12 x 8 strips (96 tests)
This ELISA kit is intended for the quantitative detection of IgG antibodies against Aspergillus fumigatus in human Serum.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Detail
Name of Product
Aspergillus fumigatus – IgG ELISA
Catalog Number
AF 6100
Short Info
This ELISA kit is intended for the quantitative detection of IgG antibodies against Aspergillus fumigatus in human Serum.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
pNPP, λ=405 nm
Test Principle
Aspergillus fumigatus specific antibodies in the sample bind to Aspergillus fumigatus antigens sensitized on microtiter plates. The presence of fungal specific antibodies is detected with a Protein A – alkaline phosphatase conjugate.
One Step Isothermal Basic RNA Amplification Kit For High Throughput RNA Analysis
Product Info
Document
Product Info
Product Description
One-Step Isothermal Amplification Basic Kit for High Throughput RNA Analysis
Product Detail
Kit Storage and Term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal Nucleic Acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature:at room temperature and constant temperature(generally 39℃~42℃),reverse transcriptaseuses specific primers and template RNA tosynthesize cDNA strands,and binds the auxiliary protein and single strand with the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology positionand strand exchange begins; accompanied by the recombinase from the complex upon dissociation,the polymerase also bindsto the 3′ end of the primer,initiating chainextension.It is suitable for laboratory-level RNA amplification and RNA amplification for other detection purposes.
Technical Parameters:
Parameters
Details
Product Name
RNA Isothermal Amplification Kit Basic
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal Nucleic Acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Isothermal Nucleic Acid Applications
Suitable for RNA isothermal rapid amplification kit(BASIC type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis
Influenza is caused by three immunologic types of RNA viruses (A, B and C) within the Orthomyxoviridae family. Seasonal influenza is typically caused by three major subtypes of hemaglutinin (H1, H2 and H3) and two subtypes of neuraminidase (N1 and N2). A novel sub-type of influenza A virus called pandemic H1N1 2009 virus was identified in Mexico and reported by the CDC and WHO in April, 2009 (Novel swine-origin influenza A (H1N1) virus investigation team, 2009; CDC, 2009; and Fraser et al., 2009). H1N1 2009 is a novel sub-type virus that transmits easily between humans with 21 countries reporting cases within a month of initial identification (CDC, 2009-b). It is essential that public health laboratories around the world undertake detailed surveillance to monitor the spread and impact of pandemic H1N1 2009 virus as well as try to predict future changes in virulence (Fraser et al., 2009). Methods for the rapid diagnosis, case identification and tracking of this novel pathogen in the human population are therefore required to develop appropriate management strategies to mitigate morbidity and mortality.
H1N1 TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
H1N1 TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Convenient optimized on-column DNase treatment using Norgen’s RNA Purification Kits
Also includes protocol for digestion in-solution followed by RNA Clean-Up
Guaranteed RNase-Free
Includes Enzyme Incubation Buffer
Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600 units/vial)
Norgen’s RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen’s RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen’s RNA purification kits. Alternatively, after isolating total RNA using one of Norgen’s RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen’s RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.
Details
Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.
Storage Conditions The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.