Ammonia Assay Kit (Liquid Ready™)

Cell Culture Dish 100mm

Cell Culture Dish provide Excellent surface smoothness gives clear microscopic observation

Specifications: 35mm. 60mm.100mm.150mm

PRODUCT FEATURES

• The product is made of medical grade USP CLASS VI polymer polystyrene
• The product is made under a 100,00- class dust-free manufacturing site
• Two kinds of product line up are providing.

For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.

For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.

Gamma radiation sterilization

• Non-Pyrogenic, DNase/Rnase free.

Cell Culture Dish provide Excellent surface smoothness gives clear microscopic observation

Specifications: 35mm. 60mm.100mm.150mm

Product Details

Application:

DNA Nucleic Acid

Format:

NFO Kit

Kit Components:

Reagents,Buffer,Enzymes

Manufacturer:

Amp-future Bio

Product Name:

DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type)

Reaction Time:

5-20mins

Reaction Volume:

50μL

Sensitivity:

500-1000copies/ml

Shelf Life:

14 Months

Specificity:

High

Storage Conditions:

-20℃

Suitability:

Universal

High Light:

Isothermal DNA Amplification Kit

, 

DNA Amplification Kit NFO

, 

20mins nucleic acid amplification

Payment & Shipping Terms

Minimum Order Quantity

48T

Price

USD$3.8/T

Packaging Details

16T/Bag,48T/Box

Delivery Time

6 working days

Payment Terms

T/T, MoneyGram

Supply Ability

100000T/Month

Product Description

DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type)

【Principle Overview】

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins, the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins; along with the dissociation of the recombinase from the complex, the polymerase also binds to the 3′ end of the primer and begins chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

【Primer design】

It is recommended to use primers with a length of 30-35 bp.Too short primers will affect the amplification speed and detection sensitivity;the 5′ end of the reverse primer is labeled with a modification group (commonly used biotin).

Primers are designed to avoid the formation of secondary structures that affect amplification; the length of the amplicon is recommended to be 150-500bp.

【Colloidal gold probe design】

In the middle of the forward and reverse primer,design a sequence of 46-52nt in length that is complementary to the target fragment; modify an antigen marker (typical FAM) at the 5′ end;mark a dSpacer (tetrahydrofuran, THF) in the middle of the 5′ end and the 3′ end ), as the recognition site of nfo;the 3′ end is labeled with a modification group, such as amine group, phosphate group or C3-Spacer, etc.

【Features】

This kit has the advantages of high sensitivity, strong specificity,and short reaction time (only 15 minutes),and the reaction components are in dry powder state,which is easy to operate and easy to store.

This reagent has low equipment requirement,and the reaction operation can be carried out in metal baths, water baths, etc.,and there is no need to purchase expensive exclusive equipment such as PCR amplifiers.

Composition	Content
A buffer	1.6mL×1Tube
B buffer	150μL×1Tube
Positive control template-II	100μL×1Tube
Positive control probe and primer mix-II	70μL×1Tube
Reagent	48 T
Guide Manual	1 Copy

【Kit storage】

1. Storage conditions: storage temperature ≤ -20℃constant temperature environment, keep away from light and avoid heavy pressure;

2. Product validity period: 14 months;

3. See the outer packaging for the production date.

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins, the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins; along with the dissociation of the recombinase from the complex, the polymerase also binds to the 3′ end of the primer and begins chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

Overview

• Reliable and cost-effective
• Non-invasive, user-friendly, convenient sample collection & preservation in a single tube system
• Preserved exosomes are stable for 2 years at ambient temperature
• Shipping accessories can be purchased separately
• Compatible with Norgen’s Saliva Exosome Purification Kit (Cat. 65300)
• RNA can be isolated from the purified exosomes using Norgen’s Exosome RNA Purification kit (Cat. 58000)

Norgen’s Saliva Exosome Collection and Preservation Kit is an all-in-one solution designed for 1) simple and non-invasive saliva collection; 2) preservation of exosomes in saliva samples at ambient temperature; and 3) isolation of high quality intact exosomes and exosomal RNA within a laboratory setting. The Saliva Exosome Collection, Preservation and Isolation Kit contains 50 Individual Saliva Exosome Collection and Preservation Devices. Each of the 50 individual Saliva Exosome Collection and Preservation Devices consists of 3 components:

• Saliva Collection Funnel and Collection Tube that contains Norgen’s Preservative in a dried format
• Collection Tube Cap
• ID Label

Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed, the Collection Tube is securely capped with Collection Tube Cap. The collected saliva is mixed by gentle inversion for several times till the saliva colour changes into yellow. The Saliva Collection Tube is subsequently sent to the laboratory for the purification of intact exosomes and for the subsequent isolation and analysis of saliva exosomal RNA. The saliva exosomes in preserved samples are stable for 2 years at room temperature.

Details

Kit Specifications
Volume of Saliva Collected	2 mL
Preservation Temperature	Room Temperature
Preservation time	2 years at room temperature

Shelf Life and Handling

• The Collection Device should be kept tightly sealed and stored at room temperature for up to 2 years without any reduction in kit performance (The collection due date is written on the device label).
• The Collection Tube, the Collection Funnel and the Device Container of each Individual Saliva DNA Collection and Preservation Device are recyclable.

Kit Components	Cat. 65400 (50 Devices)
Individual Saliva Exosome Collection and Preservation Devices	50
Donor Procedure Flowchart	1
Product Insert	1
Individual Saliva Exosome Collection and Preservation Device Contents
Saliva Collection Funnel and Collection Tube	1
Collection Tube Cap	1
Donor Instructions	1