Biotin-PEG2-C4-alkyne

Product Description

Comprehensive Monitoring & Early Detection Nucleic Acid Detection Bovine Viral Diarrhea Virus

Product Description:

The Livestock Disease Kit is a reliable and easy-to-use test kit designed to detect the presence of diseases in livestock, such as cattle, sheep, goats, pigs and horses. The kit has been designed to detect diseases with high accuracy and sensitivity. Also it requires only a small sample volume of 50 μl, making it highly efficient and cost-effective. It is also suitable for a range of canine dog test kit applications, providing fast and accurate results.The reliable detection results make it a reliable and cost-effective solution for farmers and veterinarians looking to diagnose livestock diseases quickly and accurately.

Features:

• Product Name: Bovine viral diarrhea virus isothermal detetction fluorescence kit
• Target Species:  Bovine
• Type: Fluorescence Kit
• Storage Temperature: -20°C
• Sample Type: Serum, Plasma, Whole Blood

Technical Parameters:

Applications:

Amp-future bio Livestock Disease Kit is an innovative animal health care product designed to detect and diagnose livestock diseases. Developed by a Chinese research team, this kit is a simple and reliable solution to test and treat livestock diseases. With its detection limit of 0.1 ng/ml, users can obtain accurate results quickly and accurately. The test time takes from 5-20 minutes, and the kit typically contains 48 tests/kit. Furthermore, it is easy to store with its cold storage temperature of -20°C.

The Amp-future bio Livestock Disease Kit provides an innovative solution for animal health care. It is an essential product for veterinary professionals, pet owners, and animal health researchers to detect and diagnose livestock diseases. With its simple and reliable detection and diagnosis, this kit is a must-have for anyone who wants to ensure the health of their animals.

Support and Services:

Livestock Disease Kit Technical Support and Services

We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.

• Our technical support staff is available to answer any questions you may have about the product.
• We offer online tutorials and user guides to help you understand the product better.
• We provide technical assistance to help troubleshoot any issues you may be having with the product.
• We offer a warranty on our products.

If you have any questions or need technical support, please do not hesitate to contact us. We are here to help you make the most of your Livestock Disease Kit.

FAQ:

• Q: What diseases can Livestock Disease Kit detect?
  A: Livestock Disease Kit can detect different infectious diseases, such as Foot and Mouth Disease, Bluetongue Disease, Bovine Viral Diarrhea.
• Q: How does Livestock Disease Kit work?
  A: Livestock Disease Kit uses advanced MIRA technology to amplify the target DNA/RNA targent sequences , and detect the disease by analyzing the amplified sequences.
• Q: How long does it take to get results from Livestock Disease Kit?
  A: Livestock Disease Kit can give a result within 5-20 mins.
• Q: What type of sample is suitable for Livestock Disease Kit?
  A: Livestock Disease Kit is suitable for blood, serum, and tissue samples.
• Q: Does Livestock Disease Kit require professional equipment?
  A: No, Livestock Disease Kit does not require any professional equipment,can use with our isothermal equipment or pcr machine

The Livestock Disease Kit is a reliable and easy-to-use test kit designed to detect the presence of diseases in livestock, such as cattle, sheep, goats, pigs and horses. The kit has been designed to detect diseases with high accuracy and sensitivity. Also it requires only a small sample volume of 50 μl, making it highly efficient and cost-effective. It is also suitable for a range of canine dog test kit applications, providing fast and accurate results.The reliable detection results make it a reliable and cost-effective solution for farmers and veterinarians looking to diagnose livestock diseases quickly and accurately.

What is Collagen?

Collagen is a fundamental component of the extracellular matrix, and the predominant protein in animals, constituting around 30% of total protein mass. A glycoprotein, it is well known for its triple helical structure. This is formed from three polypeptide α-chains with Gly-X-Y repeating residues (Gly for Glycine, X for proline, and Y for hydroxyproline).

‍Types of Collagen

Over 28 types of collagens have been identified, with Type I collagen being the most abundant. It’s prevalent in ligaments, tendons, skin, and bone tissue. Its mature, insoluble form grants it remarkable strength, making it vital for the mobility of organisms. Collagen also has biochemical functions, influencing cell growth, proliferation, and differentiation.

This version of the kit is designed to detect and measure  SOLUBLE forms of collagen. Chose the Sircol Insoluble collagen kit if you need to analyse INSOLUBLE collagen.

Applications of Collagen

Collagen, with its diverse properties, finds utility in various industries. It plays a role in medicine for wound healing and has an expanding role in tissue engineering and cell culture for biomedical purposes. It’s gaining popularity in the cosmetic industry for skin rejuvenation and is used in chemical formulations and the food industry as a functional food supplement and additive.

How does Sircol 2.0 detect collagen?

The Sircol 2.0 dye reagent includes Sirius Red, a linear anionic dye with sulfonic acid side chains. This reagent is specially formulated to bind to the Gly-X-Yn helical structure of soluble collagen under assay conditions.

*The improved formulation of Sircol 2.0 dye enables a greater degree of dye-collagen specificity (compared to our previous S1000 assay kit).

‍Overview of the Sircol 2.0 assay process:

Step 1. Prepared samples are placed in the wells of the assay microplate, together with Sircol Dye Reagent. After 30 minutes mixing, any collagen-dye complexes will form as a precipitate. These are collected on the base of the microplate wells by centrifugation.

Step 2. Unbound dye is removed by gentle aspiration, followed by a rinse with Plate Wash Reagent.

Step 3. Following further centrifugation, collagen-bound dye is eluted by incubation with a Dye Release Reagent. Eluted dye is detected ‘in-situ’ by spectrophotometric analysis of the microplate at 556nm.

Step 4. The collagen content of unknown samples can be quantified by comparison against a calibration curve, prepared using the Collagen Reference Standard supplied with the kit.

A list of suggested sample types can be found under the ‘Assay Specification‘ tab.

Assay range

2 – 200µg/ml or 0.25 – 20µg/ml (using optional Collagen Concentration Protocol)

Limit of Detection

2 µg/ml

Detection Method

Colorimetric Detection (556nm) (Endpoint), Requires a microplate centrifuge.

Measurements per kit

96 in total (allows a maximum of 41 samples to be run in duplicate alongside a standard curve).

Suitable Samples

Soluble* collagens from mammalian**:

In-vivo: Tissues, cartilages and fluids.

‍In-vitro: Extracellular matrices / Conditioned media from 2D/3D culture environments.

The straightforward sample processing and analysis of Sirco 2.0 make it a good alternative to conventional hydroxyproline analysis.

‍*Prior salt/acid/acid-pepsin extraction may be necessary to release soluble collagen.

**Sircol 2.0 is primarily designed for use with in-vivo / in-vitro samples of mammalian origin. Collagens originating from other taxonomic groups and kingdoms can also be analysed. See note on p6 of manual for further information.‍

Precautions

This kit is designed for research use only. Not for use in diagnostic procedures.
Kit requires access to a microplate centrifuge* (see note below), as well as a spectrophotometer/colorimeter capable of absorbance detection at 556nm.
Specific sample preparation protocols may require customer to provide further reagents, consult assay manual for further information.

‍

*As a minimum, we recommend that the centrifuge can centrifuge a 96-well microplate at 400 x g for 120 minutes. Higher speed centrifuges are recommended (up to a maximum of 2000 x g), allowing a reduction in centrifuge time.

Sircol 2.0 kit contents:

1. Dye Reagent (1x20ml)

2. Collagen Reference Standard (1x5ml, 200µg/ml of soluble Bovine collagen)

3. Plate Wash Reagent (1x28ml)

4. Collagen Concentration Reagent (1x25ml)

5. Neutralisation Reagent (1x8ml)

6. Dye Release Reagent (1x25ml)

7. Assay Microplate (1×96-wells)

8. Microplate Seals (6x)

9. Documentation (QuickStart Guide / Manual / Certificate of Analysis)

NB: Additional reagents may be required for sample preparation prior to assay. Consult manual or contact us for further details. This kit requires the use of a microplate centrifuge, capable of centrifuging a 96-well microplate at 400 x g for 120 minutes. Higher speed centrifuges are recommended (up to a maximum of 2000 x g), allowing a reduction in centrifuge time.

Experience user-friendly detection & measurement of Soluble Collagen with Sircol™ 2.0! Our latest kit simplifies collagen quantification within in-vivo / in-vitro samples. Sircol 2.0 offers enhanced sensitivity and accuracy compared to our previous Sircol kit.

Tri(propargyl-PEG5-NHCO-ethyloxyethyl)amine is a click chemistry branched linker. The propargyl groups can react with azide-bearing molecules via copper catalyzed Click Chemistry.

Tri(propargyl-PEG5-NHCO-ethyloxyethyl)amine is a click chemistry branched linker. The propargyl groups can react with azide-bearing molecules via copper catalyzed Click Chemistry.