Bis-propargyl-PEG8 consists of two propargyl groups which can react with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry to form stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Bis-propargyl-PEG8 consists of two propargyl groups which can react with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry to form stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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P1154 HiPure Plasmid EF Mini Kit
Product Info
Document
Product Info
Introduction
The HiPure Plasmid EF Mini Kit combine the power of HiPure technology with Magen’s innovative endotoxin removal technology to deliver high-quality plasmid DNA with low endotoxin levels foruse in eukaryotic transfection, and in vitro experiments. The HiPure plasmid endo-free system uses a specially formulated buffer that prevents endotoxin molecules from binding to the surface of the HiPure matrix. Endotoxin contamination lowers transfection efficiencies for endotoxin sensitive celllines. For gene therapy, endotoxin contamination should be of major concernsince endotoxins have the potential to cause fever, endotoxin shock syndrome, and interfere with in vitro transfection into immune cells.
Details
Specifications
Features
Specifications
Main Functions
Isolation up to 80µg endotoxin-free plasmid DNA from 5-15ml bacterial culture. Recommend for low copy vector, Thoroughly remove RNA
Applications
Cell transfection, animal injection, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Low copy plasmid vector
Sample amount
5-15ml LB
Yield
10-70μg
Elution volume
≥75μl
Time per run
≤40 minutes
Liquid carrying volume per column
800μl
Binding yield of column
70μg
Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast – silica gel column purification is much faster than ion exchange
High yield – up to 70μg plasmid can be binded in one column
Low endotoxin content – the obtained plasmid can be directly used in cell transfection and animal injection
Kit Contents
Contents
P115402
P115403
Purification Times
50 Preps
250 Preps
RNase A
5 mg
20 mg
Buffer P1
30 ml
140 ml
Buffer P2
30 ml
140 ml
Buffer LEN3
15 ml
70 ml
Buffer LN4
50 ml
250 ml
Buffer LN5
30 ml
140 ml
Buffer PW1
30 ml
140 ml
Buffer PW2
12 ml
50 ml
Elution Buffer
15 ml
30 ml
HiPure DNA Mini Columns III
50
250
2 ml Collection Tubes
50
250
Storage and Stability
The kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2–8°C.
The HiPure Plasmid EF Mini Kit combine the power of HiPure technology with Magen’s innovative endotoxin removal technology to deliver high-quality plasmid DNA with low endotoxin levels foruse in eukaryotic transfection, and in vitro experiments. The HiPure plasmid endo-free system uses a specially formulated buffer that prevents endotoxin molecules from binding to the surface of the HiPure matrix. Endotoxin contamination lowers transfection efficiencies for endotoxin sensitive celllines. For gene therapy, endotoxin contamination should be of major concernsince endotoxins have the potential to cause fever, endotoxin shock syndrome, and interfere with in vitro transfection into immune cells.
genesig® kits are sold for research use only and are not licensed for diagnostic procedures.
Document
Exceptional value for money
Rapid detection of 2019-nCoV
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
The ITS2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the fungal target ITS2 libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XPMagneticBeads(supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The ITS2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the ITS2 region of the fungal DNA. The post-PCR reaction is then cleaned up using AMPure XPbeads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XPbeads. The libraries are then ready for quantification, pooling and sequencing.
Storage Conditions and Product Stability Norgen’s ITS2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components will remain stable for at least 1 year when stored at the specified storage conditions.