m-PEG2-DBCO is a monodisperse PEG reagents which can enable copper-free Click Chemistry through the reaction of DBCO with azide. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Product Description

Product Detail

Kit Storage and Term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal Nucleic Acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3′ end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

Isothermal Nucleic Acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	RNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal Nucleic Acid Applications

Suitable for RNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

 

Overview

• Detection kits for Trichomonas vaginalis
• Available in TaqMan format for analysis

Norgen’s Trichomonas vaginalis End-Point PCR Kit is designed for the detection of Trichomonas vaginalis specific DNA based on the use of end-point PCR technology. This kit is designed for research use only and not for use in diagnostic procedures. The kit includes Master Mix and primers for the specific amplification of a 335 nucleotide region of the Trichomonas vaginalis genome, as well as a positive control and a negative control to confirm the integrity of the kit reagents. In addition, the kit contains loading dye and a DNA ladder to facilitate analysis of the results.

The detection of Trichomonas vaginalis specific DNA is based on end-point PCR technology, utilizing polymerase chain reaction (PCR) for the amplification of specific Trichomonas vaginalis DNA sequences. For analysis of the PCR data, the PCR reaction is loaded on an agarose DNA gel along with the provided DNA ladder for qualitative analysis.

Trichomonas vaginalis TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Trichomonas vaginalis TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

Figure 1 / 3

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM52050 (100 preps)	Cat. TM52010 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Trichomonas vaginalis Primer & Probe Mix	280 μL	280 μL
Trichomonas vaginalis Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1