25 tests This Rapid Test is for the detection of specific Aspergillus Galactomannan Antigen in Serum or Bronchoalveolar Lavage (BAL).
This product is manufactured by GaDia Diagnostics in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Detail
Name of Product
Aspergillus Antigen (Galactomannan) – Rapid Test
Catalog Number
ASP-025
Short Info
This Rapid Test is for the detection of specific Aspergillus Galactomannan Antigen in Serum or Bronchoalveolar Lavage (BAL).
This product is manufactured by GaDia Diagnostics in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
Immunochromatographic Rapid Test
Range/Assay Sensivity
Test Principle
The principle of the test is colloidal gold immunochromatography. If the sample is positive, the antigens in the sample react with the red-colored nanoparticles and form a complex (Antigen – anti-Aspergillus monoclonal antibodies – gold nanoparticles), which was previously pre-dried on the conjugate pad. The mixture then moves upward on the membrane by capillary action. As the sample flows through the test membrane, the binding conjugate complexes migrate. The anti-Aspergillus antibodies present on the membrane (Test Line) capture the colored conjugate complex and a red line will appear.
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 250-500mg soil sample
Applications
PCR, southern blot and enzyme digestion, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Soil
Sample amount
250-500mg
Elution volume
≥50μl
Time per run
≤70 minutes
Principle
This product is based on the purification method of high binding magnetic particles. Soil sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our propietary Absorber Solution. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
High concentration – maximum extraction of total DNA from soil samples
High purity – purified DNA can be directly used in various downstream applications
Kit Contents
Contents
D635601
D635602
D635603
Purification Times
48 Preps
96 Preps
5 x 96 Preps
MagPure Particles
2.5 ml
5 ml
22 ml
2ml Bead Tubes
48
96
400
Buffer SOL
60 ml
100 ml
500 ml
Buffer SDS
6 ml
10 ml
50 ml
Reagent DX
1 ml
1 ml
5 ml
Buffer PS
10 ml
20 ml
90 ml
Absorber Solution
10 ml
20 ml
90 ml
Buffer GDP
70 ml
150 ml
2 x 350 ml
Buffer GW1*
22 ml
44 ml
220 ml
Elution Buffer
20 ml
20 ml
60 ml
Storage and Stability
MagPure Particles and Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
Document
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.
A universal PCR master mix for allele-specific PCR assays. Precision fluorescent signal generation with consistently high performance at any reaction volume.
PACE Genotyping Master Mix uses a novel, universal, fluorescent reporting cassette to produce machine-readable fluorescent signals corresponding to genotypes. PACE compatible genotyping assays are comprised of two competitive allele specific forward primers (which differ in their terminal 3’ bases and unique 5’ tail sequences) and a common, reverse primer. PACE Genotyping Master Mix is supplied at 2x concentration and with ROX normalising dye at a range of levels to ensure compatibility with your qPCR instrument or reader.
Genotyping assay designs are available from 3CR Bioscience through our free PACE assay-design service; once designed, users can purchase assay primers independently or through 3CR Bioscience using our partial or full-assay validation service. PACE Genotyping Master Mix is also compatible with KASP™ and Amplifluor® marker assays.
REQUIRED COMPONENTS
qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal
Template DNA
PCR-grade water
Genotyping assays
Document
qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal
DBCO-PEG4-C3-sulfonic acid serves as a bifunctional PEG linker. The DBCO click chemistry handle conjugates with azides on target molecules to form stable triazole linkages. The sulfonic acid can participate in esterification, halogenation and displacement reactions.
Document
DBCO-PEG4-C3-sulfonic acid serves as a bifunctional PEG linker. The DBCO click chemistry handle conjugates with azides on target molecules to form stable triazole linkages. The sulfonic acid can participate in esterification, halogenation and displacement reactions.
