APN-C3-PEG4-alkyne

General information

Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.

Kit contents

1. Champion™ Competent Cells
2. pUC19 Control Plasmid (5 μl, 10-4 μg/μl)
3. Champion™ Transformation Protocol Card

Shipping condition

Throughout the shipping process, the temperature is maintained under -70°C. 

Storage and expiration 

Champion™ Competent Cells must be stored between -70°C to -80°C. Subsequent freeze-thaw cycles will reduce transformation efficiency. If high efficiency is required for the experiment, do not use aliquots that have gone through several freeze-thaw cycles. The efficiency of Champion™ Competent Cells lasts for 1 year with proper storage.

Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.

Description

This fast screening test kit contains 10 tests with everything needed for accurate results of unsafe lead levels in the field. 

This is a revolutionary product designed to make Lead testing in water safe, easy, and affordable.  This fast screening test kit contains 10 tests with everything needed for accurate results of unsafe lead levels. With reliable results in only 10 minutes, this test kit clearly gives results confirming Lead in water and conforming to the EPA guideline of 15 ppb (µg/L). The kit can test water quality from various sources including faucets, wells (ground water), and home purification systems.

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Standards and Regulations for Lead [ACCLPP 2012]

• EPA: Drinking Water: 15ppb
• FDA: Juice: 50ppb
• EPA: Residential Soil: 400 ppm (play areas), 1200 ppm (non-play areas)
• CPSC: Paint: 90ppm
• FDA: Bottled drinking water: 5ppb

High Blood lead levels (i.e., greater than 700ppb) can cause serious health effects, including seizure, coma, and death.  Blood levels as low as 100ppb have been associated with adverse effects on cognitive development, growth, and behavior among children aged 1-5 years.

Sample method:

1. Take a first-draw sample

Immediately after opening a faucet or valve, collect a 250 mL sample. This sample should be from each tap used for consumption.

2. Take a flush sample

If first-draw sample results show elevated lead levels of 5 ppb or higher, collect a flush sample. To do this, ensure water has not been used for between 8 to 18 hours, then collect the sample at 30 seconds.

3. Take sequential samples

If you want to test a lead service line, collect 8 to 10 sequential samples, depending on how far the line is from the tap.

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• CDC – Centers for Disease Control and Prevention
  CPSC – Consumer Products Safety Commission
  EPA – Environmental Protection Agency
• FDA Takes Steps to Limit Lead in Juice to Further Reduce Exposure to Toxic Elements in Foods

Screening of Lead in water samples 5-10 ppb
Format: 10 tests (5 tests/5 controls)
Run Time: 15 Minutes