Bis-PEG11-DBCO

Overview

• Simultaneous isolation of both host DNA and microbial DNA (universal protocol)
• Fully compatible with Norgen’s Stool Nucleic Acid Collection and Transport Tubes
• Eliminates PCR inhibitors including humic acids
• High quality DNA for sensitive downstream applications including PCR, qPCR, Sequencing and microarray
• 96-Well and Magnetic Bead System formats also available

These kits provide a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. Purifies the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA can be used with a number of downstream applications.

Stool DNA Isolation Kit (Spin Column)

Universal method to detect microorganism and host cell DNA simultaneously in stool samples. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.

Stool DNA Isolation 96-Well Kit (High Throughput)

Fast and easy high throughput processing using centrifugation. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.

Stool DNA Isolation Kit (Magnetic Bead System)

Fast and easy processing using a magnetic bead system. Robust lysis system (chemical lysis combined with a mechanical homogenization).

Stool DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

This kit provides fast and easy processing using a magnetic bead system, and a robust lysis system (chemical lysis combined with a mechanical homogenization). High throughput and compatible with an automation robotic system.

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Details

Supporting Data

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Kit Specifications
Maximum Stool Input	200 mg (fresh/frozen stool) or 400 μL (preserved stool)
Type of Stool Processed	Frozen, fresh or preserved stool
Format	Spin Column
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Elution Volume	50 μL
Time to Complete 10 Purifications	30 minutes
Applications	PCR, qPCR, Southern Blot Analysis, Sequencing, Microarray Analysis.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 27600 (50 preps)	Cat. 65600 (192 preps)	Cat. 55700 (50 preps)	Cat. 63100 (192 preps)
Lysis Buffer L	60 mL	2 x 105 mL	60 mL	3 x 60 mL 1 x 30 mL
Lysis Additive A	6 mL	25 mL	6 mL	25 mL
Binding Buffer I	7 mL	25 mL	7 mL	25 mL
Binding Buffer C	30 mL	110 mL	–	–
Wash Solution A	18 mL	2 x 38 mL	–	–
Magnetic Bead Suspension	–	–	1.1 mL	4 x 1.1 mL
Solution WN	–	–	18 mL	55 mL
Elution Buffer B	8 mL	30 mL	8 mL	30 mL
Bead Tubes	50	200	50	196
Mini Spin Columns	50	–	–	–
96-Well Plate	–	2	–	2
Adhesive Tape	–	4	–	2
Collection Tubes	50	–	–	–
96-Well Collection Plate	–	2	–	–
Elution Tubes (1.7 mL)	50	–	50	–
96-Well Elution Plate	–	2	–	2
Product Insert	1	1	1	1

Overview

The iST Scientific extra volume 384-well full-skirted plate enables post-PCR applications such as enzymatic digestions and ethanol precipitation to be carried out in the wells due to the increased well volume.

• Clear in colour
• Polypropylene
• For use in post-PCR applications such as enzymatic digestions and ethanol precipitation
• 384 wells
• Full skirt with cut corner: A24
• Packaged 10 plates per pack, 10 packs per case (100 plates total)

The iST Scientific extra volume 384-well full-skirted plate enables post-PCR applications such as enzymatic digestions and ethanol precipitation to be carried out in the wells due to the increased well volume.