APN-C3-PEG4-alkyne is a heterobifunctional linker containing an APN moiety with exquisite chemoselectivity for cysteine and an alkyne group. The superior stability of APN-cysteine conjugates in aqueous media, human plasma, and living cells makes this new thiol-click reaction a promising methodology for applications in bioconjugation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
APN-C3-PEG4-alkyne is a heterobifunctional linker containing an APN moiety with exquisite chemoselectivity for cysteine and an alkyne group. The superior stability of APN-cysteine conjugates in aqueous media, human plasma, and living cells makes this new thiol-click reaction a promising methodology for applications in bioconjugation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
DL6MB large capacity refrigerated centrifuge
Product Info
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Product Info
DL6MB large capacity refrigerated centrifuge
Features:
1. Widely used in the field of blood bank, pharmaceutical factory and laboratory.
2. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.
3. Digital display which indicates the program, speed, time,RCF, temperature.
4. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration and deceleration for your choice.
5. There are 6 kinds of rotors for your choice.
6. Electric lid lock, compact design, super speed and imbalance protection.
7. The centrifuge body is made of high-quality steel, safe and reliable.
DL6MB Technical Parameter:
Max. Speed
6000rpm
Max. RCF
6600*g
Max. Capacity
6×1000ml(6*500ml blood bag)
Time Range
0~9h59min
RPM/RCF Convert
Yes
Noise (dB)
≤ 55
Temperature
-20-40 degrees
Acc/Dcc
10 Kinds
Speed Accuracy
±20r/min
Temperature Accuracy
±1℃
Voltage(V/Hz)
AC 220V 50HZ/60HZ
Size (W x D x Hmm)
800×675×830mm
Net Weight(Kg)
310KG
Certificates
CE,ISO & Calibration report are available
Matched Rotors for DL6MB
Order No.
Rotor No.
Max Speed (rpm)
Max Volume(ml)
Max.RCF(×g)
No30298
Swing Rotor
4200
6×1000ml
5100
NO30221
Angle Rotor
6000
4×300ml
5390
NO30222
Angle Rotor
6000
6×300ml
5660
NO30223
Angle Rotor
6000
6×500ml
6600
Document
Widely used in the field of blood bank, pharmaceutical factory and laboratory.
HiPure FFPE Nucleic acid Kit supplies a simple and rapid DNA extraction for formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, southern blot and viral DNA detection, etc.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from FFPE tissue samples
Applications
PCR, southern blot and viral DNA detection, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples
Sample amount
<20mg
Elution volume
>15µl
Time per run
≤20 minutes
Liquid carrying volume per column
4ml
Binding yield of column
100μg
Principle
Hipure FFPE Nuclear acid kit adopts silica gel column purification. The sample is deparaffinated by xylene and digested by lysate and protease. After de crosslinked at 90 ℃, DNA/RNA is released into the lysate. Adding ethanol to adjust the binding conditions, the sample is transferred to the column where DNA/RNA is adsorbed on the membrane and protein is removed without adsorption. Protein and other impurities are washed by buffer GW1, and the salt is removed by buffer GW2. Finally, the DNA / RNA is eluted by low salt buffer.
Advantages
Safety – deparaffinating without contacting with xylene or other toxic solution
Fast – without overnight incubation and digestion, several samples can be extracted within 2hours
High efficiency -remove the formaldehyde modification of DNA, greatly enhancing the sensitivity of PCR
Kit Contents
Contents
D312602
D312603
Purification Times
50 Preps
250 Preps
HiPure DNA Mini Columns I
50
250
2ml Collection Tubes
50
250
Buffer DPS
30 ml
150 ml
Buffer ATL
15 ml
60 ml
Buffer AL
15 ml
60 ml
Buffer GW1*
22 ml
88 ml
Buffer GW2*
12 ml
50 ml
Proteinase K
24 mg
120 mg
Protease Dissolve Buffer
1.8 ml
10 ml
Buffer AE
10 ml
30 ml
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
Document
HiPure FFPE Nucleic acid Kit supplies a simple and rapid DNA extraction for formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, southern blot and viral DNA detection, etc.
This kit provides a fast and simple spin column procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. 49000), as well as fresh saliva samples.
Saliva DNA purified using Norgen’s Saliva DNA Isolation Kit Dx kit is of the highest quality, and can be used with any downstream in vitro diagnostic application employing enzymatic amplification or other enzymatic modifications of DNA followed by signal detection or amplification.
Background
Saliva represents an excellent non-invasive alternative to blood collection. Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples.
NOTE: This product is not available for sale in the United States.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. All solutions and plastics can be used until the expiration date specified on their labels. The Proteinase K can be stored at either room temperature or 4oC.