maxi Kit (Maxi Slurry)
Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from various amounts of plasma/serum ranging from 2 mL to 5 mL.
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| Kit Specifications – 96-well | |
| Minimum Plasma/Serum Input | 0.25 mL |
| Maximum Plasma/Serum Input | 2 mL |
| Size of RNA Purified | All sizes, including microRNA |
| Time to Complete Purification | < 1 hour |
Cat.29500 Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 51000 (50 preps) | Cat. 29500 (96 preps) | Cat. 42800 (50 preps) | Cat. 50900 (25 preps) |
|---|---|---|---|---|
| Slurry C1 | – | – | – | 6 mL |
| Slurry C2 | 12 mL | – | 12 mL | – |
| Slurry C3 | – | 20 mL | – | – |
| Lysis Buffer A | 2 x 130 mL | 4 x 100 mL | 2 x 130 mL | 2 x 130 mL |
| Wash Solution A | 38 mL | 2 x 38 mL | 38 mL | 18 mL |
| Elution Solution A | 6 mL | 20 mL | 6 mL | 6 mL |
| Mini Filter Spin Columns | 50 | – | 50 | 25 |
| 96-Well Filter Plate | – | 1 | – | – |
| Adhesive Tape | – | 1 | – | – |
| Collection Tubes | 50 | – | 50 | 25 |
| 96-Well Collection Plate | – | 1 | – | – |
| Elution Tubes (1.7 mL) | 50 | – | 50 | 25 |
| 96-Well Elution Plate | – | 1 | – | – |
| Product Insert | 1 | 1 | 1 | 1 |
Description
The ExcelTaq™ Blood Direct DNA Polymerase is designed for amplifying targeted DNA directly from whole blood, eliminating the need for a lengthy DNA isolation process. ExcelTaq™ Blood Direct DNA Polymerase is highly tolerant in the presence of PCR interfering/ inhibiting substances in blood, such as IgG, hemoglobin, and lactoferrin. ExcelTaq™ Blood Direct DNA Polymerase is compatible with most anticoagulants, such as citrate, EDTA, and heparin (Fig. 1). The ExcelTaq™ Blood Direct DNA Polymerase includes a pair of Positive Control Primers (CCR5) that are compatible with primate blood samples.
Features
Applications
Storage
-20°C for 24 months
4℃ for 6 months
The ExcelTaq™ Blood Direct DNA Polymerase is designed for amplifying targeted DNA directly from whole blood, eliminating the need for a lengthy DNA isolation process. ExcelTaq™ Blood Direct DNA Polymerase is highly tolerant in the presence of PCR interfering/ inhibiting substances in blood, such as IgG, hemoglobin, and lactoferrin. ExcelTaq™ Blood Direct DNA Polymerase is compatible with most anticoagulants, such as citrate, EDTA, and heparin (Fig. 1). The ExcelTaq™ Blood Direct DNA Polymerase includes a pair of Positive Control Primers (CCR5) that are compatible with primate blood samples.
