Biotin-PEG12-DBCO is a biotinylating reagent comprised of DBCO, a click chemistry handle that is frequently used to conjugate with azides on target molecules, and biotin, which is useful for affinity-based applications such as pull-down assays or for ligating with streptavidin proteins. The PEG12 imparts the hydrophilicity of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Biotin-PEG12-DBCO is a biotinylating reagent comprised of DBCO, a click chemistry handle that is frequently used to conjugate with azides on target molecules, and biotin, which is useful for affinity-based applications such as pull-down assays or for ligating with streptavidin proteins. The PEG12 imparts the hydrophilicity of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
Mal-amido-PEG4-alkyne
Product Info
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Product Info
Mal-amido-PEG4-alkyne is a short PEG linker featuring a maleimide and a terminal alkyne. Maleimide is a covalent ligand used for linking thiols such as those in cysteines residues in proteins, while terminal alkynes can be used in copper (I) click chemistry with azides on target molecules.
Document
Mal-amido-PEG4-alkyne is a short PEG linker featuring a maleimide and a terminal alkyne. Maleimide is a covalent ligand used for linking thiols such as those in cysteines residues in proteins, while terminal alkynes can be used in copper (I) click chemistry with azides on target molecules.
endo-BCN-PEG2-amine is a heterobifunctional PEG linker containing a BCN and an amine group. The BCN group can react with azide-tagged molecules. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
endo-BCN-PEG2-amine is a heterobifunctional PEG linker containing a BCN and an amine group. The BCN group can react with azide-tagged molecules. The amino group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
110 mL of prepared reagent (e.g. 500 assays of 0.22 mL)
Content:
110 mL of prepared reagent (e.g. 500 assays of 0.22 mL)
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Acetic Acid
Assay Format:
Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
up to 1.8 g/L of acetic acid per assay
Limit of Detection:
~ 10 mg/L
Reaction Time (min):
8 min at 25oC or 5 min at 37oC
Application examples:
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables, pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products (and baking agents), ketchup, soy sauce, mayonnaise, dressings, paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions), feed and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Improved method
The Acetic Acid GK format test kit is for use with auto-analysers and is suitable for the specific measurement and analysis of acetic acid (acetate) especially in wines, fruit juices, beverages and food products.
As part of Megazyme’s overall commitment to providing the highest quality products, we have developed this acetic acid kit that provides a specific and rapid assay for use with auto-analysers. The kit assay is based on the conversion of NAD+ to NADH and therefore provides a positive reaction (increase in absorbance) which offers a more robust assay.
The reagents, as supplied, are stable for a minimum of 2 years and the prepared reagents are stable for a minimum of 1 week (on-board stability). In addition, the prepared reagents can be stored frozen for longer term stability (see K-ACETGK Booklet for more details).
View more of our acetic acid and organic acid assay kits.
Advantages
Excellent reagent stability
> 7 days at 4oC or > 2 years below -10oC when prepared for auto-analyser applications
> 2 years as supplied
Very rapid reaction (~ 5 min at 37oC)
Linear calibration (R2 ~ 0.997 up to 1.8 g/L sample)
Document
The Acetic Acid GK format test kit is for use with auto-analysers and is suitable for the specific measurement and analysis of acetic acid (acetate) especially in wines, fruit juices, beverages and food products.