Bis-propargyl-PEG4 enables Click Chemistry reactions with azide compounds via copper catalyzed azide-alkyne Click Chemistry. The PEG units enhance the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Bis-propargyl-PEG4 enables Click Chemistry reactions with azide compounds via copper catalyzed azide-alkyne Click Chemistry. The PEG units enhance the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
HCM066 PALCAM Agar Base
Product Info
Document
Product Info
Introduction
Intended Use
For the selective isolation and culture of Listeria monocytogenes.
Principle and Interpretation
Peptone provides the carbon and nitrogen sources necessary for growth; yeast extract powder and starch provide carbon and nitrogen sources, vitamins and growth factors; sodium chloride can maintain a balanced osmotic pressure; glucose provides a carbon source; Listeria hydrolyzes esculin and reacts with iron ions to form black 6,7-dihydroxycoumarin; mannitol is a fermentable sugar, and phenol red is a pH indicator; lithium chloride and other antibiotics can inhibit Gram-negative bacteria and most Gram-positive bacteria; agar is the coagulant of the culture medium.
Formulation
Ingredients
/liter
Peptone
23 g
Starch
1 g
NaCl
5 g
Columbia agar
13 g
Mannitol
10 g
Ferric ammonium citrate
0.5 g
Esculin (aesculin)
0.8 g
Dextrose (glucose)
0.5 g
Lithium chloride
15.0 g
Phenol red
0.08 g
pH7.2±0.2 at 25°C
Preparation
Weigh 68.9g of dry powder of this product, add 1L of distilled water or deionized water, stir, heat and boil until completely dissolved, divide into Erlenmeyer bottles, sterilize at 121℃ for 15min, cool to room temperature and set aside. Heat to dissolve agar before use, cool to 50℃, add 1 tube of supporting reagent (SR0140) per 100mL of basal culture medium, shake well and pour into sterilized culture dish.
Quality Control
The following quality control strains were inoculated and cultured at 35-37℃ for 24h. The results are as follows:
Quality control strains
Growth
Listeria monocytogenes ATCC19115
Gray-green colonies with a black depression in the center and black surrounding
Enterococcus faecalis ATCC29212
–
Escherichia coli ATCC25922
–
Storage and Shelf Life
2-30℃,Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Document
Intended Use For the selective isolation and culture of Listeria monocytogenes. Principle and Interpretation Peptone provides the carbon and nitrogen sources necessary for growth; yeast ex……
This kit can be used in quantitative and qualitative analysis of Pentachlorophenol residue in honey, milk, juice, and rice.
Document
This kit is based on ELISA technology, which is fast, easy, accurate and sensitive compared with common instrumental analysis and only needs 1.5 hours in one operation, it can considerably minimize operation error and work intensity.
EXTRAClean RNA Clean-Up and Concentration Micro-Elute Kit
Product Info
Document
Product Info
Overview
Ensure optimal results for sensitive applications like NGS
Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
Concentration of small amounts of RNA into 8 μL
Ideal for concentrating RNA purified from exosomes, plasma, serum, urine, and other bodily fluids and any RNA samples initially purified in large volumes
Efficient RNA cleanup from enzymatic reactions – labeling, DNase treatment and in vitro transcription
Cleanup of RNA isolated using different methods, including phenol/chloroform extractions
Fast and easy processing using rapid spin-column format
Suitable for all sizes of RNA, from large rRNA down to microRNA (miRNA)
No phenol or chloroform extractions
Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary separation matrix.
Norgen’s EXTRAClean RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration of up to 10 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. The minimum recommended elution volume is 8 μL, which enables the concentration of small amounts of all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other reaction components such as proteins, RNases and nucleotides, without the use of phenol or chloroform. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays and next generation sequencing.
Details
Kit Specifications (Spin Column)
Maximum Column Binding Capacity
10 μg
Size of RNA Purified
All sizes, including miRNA and small RNA (< 200 nt)