
Bis-PEG23-endo-BCN is a click chemistry reagent, The BCN group enable copper free click chemitry with azide-tagged molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Bis-PEG23-endo-BCN is a click chemistry reagent, The BCN group enable copper free click chemitry with azide-tagged molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-PEG23-endo-BCN is a click chemistry reagent, The BCN group enable copper free click chemitry with azide-tagged molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Campylobacter jejuni is a curved, rod-shaped and microaerophillic gram negative bacterium. It is one of the most common causal agents of gastroenteritis with diarrhea as the main symptom. While infection of C. jejuni is seldom life-threatening, it is considered one of the most common food-borne bacteria with over 2 million people infected per year in US alone. Infection of C. jejuni usually results from consumption of poorly prepared food including undercooked meat (particularly poultry), untreated water or raw unpasteurized milk. Traditional identification of C. jejuni involves culturing, however the microaerophilic characteristic of this bacterium make the enrichment process laborious and costly.
Campylobacter jejuni TaqMan PCR Kit, 100 reactions
Campylobacter jejuni TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. TM36150 (100 preps) | Cat. TM36110 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
Campylobacter jejuni Primer & Probe Mix | 280 μL | 280 μL |
Campylobacter jejuni Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |
Tri(propargyl-NHCO-ethyloxyethyl)amine is a crosslinker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Tri(propargyl-NHCO-ethyloxyethyl)amine is a crosslinker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.
Specifications
Features | Specifications |
Main Functions | Isolation total RNA from FFPE tissue and section samples (with DNase) |
Applications | RT-PCR, quantitative RT-PCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation |
Purification method | Mini spin column |
Purification technology | Silica technology, DNase |
Process method | Manual (centrifugation or vacuum) |
Sample type | FFPE tissue sample |
Sample amount | 6mg |
Yield | 20μg |
Elution volume | ≥10μl |
Time per run | ≤60 minutes |
Liquid carrying volume per column | 800µl |
Binding yield of column | 100µg |
This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis with proteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-salt buffer.
Advantages
Kit Contents
Contents | R414402 | D414403 |
Purification Times | 50 Preps | 250 Preps |
HiPure RNA Micro Columns | 50 | 250 |
2ml Collection Tubes | 50 | 250 |
Buffer DPS | 60 ml | 250 ml |
Buffer FRL | 15 ml | 60 ml |
Buffer RLC | 15 ml | 60 ml |
Buffer RWC* | 10 ml | 50 ml |
Buffer RW2* | 20 ml | 2 x 50 ml |
DNase I | 600 µl | 5 x 600 µl |
DNase Booster Buffer | 1.5 ml | 6 ml |
Protease Dissolve Buffer | 1.8 ml | 10 ml |
Proteinase K | 24 mg | 120 mg |
RNase Free Water | 10 ml | 20 ml |
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Experiment Data
HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.
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