Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings 150 reactions

Introduction

This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples such as blood, serum, plasma, swab soaking solution, fluid accumulation and homogenate solution. This kit is designed to remove host cells background nucleic acid and enrich pathogen nucleic acid (including viral/bacterial/fungal DNA/RNA) from the sample. Purified DNA/RNA is ready for downstream applications such as PCR, virus detection, tNGS and other related experiments.

Details

Specifications

Features	Specifications
Main Functions	Extract Pathogen RNA/DNA from 0.5-1.5ml whole blood, plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution for tNGS application, remove host background nucleic acid.
Applications	Real Time PCR, biochip analysis, NGS
Products	Pathogen DNA / RNA
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	whole blood, plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution
Sample amount	0.5 – 1.5 ml
Adaptive instrument	Nucleic acid extractor, pipetting workstation

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.

Kit Contents

Contents	R667200C	R667202C
Purification Times	24 Preps	96 Preps
2ml Bead Tube (0.4g)	24	96
DNase I (Powder)	10 mg	15 mg
DNase Buffer	5 ml	20 ml
Protease Dissolve Buffer	3 ml	8 ml
Lysis Buffer LBX1	40 ml	180 ml
Buffer TL	5 ml	20 ml
Proteinase K	24 mg	120 mg
MagBind Particles N9	1.2 ml	5 ml
Buffer MLB	30 ml	120 ml
Buffer MW1*	13 ml	110 ml
Buffer MW2*	10 ml	50 ml
Buffer AVE	10 ml	20 ml

Storage and Stability

Proteinase K, DNase I powder and MagPure Particles N9 should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples such as blood, serum, plasma, swab soaking solution, fluid accumulation and homogenate solution. This kit is designed to remove host cells background nucleic acid and enrich pathogen nucleic acid (including viral/bacterial/fungal DNA/RNA) from the sample. Purified DNA/RNA is ready for downstream applications such as PCR, virus detection, tNGS and other related experiments.

Overview

• RNA/microRNA/DNA/Proteins are preserved for more than 2 years at room temperature in Norgen’s Urine Preservative
• Compatible with most DNA, Total RNA, microRNA and protein isolation methods
• Preservative is available in a single dose liquid format (ampule)
• Preservative is also available in a dried format in tubes – Urine Collection and Preservation Tubes
• Urine Collection and Preservation Devices are available in 4 convenient sizes: 5 cc tubes, 15 cc tubes, 50 cc tubes, and 120 cc cups

Norgen’s Urine Preservative is designed for the rapid preservation of DNA, RNA, microRNA and proteins from fresh urine samples. In addition, the Urine Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins.

Norgen’s Urine Preservative is available in 2 convenient formats:

1.  Urine Preservative Single Dose Ampules
With this product Norgen’s Urine Preservative is supplied in a liquid format in single dose ampules. The user simply collects 5 – 50 mL of urine into a urine collection container and adds the contents of the Urine Preservative Single Dose (Cat# 18126). The urine and preservative are then mixed, and the urine nucleic acids and proteins are preserved at room temperature.

2.  Urine Collection and Preservation Tubes
Norgen’s Urine Preservative is also available in a dried format in Norgen’s Urine Collection and Preservation Tubes. The user simply collects urine into the tubes and mixes gently until the orange preservative pellet in the tube has dissolved. Norgen’s Urine Collection and Preservation Tubes are available in 3 convenient sizes:

• 5 cc tubes, 1 – 5 mL of urine
• 15 cc tubes, 5 – 15 mL of urine
• 50 cc tubes, 5 – 45 mL of urine
• 120 cc cup, 100 – 120 mL of urine

Details

Supporting Data

Figure 1 / 2

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Select Urine Tested with the Norgen Urine Collection and Preservation Tubes

Human
Mouse
Lynx
Wolf
Urine collected from snow

Available Sizes and Formats 

1. Urine Collection and Preservation Cups (120 cc) –  Urine inputs from 100 – 120 mL

Storage Conditions and Product Stability
All tubes/cups should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.

Kit Components	Cat. 18118 (50 tubes)	Cat. 18122 (50 tubes)	Cat. 18113 (50 tubes)	Cat. 18126 (50 tubes)	Cat. 18129 (1 cup)
Urine Collection and Preservation Tubes (5 cc)	50	–	–	–	–
Urine Collection and Preservation Tubes (15 cc)	–	50	–	–	–
Urine Collection and Preservation Tubes (50 cc)	–	–	50	–	–
Urine Preservative Single Dose	–	–	–	50	–
Urine Collection and Preservation Cup (120 cc)	–	–	–	–	1
ID Labels	50	–	–	50	–
Product Insert	1	1	1	1	1