Biotin-PEG3-alkyne is a biotin PEG reagent with an alkyne group that enables click reaction with azido molecules in the presence of Cu catalyst. The hydrophilic PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule.
Biotin-PEG3-alkyne is a biotin PEG reagent with an alkyne group that enables click reaction with azido molecules in the presence of Cu catalyst. The hydrophilic PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule.
An adapter for Cerillo’s Alto plate reader. This adapter allows the Alto plate reader to fit perfectly on the deck of the Opentrons Flex or OT-2 to allow for pipetting directly into the reader.
An adapter for Cerillo’s Alto plate reader. This adapter allows the Alto plate reader to fit perfectly on the deck of the Opentrons Flex or OT-2 to allow for pipetting directly into the reader.
These kits concentrate urine proteins while simultaneously removing salts, urea, and other urine contaminants. There is no molecular weight cut-off and therefore the columns capture total urinary proteins and peptides of all sizes making them ideal for biomarker discovery work, differential expression of proteins in various diseases, or other diagnostic research. The columns are convenient, rapid and easy to use and thus offer significant time savings over classic dialysis protocols. The resulting high-quality protein sample is concentrated and free from the original sample salts, thus preparing the sample conveniently for downstream proteomic applications including SDS-PAGE, 2D Gels, MALDI-TOF, LC/MS, LC/MS/MS, whole protein mass spectrometry, western blotting, protein microarrays, and more.
ProteoSpin™ Urine Protein Concentration Micro Kit
Each spin column is able to concentrate and desalt up to 200 μg of urine proteins. Twelve samples can be processed in 20 minutes.
ProteoSpin™ Urine Protein Concentration Midi Kit
The ProteoSpin™ Urine Protein Concentration Midi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 1 to 5 mL inputs of urine. Each mini spin column is able to concentrate and desalt up to 3 mg of urine proteins in 30 minutes.
ProteoSpin™ Urine Protein Concentration Maxi Kit
The ProteoSpin™ Urine Protein Concentration Maxi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 2 to 20 mL inputs of urine. Each maxi spin column is able to concentrate and desalt up to 4 mg of urine proteins in 45 minutes.
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| Kit Specifications | |
| Maximum Urine Input Volume | 20 mL |
| Maximum Recovered Protein | 4 mg |
| Minimum Elution Volume | 2 mL |
| Time to Process 4 Samples | 45 minutes |
Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 17400 (25 preps) | Cat. 52300 (10 preps) | Cat. 21600 (4 preps) |
|---|---|---|---|
| Wash Solution C | 60 mL | 60 mL | 130 mL |
| Binding Buffer A | 4 mL | 4 mL | 8 mL |
| Elution Buffer C | 8 mL | 30 mL | 30 mL |
| Protein Neutralizer | 4 mL | 4 mL | 4 mL |
| Micro Spin Columns | 25 | – | – |
| Midi Spin Columns (assembled with collection tubes) | – | 10 | – |
| Maxi Spin Columns (assembled with collection tubes) | – | – | 4 |
| Collection Tubes | 25 | – | – |
| Elution Tubes (1.7 mL) | 25 | – | – |
| Midi Elution Tubes | – | 10 | – |
| Elution Tubes (50 mL) | – | – | 4 |
| Product Insert | 1 | 1 |
N-(t-butyl ester-PEG4)-N-bis(PEG4-Propargyl) is a trifunctional PEG linker that combines a t-butyl ester with two terminal alkynes. The alkynes can be applied in copper-click chemistry with azides to form stable triazole linkages with a target molecule, while the t-butyl ester can be deprotected and reacted with amines or alcohols to form amides or esters.
N-(t-butyl ester-PEG4)-N-bis(PEG4-Propargyl) is a trifunctional PEG linker that combines a t-butyl ester with two terminal alkynes. The alkynes can be applied in copper-click chemistry with azides to form stable triazole linkages with a target molecule, while the t-butyl ester can be deprotected and reacted with amines or alcohols to form amides or esters.
