Very rapid reaction due to inclusion of galactose mutarotase (patented technology)
Very cost effective
All reagents stable for > 2 years after preparation
Only enzymatic kit available
Very specific
Simple format
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Other Products
C13111 HiPure RNA Mini Column
Product Info
Document
Product Info
Introduction
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
The series of nucleic acid columns produced by Magen Biotech are based on carefully selected imported glass fiber membranes (GF/B, GF/D, GF/F). Columns production processes such as polypropylene injection molding materials, injection molding process, and downstream membrane packing and compression rings are strictly controlled. This is to ensure that the column has extremely high adsorption capacity and long-term stability. Compared with conventional products on the market, Magen’s columns are with varieties, and binding rate will not change when stored at room temperature for 4 years.
Details
Specifications
Features
Specifications
Recommended application
Plasmid Mini Preparation, gDNA/ RNA Extraction,DNA/RNA Clean Up
Preservation conditions
Room temperature
Stability
Up to 4 years
Filter membrane
High quality glass fiber filter GF/B, 3 layers
Membrane aperture
1.0μm
Maximum binding yield of plasmid
30 μg
Maximum yield of alcohol mediated Binding
200 μg
Single liquid carrying capacity of column
800 μl
Minimum elution volume
30 μl
Withstand centrifugal force
16,000 x g
Centrifuge
Small high speed centrifuge (2ml)
Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silicagel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
Ordering information
CAT.No.
Product Name
Package
C13111
HiPure RNA Mini Column (3 x GF/B)with 2ml Collection Tubes
1000/Bag
Purchase Guide
Item No.
Product Name
Membrane type/number of layers
Collection tubes
Plasmid DNA binding capacity (Physical adsorption)
Note: GF/B pore size is for 1.0μM glass fiber membrane; GF/F pore size is for 0.7μm glass fiber membrane.
Document
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
The Primerdesign genesig Kit for Shigella species (Shigella_spp) genomes is designed for the in vitro quantification of Shigella_spp genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design.
The dynamics of genetic variation means that new sequence information may become available after the initial design. Primerdesign periodically reviews the detection profiles of our kits and when required releases new versions.
The target sequence is within the virulence plasmid pCP301 (VirA) which is carried by nearly all clinical isolates of shigella. This target has previously been shown to be a good genetic marker for Shigella in other real time PCR based studies (Hiroshi Fukushima et.al 2003.) The primers and probe sequences in this kit have 100% homology with over 95% of reference sequences in the NCBI database based on a comprehensive bioinformatics analysis.
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Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
Fast and easy high throughput processing using either a vacuum manifold or centrifugation
Process all soil types including clay, loam, sandy soils and high humic content soils such as peat, compost and manure
Remove organic substances using the OSR Solution
Remove all humic acid from DNA samples using the Humic Acid Removal plate
Isolate high quality total DNA from all soil types – ready for any downstream PCR, qPCR
Excellent DNA for metagenomic studies
No phenol or chloroform extractions
This kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from all types of soil samples including common soil samples and difficult soil samples with high humic acid content such as compost and manure. A combination of chemical and physical homogenization effectively lyses all microorganisms in the soil sample, and the kit removes all traces of humic acid using the provided Organic Substance Removal (OSR) Solution and Humic Acid Removal plate (HAR). Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR and qPCR applications and more for any metagenomic study, as all humic acid substances and PCR inhibitors are removed during the isolation.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
