ArcticZymes Proteinase is an unspecific endopeptidase originating from an Arctic marine microbial source. It has broad substrate specificity and is easy to inactivate after use.
Histones and other proteins are known to protect nucleic acids from interacting optimally with other DNA binding proteins and enzymes. ArcticZymes Proteinase is ideally suited for transforming chromatin and other dense nucleic acids to naked DNA. The enzyme is easy to heat-inactivate. This allows thermal inactivation at temperatures allowing RNA integrity as well as avoiding dissociation of dsDNA.
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ArcticZymes Proteinase is an unspecific endopeptidase originating from an Arctic marine microbial source. It has broad substrate specificity and is easy to inactivate after use.
Histones and other proteins are known to protect nucleic acids from interacting optimally with other DNA binding proteins and enzymes. ArcticZymes Proteinase is ideally suited for transforming chromatin and other dense nucleic acids to naked DNA. The enzyme is easy to heat-inactivate. This allows thermal inactivation at temperatures allowing RNA integrity as well as avoiding dissociation of dsDNA.
Key Features
Easy to inactivate after use: 15 – 30 min at 55 – 60°C
Broad substrate specificity
Active at high salt concentrations
Compatible with downstream DNA analyses
Available in Glycerol FREE formulation
Applications
Lysis and releasing nucleic acid from single-cells and small tissue samples
Optimization of DNA/RNA yields from nucleic acids when added to extraction procedures
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Isotherm3G DNA Polymerase utilizes an aptamer-based warm-start feature to prevent false amplification at low temperatures. For more information, please contact us.
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Document
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Urine Total RNA Purification Maxi Kit Dx (Slurry Format)
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Overview
Isolate high quality total RNA and all sizes of circulating and exosomal RNA, including microRNA
No phenol extractions
Very sensitive and linear without the need for carrier RNA
Bind and elute all RNA irrespective of size or GC content, without bias
Isolate inhibitor-free urinary RNA
Slurry/Spin column and Slurry/96-well format available
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Urine Total RNA Purification Maxi Kit Dx (Slurry Format) provides a fast, reliable and simple procedure for isolating total RNA from urine for subsequent in vitro diagnostic use. Purification is based on the use of Norgen’s proprietary resin as the separation matrix, and the kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to small RNAs.
This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Urine Total RNA Purification Maxi Kit Dx (Slurry Format) in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.
To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.
Norgen’s Urine Total RNA Purification Maxi Kit Dx (Slurry Format) is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques including RNA isolation.
Norgen’s Urine Total RNA Purification Maxi Kit Dx (Slurry Format) does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable until the expiration date specified on the label.
