APN-C3-PEG4-alkyne is a heterobifunctional linker containing an APN moiety with exquisite chemoselectivity for cysteine and an alkyne group. The superior stability of APN-cysteine conjugates in aqueous media, human plasma, and living cells makes this new thiol-click reaction a promising methodology for applications in bioconjugation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
APN-C3-PEG4-alkyne is a heterobifunctional linker containing an APN moiety with exquisite chemoselectivity for cysteine and an alkyne group. The superior stability of APN-cysteine conjugates in aqueous media, human plasma, and living cells makes this new thiol-click reaction a promising methodology for applications in bioconjugation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Ready to Use CdSe/ZnS Qdots Nanoparticle Streptavidin Conjugate for Lateral Flow
Our Streptavidin CdSe/ZnS Quantum Dots (QDs) conjugate is manufactured using special conjugation technology and functonally tested by lateral flow. We have coated our high quality nanoparticles with a proprietary surface coat that covalently binds the biotin forming ultra stable conjugates. The resulting Streptavidin CdSe/ZnS QDs can irreversibly bind biotin.
fluorescent broad range UV light excitation range of 100nm to 400nm, 655nm emission
Highly stable and uniform Streptavidin CdSe/ZnS Qdots in a Ready To Use format
Used for the selective isolation and culture of Listeria monocytogenes.
Columbia Blood Agar Base provide carbon and nitrogen sources, vitamins and growth factors，maintains balanced osmotic pressure; Listeria hydrolyzes aesculin and reacts with iron ions to form black 6,7-dihydroxycoumarin; lithium chloride and other antibiotics can inhibit Gram-negative bacteria and most Gram-positive bacteria; agar is the coagulant of the culture medium.
| Ingredients | /liter |
| Columbia blood agar base | 39.0 g |
| Aesculin | 1.0 g |
| Ferric ammonium citrate | 0.5 g |
| Lithium chloride | 15.0 g |
| pH7.0±0.2 at 25°C | |
Weigh 55.5 g of this product, add 1000 mL of distilled water or deionized water, stir, heat and boil until completely dissolved, divide into Erlenmeyer bottles, sterilize at 121℃ for 15 min. Cool to about 50℃, add 1 bottle of SR0500 supporting reagent A and 1 bottle of B per 100 mL, mix well and set aside.
The following quality control strains were inoculated and cultured at 35-37℃ for 24h. The results are as follows:
| Quality control strains | Growth |
| Listeria monocytogenes ATCC19115 | Gray-green colonies with a black depression in the center and black surrounding |
| Enterococcus faecalis ATCC29212 | – |
| Escherichia coli ATCC25922 | – |
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Intended Use Used for the selective isolation and culture of Listeria monocytogenes. Principle and Interpretation Columbia Blood Agar Base provide carbon and nitrogen sources, vitamins and……
Magnetic Beads (tRNA Purification)
Solid Phase Reversible Immobilization magnetic beads has been used for nucleic acids purification because they are effective, simple, and fast. The carboxyl groups coated beads are paramagnetic particles that reversibly bind to nucleic acid. However, there is a drawback for magnetic beads that it can only purify DNA/RNA that are 100 base pairs or longer. tRNA and other DNA/RNA fragments shorter than 100 base pairs are not effectively recovered. We have developed Magnetic Beads (tRNA Purification) to overcome the hurdle.
The Magnetic Beads (tRNA Purification) solves the tRNA and short DNA/RNA purification problem. The beads with our proprietary technology purify tRNA effectively by removing impurities and unwanted components such as dNTPs, detergents, salts, proteins, and other contaminants. The magnetic bead reagents can be used for oligo (>70 nt) applications.
Workflow without large RNA/DNA contamination
In the case of samples contaminated with RNA/DNA such as rRNA and DNA, our magnetic beads can effectively remove RNA/DNA that are 180 nt and larger. Purified tRNA are ideal for applications requiring high quality, as the fragments are free of impurities and contaminants.
Workflow with large RNA/DNA contamination
Comparison of tRNA and 70 nt oligos recovery. BioDynami beads (tRNA purification) successfully recovered DNA fragments both yeast tRNA and 70 nt oligos.
Recovery of tRNA and 70 nt oligos with BioDynami magnetic Beads (tRNA Purification). Yeast tRNA and 70 nt oligos were used as input. Input and recovered oligos were quantified with ssDNA Quantification kit (BioDynami Cat. # 40043) and RNA Quantification kit (BioDynami Cat. # 40044).
Depletion of larger RNAs. Left panel: depletion of 28S rRNA and 18S rRNA. Right panel: depletion of RNA of 180 nt and larger.
Features
Removal of unwanted components and other impurities
Purification of tRNA and oligos (>70 nt)
tRNA
RNA fragments 70 nt or longer
DNA/RNA hybrid fragments 70 nt or longer
Oligo and chimeric oligo 70 nt or longer
dsDNA fragments 70 bp or longer
ssDNA fragments 70 nt or longer
Removal of larger RNA/DNA contamination:
18S rRNA
28S rRNA
RNA/DNA> 180 nt
Solid Phase Reversible Immobilization magnetic beads has been used for nucleic acids purification because they are effective, simple, and fast. The carboxyl groups coated beads are paramagnetic particles that reversibly bind to nucleic acid. However, there is a drawback for magnetic beads that it can only purify DNA/RNA that are 100 base pairs or longer. tRNA and other DNA/RNA fragments shorter than 100 base pairs are not effectively recovered. We have developed Magnetic Beads (tRNA Purification) to overcome the hurdle.
