Very rapid reaction due to inclusion of galactose mutarotase (patented technology)
Very cost effective
All reagents stable for > 2 years after preparation
Only enzymatic kit available
Very specific
Simple format
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Other Products
DBCO-PEG24-Maleimide
Product Info
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Product Info
DBCO-PEG24-Maleimide is a PEG linker containing a DBCO moiety and a terminal primary maleimide group. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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DBCO-PEG24-Maleimide is a PEG linker containing a DBCO moiety and a terminal primary maleimide group. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG10-acid is a propargyl PEG linker with a terminal carboxylic acid. The terminal carboxylic acid forms amide bonds with primary amines; activators (EDC or HATU) will be needed. The propargyl group can react with azide-bearing compounds to form stable triazole bonds. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG10-acid is a propargyl PEG linker with a terminal carboxylic acid. The terminal carboxylic acid forms amide bonds with primary amines; activators (EDC or HATU) will be needed. The propargyl group can react with azide-bearing compounds to form stable triazole bonds. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA spin column to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.
Details
Specifications
Features
Specifications
Main Functions
Co-isolation DNA and RNA from skin, muscle, connective tissue, fibrous tissue sample
Applications
PCR and southern blot, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Cultured cells and tissue samples
Sample amount
Tissue: < 20mg
Principle
The Kits are designed to purify both genomic DNA and total RNA from the same cellor tissue sample. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column and bind DNA. Ethanol is added to the flow-through and the sample is applied to an RNA column. DNA/RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit. High-quality DNA is eluted in as little as 50µl water using the Kit.
Advantages
High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
Fast – column method can complete the extraction of several samples in 30 minutes
Safe – no phenol chloroform extraction required
Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
Kit Contents
Contents
R511402
R511403
Purification Times
50 Preps
250 Preps
HiPure DNA Mini Columns
50
250
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
100
2 x 250
Buffer RL
30 ml
150 ml
RNA Digestion Buffer
15 ml
80 ml
Buffer DW1
30 ml
150 ml
Buffer RW1
50 ml
200 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml
Buffer AE
10 ml
50 ml
Storage and Stability
HiPure DNA/RNA Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.
Document
This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA spin column to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.