bis-PEG23-endo-BCN

About

A universal PCR master mix for allele-specific PCR assays. Precision fluorescent signal generation with consistently high performance at any reaction volume.

PACE Genotyping Master Mix uses a novel, universal, fluorescent reporting cassette to produce machine-readable fluorescent signals corresponding to genotypes. PACE compatible genotyping assays are comprised of two competitive allele specific forward primers (which differ in their terminal 3’ bases and unique 5’ tail sequences) and a common, reverse primer.  PACE Genotyping Master Mix is supplied at 2x concentration and with ROX normalising dye at a range of levels to ensure compatibility with your qPCR instrument or reader.

Genotyping assay designs are available from 3CR Bioscience through our free PACE assay-design service; once designed, users can purchase assay primers independently or through 3CR Bioscience using our partial or full-assay validation service. PACE Genotyping Master Mix is also compatible with KASP™ and Amplifluor® marker assays.

REQUIRED COMPONENTS

• qPCR machine or Thermocycler + Fluorescent plate reader
• PCR plate or equivalent and appropriate optically clear seal
• Template DNA
• PCR-grade water
• Genotyping assays

qPCR machine or Thermocycler + Fluorescent plate reader

PCR plate or equivalent and appropriate optically clear seal

Template DNA

PCR-grade water

Genotyping assays

K-INTDF

SKU: 700004305

100 assays per kit

Content:	100 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Dietary Fiber
Assay Format:	Enzymatic
Detection Method:	Gravimetric/HPLC
Signal Response:	Increase
Limit of Detection:	0.5 g/100 g
Total Assay Time:	~ 3 h work (over 2 days)
Application examples:	Food ingredients, food products and other materials.
Method recognition:	AACC Method 32-45.01, AACC Method 32-50.01, AOAC Method 2009.01 and AOAC Method 2011.25

The Integrated Total Dietary Fiber test kit is suitable for the measurement and analysis of Integrated Total Dietary Fiber.

Updated to include resistant, branched maltodextrins as a component in dietary fiber.

K-INTDF – An Integrated Procedure (AOAC Method 2009.01 & 2011.25) for the measurement of Total Dietary Fiber (including resistant starch and non-digestible oligosaccharides). This method combines the key attributes of AOAC Official Methods of Analysis 2002.02, 985.29, 991.43, 2001.03.

Specific dietary fiber fractions are measured as follows:

1. Insoluble dietary fiber (IDF), Higher Molecular Weight Soluble Dietary Fiber (SDFP) and Lower Molecular Weight Soluble Dietary Fiber (SDFS) determination (AOAC Method 2011.25)

2. Total High Molecular Weight Dietary Fiber (HMWDF) and SDFS determination (AOAC Method 2009.01)

The enzymes used in these methods are of very high purity; they are effectively devoid of contaminating enzymes active on β-glucan, pectin and arabinoxylan. 

Data calculators are located in the Documents tab.

See our full range of dietary fiber and starch products.

Validation of Methods

Advantages

• The only method that is consistent with the CODEX Alimentarius definition of dietary fiber
• High purity / standardised enzymes employed 
• All reagents stable for > 2 years 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
• Very competitive price (cost per test)

The Integrated Total Dietary Fiber test kit is suitable for the measurement and analysis of Integrated Total Dietary Fiber.

K-MANOL

SKU: 700004317

60 assays (manual) / 600 assays (microplate) / 580 assays (auto-analyser)

Content:	60 assays (manual) / 600 assays (microplate) / 580 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	D-Mannitol
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	5 to 75 μg of D-mannitol per assay
Limit of Detection:	0.50 mg/L
Reaction Time (min):	~ 4 min
Application examples:	Wine, chewing gum, dietetic foods, candies, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Novel method

The D-Mannitol test kit is suitable for the rapid and specific measurement and analysis of D-mannitol in food, beverages and other materials.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

View our complete list of alcohol assay kits.

Advantages

• Novel product (only enzymatic kit available) 
• Very cost effective 
• All reagents stable for > 2 years after preparation 
• Simple format 
• Rapid reaction 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included 
• Extended cofactors stability 
• Suitable for manual, microplate and auto-analyser formats

The D-Mannitol test kit is suitable for the rapid and specific measurement and analysis of D-mannitol in food, beverages and other materials.