Bis-propargyl-PEG4 enables Click Chemistry reactions with azide compounds via copper catalyzed azide-alkyne Click Chemistry. The PEG units enhance the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-propargyl-PEG4 enables Click Chemistry reactions with azide compounds via copper catalyzed azide-alkyne Click Chemistry. The PEG units enhance the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The Opentrons Flat Bottom Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold flat bottom plates and other flat labware at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
The Opentrons Flat Bottom Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold flat bottom plates and other flat labware at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
Name of Product
Fasciola hepatica – IgG ELISA
Catalog Number
AF 9650
Short Info
Fasciolosis is one of the most frequently encountered autochthonous helminthic infections in Central Europe. The immunodiagnosis of Fasciola hepatica infections is challenged by high serological cross-reacitivity with other (hepatic) parasitological infections encountered in Central Europe, such as alveolar echinococcosis, toxocarosis and ascariosis, but also other parasitic infections acquired during overseas travel. The SAP-2 recombinant antigen shows a high specificity, especially with patients with other parasitic infections. It is a suitable serological test for routine diagnosis of human fasciolosis, particularly if the results are supported by clinical history.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
77% sensitivity, 98% specificity
Test Principle
The kit provides all the material needed to perform 96 enzyme-linked immunosorbant assays (ELISA) on breakable microtitration wells sensitized with Fasciola hepatica recombinant antigen. Specific antibodies in the sample will bind to the antigen and washing will remove unspecific antibodies. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate. A second washing step will remove unbound conjugate. Revealing bound antibodies is made by the addition of pNPP substrate which turns yellow in the presence of alkaline phosphatase. Color intensity is proportional to the amount of Fasciola hepatica specific antibodies in the sample. Potassium phosphate is added to stop the reaction. Absorbance at 405 nm is read using an ELISA microplate reader.The test can be performed with automatic systems, but this must be validated by the user.
Brief Instructions
Storage
2-8° C
Components
ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, stopping solution
12 x 8 strips (96 tests)
Fasciolosis is one of the most frequently encountered autochthonous helminthic infections in Central Europe. The immunodiagnosis of Fasciola hepatica infections is challenged by high serological cross-reacitivity with other (hepatic) parasitological infections encountered in Central Europe, such as alveolar echinococcosis, toxocarosis and ascariosis, but also other parasitic infections acquired during overseas travel. The SAP-2 recombinant antigen shows a high specificity, especially with patients with other parasitic infections. It is a suitable serological test for routine diagnosis of human fasciolosis, particularly if the results are supported by clinical history.
Permagen’s most universal ring magnet plate. From Low elution PCR applications up to 2 mL deep well, the X96 has you covered. No need to purchase two separate plates anymore. Smaller inner ring magnet allows for volumes as low as 5 µL, larger outer magnet handles up to 2 mL deep well
ANSI/ SBS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot on bottom, SBS/ SLAS fit on top to accept any microplate
Integrated Cushion base for maximum recovery. Helps aid in set-up, robot positioning inconsistencies, and labware consumable differences
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
A011623
A011623KT (Kit for 0.2 ml tubes)
Maximum – 2 mL (Deep Well)
Minimum – 5 µL (PCR Plate)
Permagen’s most universal ring magnet plate. From Low elution PCR applications up to 2 mL deep well, the X96 has you covered. No need to purchase two separate plates anymore. Smaller inner ring magnet allows for volumes as low as 5 µL, larger outer magnet handles up to 2 mL deep well
