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BASIC AND ADVANCED LEAF CUTTING TOOL

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Efficient, leaf punch tools for quick, precise field sampling. For use with the 3CR Bio Leaf Sample Collection Kit.

Detail

Efficient, leaf punch tools for quick, precise field sampling. For use with the 3CR Bio Leaf Sample Collection Kit.

About

BASIC LEAF CUTTING TOOL

The 3CR Bio Basic Leaf Cutting Tool is a lightweight, easy-to-use, low-cost punch designed for precise sampling of thin or soft materials, ideal for fieldwork. This tool is expertly designed to core, punch, and retrieve samples from thin or soft source materials such as leaves. The tool comprises a convenient, hollow plastic pen-style holder, mounted with a durable stainless steel cutting tip at one end. It features an internal push-button-operated plunger for safe and efficient sample disc ejection. The tool produces a precise ¼ inch diameter disc, making it ideal for detailed sampling tasks. For best results, clean the cutting tip between each sample extraction by coring blank filter paper or rinsing with water or ethanol. This low-cost leaf punch is lightweight, easy to use, and perfect for fieldwork.

ADVANCED LEAF CUTTING TOOL

The 3CR Bio Advanced Leaf Cutting Tool is designed to streamline and enhance your sampling process. This high-precision leaf tissue punch produces a single ¼ inch diameter disc effortlessly. It features a unique mechanism that securely holds a standard snap-top or screw-top 2 mL storage tube in a fixed position, allowing the sample to be received without any need for touching or manipulation. Ideal for fieldwork, this advanced leaf punch significantly reduces effort and time when performing multiple samplings, ensuring accuracy and efficiency in every use.

Other Products

Description

DNA amplification made possible with a TwistAmp® Basic kit. Contains all the enzymes and reagents necessary for the amplification of DNA, the user needs only supply primers and template. Even PCR primers can work using TwistAmp® Basic. TwistAmp® Basic has been used for solid-phase, tailed primers, aptamers, electrochemistry and microarray applications. See manual for more information. Click to order oligonucleotides.

Perfect for: End-point gel electrophoresis DNA detection, down-stream applications (e.g. sub-cloning)

[QP3510] Q-PAGE™ Bis-Tris Precast Gel (Midi, 12 wells, 4-12%), 10 gels

Description 

Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels. 

Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.

Key Features

  • User-friendly gel cassette:
    • Numbered and framed wells for sample loading
    • With cassette opener for easy use
  • Enhanced gel performance:
    • Enhanced band sharpness
    • Better resolution of small proteins
    • Stable for shipping at ambient temperature
  • Easy compatibility: 
    • Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
    • Compatible with most popular protein electrophoresis systems 

Storage and stability

Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.

Do not freeze Q-PAGE™ Precast Gels Remove tape and comb before electrophoresis. 

Keep Q-PAGE™ Precast Gels flat during storage.

cf-DNA/cf-RNA Preservative Tubes Dx

Overview

  • CE-IVD marked in accordance with EU Directive 98/79/EC
  • Ideal for use in in vitro diagnostic workflows
  • Fixative-free preservative, no cross-linking of DNA
  • Preserve cf-DNA/ct-DNA for 30 days at ambient temperature and for up to 8 days at 37°C.
  • Preserve cf-RNA for 30 days at ambient temperature
  • Preserve Circulating Tumour Cells (CTCs) for 14 days at ambient temperature
  • No plasma volume loss after shipping/transportation
  • Prevent hemolysis allowing better separation of plasma
  • Prevent apoptosis of blood cells and fragmentation of genomic DNA
  • Produce high quality/quantity of plasma cf-DNA/ct-DNA/cf-RNA
  • Vacuumed to draw 8.4 mL of blood in 10 mL tubes
  • The procedure for plasma isolation using this kit is compliant with ISO 20186-3:2019

Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx are closed, evacuated plastic tubes for the collection and the preservation of cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and shipping. Using these tubes in combination with Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen’s Plasma/Serum RNA Purification Kits enables the purification and concentration of an inhibitor-free cf-DNA/ct-DNA/cf-RNA in a very small elution volume. 

These tubes are ideal for collecting and preserving cf-DNA and cf-RNA samples for in vitro diagnostic use for medical purposes.

Performance

Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx abridge the collection/processing of whole blood for the subsequent purification of cf-DNA and/or cf-RNA. The cf-DNA/cf-RNA Preservative Tubes Dx preserve and inhibit the programmed cell-death (apoptosis) of the blood, hence preventing the release of intracellular DNA/RNA into plasma. Whole blood samples can be shipped and stored at room temperature for the subsequent processing of plasma. The cf/ct DNA and cf-RNA levels are stable for up to 30 days at room temperature. The cf-DNA is also stable for up to 8 days at 37°C.

Workflow

NOTE: This product is not available for sale in the United States. For the research use only version, see Cat. 63950

Details

Supporting Data

Figure 1 / 7

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Figure 1. Effect of ambient temperature storage
Figure 1. Effect of ambient temperature storage
Figure 2. No plasma volume loss after shipping/transportation
Figure 3. Hemolysis of collected blood measured over time
Figure 4. Prevent cell lysis and the release of gDNA and accumulation of apoptotic ladder in plasma
Figure 5. Effect of high temperature (37 C) storage for 8 days
Figure 6. High Quantity of cf-DNA from Plasma preserved
Figure 7. Effect of ambient temperature storage

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Kit Specifications
Tube Size16 x 100 mm
Tube TypePET Tube
Blood Draw Volume8.4 mL in 10 mL Tubes
Preservative Volume1.6 mL
Recovered Plasma VolumeApproximately 6-7 mL
(not affected by shipping)
AnticoagulantProprietary
PreservativeNon formaldehyde – Proprietary preservation agent
Length of cf-DNA PreservationUp to 30 days at room temperature (15-25°C)
Up to 8 days at 37°C
Length of cf-RNA PreservationUp to 30 days at room temperature (15-25°C)
Length of CTCs PreservationUp to 14 days at room temperature (15-25°C)


Storage Conditions and Product Stability

  1. When stored at 15-30°C, unfilled Norgen cf-DNA/cf-RNA Preservative Tubes Dx are stable through the expiration date.  See tube label for expiry date.
  2. Short-term storage/shipping at -20°C to 37°C for up to 10 days is acceptable for unfilled Norgen cf-DNA/cf-RNA Preservative Tubes Dx.
  3. Do not freeze unfilled Norgen cf-DNA/cf-RNA Preservative Tubes Dx for long term storage over 10 days. Freezing unfilled tubes may lead to loss of vacuum and precipitation may occur.
  4. Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx is stable for up to 30 days when stored at room temperature (15-25°C)
  5. Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx is stable for up to 8 days when stored at 37ºC.
  6. Cell-free RNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx is stable for up to 30 days when stored at room temperature (15-25°C)
  7. Circulating tumor cells (CTCs) in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx are stable for up to 14 days at room temperature (15-25ºC).
ComponentCat. Dx63950 (50 tubes)
cf-DNA/cf-RNA Preservative Tubes Dx50
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